Migration of gastric cancer cells in response to lysophosphatidic acid is mediated by LPA receptor 2

  • Authors:
    • Dezhi Yang
    • Wenhua Yang
    • Qian Zhang
    • Yan Hu
    • Liang Bao
    • Alatangaole Damirin
  • View Affiliations

  • Published online on: January 7, 2013     https://doi.org/10.3892/ol.2013.1107
  • Pages: 1048-1052
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Abstract

Lysophosphatidic acid (LPA), a natural phospholipid, is able to modulate diverse cellular responses through LPA receptors (LPARs). Several studies have reported that LPAR2 gene expression is increased in a variety of cancer cells, suggesting that LPAR2 is involved in gastric cancer. The present study investigated the expression profiles of the LPAR and involvement of the receptor subtypes in the LPA‑induced migration of gastric cancer cells using cell migration assays, RNA interference, quantitative real‑time PCR and western blotting. LPAR2 was observed to be highly expressed in SGC‑7901 cells, a human gastric cancer cell line, while LPAR1 and LPAR3 were not. Transient transfection with LPAR2 siRNA was observed to reduce LPAR2 mRNA in SGC‑7901 cells and eliminate the LPA‑induced cell migration. It was also observed that LPA‑induced SGC‑7901 cell migration was inhibited by the inhibitor for Gq/11 protein and p38. The results suggest that the LPAR2/Gq/11/p38 pathway regulates LPA‑induced SGC‑7901 cell migration. The present findings suggest that LPAR2 may be a potential target for the clinical treatment of gastric cancer.
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March 2013
Volume 5 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Yang D, Yang W, Zhang Q, Hu Y, Bao L and Damirin A: Migration of gastric cancer cells in response to lysophosphatidic acid is mediated by LPA receptor 2. Oncol Lett 5: 1048-1052, 2013
APA
Yang, D., Yang, W., Zhang, Q., Hu, Y., Bao, L., & Damirin, A. (2013). Migration of gastric cancer cells in response to lysophosphatidic acid is mediated by LPA receptor 2. Oncology Letters, 5, 1048-1052. https://doi.org/10.3892/ol.2013.1107
MLA
Yang, D., Yang, W., Zhang, Q., Hu, Y., Bao, L., Damirin, A."Migration of gastric cancer cells in response to lysophosphatidic acid is mediated by LPA receptor 2". Oncology Letters 5.3 (2013): 1048-1052.
Chicago
Yang, D., Yang, W., Zhang, Q., Hu, Y., Bao, L., Damirin, A."Migration of gastric cancer cells in response to lysophosphatidic acid is mediated by LPA receptor 2". Oncology Letters 5, no. 3 (2013): 1048-1052. https://doi.org/10.3892/ol.2013.1107