RNA interference-mediated silencing of VEGF and bFGF suppresses endostatin secretion in pancreatic carcinoma cells

Yan,Changqing; Wang,Chuan; Dong,Mei; Liu,Sanguang; Qi,Cheng; Zhao,Yupei

doi:10.3892/ol.2013.1102

March 2013 Volume 5 Issue 3

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March 2013 Volume 5 Issue 3

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Article

RNA interference-mediated silencing of VEGF and bFGF suppresses endostatin secretion in pancreatic carcinoma cells

Authors:
- Changqing Yan
- Chuan Wang
- Mei Dong
- Sanguang Liu
- Cheng Qi
- Yupei Zhao
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Affiliations: Department of Heptobiliary Surgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, P.R. China, Department of Pharmacology, Hebei Medical University, Shijiazhuang 050017, P.R. China, Department of Surgery, The Affiliated Hospital of Hebei University of Science and Technology, Shijiazhuang 050018, P.R. China, Department of General Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Science, Beijing 100730, P.R. China
Pages: 1031-1035
|
Published online on: January 2, 2013

https://doi.org/10.3892/ol.2013.1102
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Abstract

Pro-angiogenic factors [vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF)] and anti-angiogenic factors (endostatin) play important roles in the progression of pancreatic cancer. The purpose of the present study was to investigate the knockdown effect by either VEGF or bFGF siRNA on the expression and secretion of endostatin in pancreatic carcinoma cells. Pancreatic carcinoma cell lines (sw1990, Panc-1 and PCT-3) were treated with VEGF and bFGF siRNA. The expression of VEGF, bFGF and endostatin in pancreatic carcinoma cell lines was determined by reverse transcription-polymerase chain reaction (RT-PCR) and western blot analysis. Secretion of endostatin was measured by enzyme-linked immunosorbent assay (ELISA). bFGF and VEGF siRNA significantly reduced the expression of bFGF and VEGF mRNA, respectively, but did not affect mRNA and protein expression of endostatin in pancreatic carcinoma cell lines. However, secretion of endostatin in PCT-3, Panc-1 and sw1990 cells was significantly inhibited by bFGF and VEGF siRNA. This study demonstrated that pro-angiogenic factors (VEGF and bFGF) differentially modulate expression and secretion of anti-angiogenic factors (endostatin). This result may have important implications in the anti-angiogenesis therapy in pancreatic cancer.

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1	Yan CQ, Zhao YP, Dai MH, et al: The relationship between the changes of proangiogenic factors serum concentrations and progression of pancreatic carcinoma patients. Zhonghua Wai Ke Za Zhi. 45:496–498. 2007.(In Chinese).
2	Dai MH, Yan CQ, Zhao YP and Song YM: Relationship between changes of serum concentrations of antiangiogenic factors and disease progression in patients of pancreatic carcinoma. Zhonghua Wai Ke Za Zhi. 45:1199–1201. 2007.(In Chinese).
3	Yan CQ and Zhao YP: Expression of angiogenic factors in pancreatic carcinoma cell and their significance. Zhonghua Wai Ke Za Zhi. 47:787–790. 2009.(In Chinese).
4	Yan CQ and Zhao YP: The significance of VEGF siRNA and bFGF siRNA in invasion and proliferation of pancreatic carcinoma cell. Zhonghua Wai Ke Za Zhi. 48:610–614. 2010.(In Chinese).
5	Sakurai T and Kudo M: Signaling pathways governing tumor angiogenesis. Oncology. 81(Suppl 1): 24–29. 2011. View Article : Google Scholar
6	Ohlund D, Ardnor B, Oman M, et al: Expression pattern and circulating levels of endostatin in patients with pancreas cancer. Int J Cancer. 122:2805–2810. 2008. View Article : Google Scholar : PubMed/NCBI
7	Wigmore SJ: Endostatin in the pancreas. Br J Cancer. 92:5–6. 2005. View Article : Google Scholar
8	Rahbari NN, Schmidt T, Falk CS, et al: Expression and prognostic value of circulating angiogenic cytokines in pancreatic cancer. BMC Cancer. 11:2862011. View Article : Google Scholar : PubMed/NCBI
9	Jain RK: Normalization of tumor vasculature: an emerging concept in antiangiogenic therapy. Science. 307:58–62. 2005. View Article : Google Scholar : PubMed/NCBI
10	Eichholz A, Merchant S and Gaya AM: Anti-angiogenesis therapies: their potential in cancer management. Onco Targets Ther. 3:69–82. 2010.PubMed/NCBI
11	Korc M: Pathways for aberrant angiogenesis in pancreatic cancer. Mol Cancer. 2:82003. View Article : Google Scholar
12	Benckert C, Thelen A, Cramer T, et al: Impact of microvessel density on lymph node metastasis and survival after curative resection of pancreatic cancer. Surg Today. 42:169–176. 2012. View Article : Google Scholar : PubMed/NCBI
13	Brammer RD, Bramhall SR and Eggo MC: Endostatin expression in a pancreatic cell line is modulated by a TNFalpha-dependent elastase. Br J Cancer. 93:1024–1028. 2005. View Article : Google Scholar : PubMed/NCBI
14	Heljasvaara R, Nyberg P, Luostarinen J, et al: Generation of biologically active endostatin fragments from human collagen XVIII by distinct matrix metalloproteases. Exp Cell Res. 307:292–304. 2005. View Article : Google Scholar : PubMed/NCBI
15	Nilsson UW, Garvin S and Dabrosin C: MMP-2 and MMP-9 activity is regulated by estradiol and tamoxifen in cultured human breast cancer cells. Breast Cancer Res Treat. 102:253–261. 2007. View Article : Google Scholar : PubMed/NCBI
16	Zhong J, Gencay MM, Bubendorf L, et al: ERK1/2 and p38 MAP kinase control MMP-2, MT1-MMP, and TIMP action and affect cell migration: a comparison between mesothelioma and mesothelial cells. J Cell Physiol. 207:540–552. 2006. View Article : Google Scholar : PubMed/NCBI
17	Xie L, Duncan MB, Pahler J, et al: Counterbalancing angiogenic regulatory factors control the rate of cancer progression and survival in a stage-specific manner. Proc Natl Acad Sci USA. 108:9939–9944. 2011. View Article : Google Scholar
18	Albrecht I, Kopfstein L, Strittmatter K, et al: Suppressive effects of vascular endothelial growth factor-B on tumor growth in a mouse model of pancreatic neuroendocrine tumorigenesis. PLoS One. 5:e141092010. View Article : Google Scholar
19	Pereira ER, Liao N, Neale GA and Hendershot LM: Transcriptional and post-transcriptional regulation of proangiogenic factors by the unfolded protein response. PLoS One. 5:e125212010. View Article : Google Scholar : PubMed/NCBI
20	Schuch G, Kisker O, Atala A and Soker S: Pancreatic tumor growth is regulated by the balance between positive and negative modulators of angiogenesis. Angiogenesis. 5:181–190. 2002. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

RNA interference-mediated silencing of VEGF and bFGF suppresses endostatin secretion in pancreatic carcinoma cells

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