Epithelial‑mesenchymal transition mediates anoikis resistance and enhances invasion in pleural effusion‑derived human lung cancer cells

Chunhacha,Preedakorn; Sriuranpong,Virote; Chanvorachote,Pithi

doi:10.3892/ol.2013.1108

March 2013 Volume 5 Issue 3

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

March 2013 Volume 5 Issue 3

Full Size Image

Article

Epithelial‑mesenchymal transition mediates anoikis resistance and enhances invasion in pleural effusion‑derived human lung cancer cells

Authors:
- Preedakorn Chunhacha
- Virote Sriuranpong
- Pithi Chanvorachote
View Affiliations / Copyright

Affiliations: Department of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand, Department of Medicine, Division of Medical Oncology, Faculty of Medicine, Chulalongkorn University and The King Chulalongkorn Memorial Hospital, Bangkok 10330, Thailand
Pages: 1043-1047
|
Published online on: January 7, 2013

https://doi.org/10.3892/ol.2013.1108
Expand metrics +

Abstract

Epithelial‑mesenchymal transition (EMT) is implicated in cancer pathological processes, particularly cancer invasion and metastasis. The present study demonstrated that EMT was critical for the metastasic potential of lung cancer cells isolated from a patient. P1 primary lung cancer cells were found to exhibit increased anoikis resistance compared with established A549, H23 and H460 lung cancer cells. Results of migration and invasion assays revealed that the invasion capability of P1 and A549 cells was higher than that of H23 and H460 cells. However, the migration of P1 cells was similar to that of H23 and H460 cells while A549 demonstrated a superior migrating ability. Western blot analysis indicated that while E‑cadherin levels in all lung cancer cells were identified as comparable, P1 cells expressed the highest levels of N‑cadherin. In the present study, detachment of cells was demonstrated for the first time to stimulate further transition of E‑cadherin to N‑cadherin. In addition, this obervation was more pronounced in P1 cells. These observations highlight the importance of EMT in cancer metastasis. In order to study the effect of ethnicity on cancer cell behavior, in the future a large number of Thai patient-derived cell lines must be analyzed.

View Figures

View References

1	Kalluri R and Weinberg RA: The basics of epithelial-mesenchymal transition. J Clin Invest. 119:1420–1428. 2009. View Article : Google Scholar : PubMed/NCBI
2	Yilmaz M and Christofori G: EMT, the cytoskeleton and cancer cell invasion. Cancer Metastasis Rev. 28:15–33. 2009. View Article : Google Scholar : PubMed/NCBI
3	Kalluri R and Neilson EG: Epithelial mesenchymal transition and its implications for fibrosis. J Clin Invest. 112:1776–1784. 2003. View Article : Google Scholar : PubMed/NCBI
4	Guadamillas MC, Cerezo A and Del Pozo MA: Overcoming anoikis - pathways to anchorage-independent growth in cancer. J Cell Sci. 124:3189–3197. 2011. View Article : Google Scholar : PubMed/NCBI
5	Boisvert AK, Longmate W, Abel EV and Aplin A: Mcl-1 is required for melanoma cell resistance to anoikis. Mol Cancer Res. 7:549–556. 2009. View Article : Google Scholar : PubMed/NCBI
6	Chiarugi P and Giannoni E: Anoikis: a necessary death program for anchorage-dependent cells. Biochem Pharmacol. 76:1352–1364. 2008. View Article : Google Scholar : PubMed/NCBI
7	Li G, Satyamoorthy K and Herlyn M: N-cadherin-mediated intercellular interactions promote survival and migration of melanoma cells. Cancer Res. 61:3819–3825. 2001.PubMed/NCBI
8	Minard ME, Ellis LM and Gallick GE: Tiam1 regulates cell adhesion, migration and apoptosis in colon tumor cells. Clin Exp Metastasis. 23:301–313. 2006. View Article : Google Scholar : PubMed/NCBI
9	Hazan RB, Qiao R, Keren R, Badano I and Suyama K: Cadherin switch in tumor progression. Ann NY Acad Sci. 1014:155–163. 2004. View Article : Google Scholar : PubMed/NCBI
10	Ko H, Kim S, Jin CH, Lee E, Ham S, Yook JI and Kim K: Protein kinase casein kinase 2-mediated upregulation of N-cadherin confers anoikis resistance on esophageal carcinoma cells. Mol Cancer Res. 10:1032–1038. 2012. View Article : Google Scholar : PubMed/NCBI
11	Vatanasapt V, Sriamporn S and Vatanasapt P: Cancer control in Thailand. Jpn J Clin Oncol. 32:S82–S91. 2002. View Article : Google Scholar
12	Thompson EW and Newgreen DF: Carcinoma invasion and metastasis: a role for epithelial-mesenchymal transition? Cancer Res. 65:5991–5995. 2005. View Article : Google Scholar : PubMed/NCBI
13	Luanpitpong S, Talbott SJ, Rojanasakul Y, Nimmannit U, Pongrakhananon V, Wang L and Chanvorachote P: Regulation of lung cancer cell migration and invasion by reactive oxygen species and caveolin-1. J Biol Chem. 285:38832–38840. 2010. View Article : Google Scholar : PubMed/NCBI
14	Christiansen JJ and Rajasekaran AK: Reassessing epithelial to mesenchymal transition as a prerequisite for carcinoma invasion and metastasis. Cancer Res. 66:8319–8326. 2006. View Article : Google Scholar : PubMed/NCBI
15	Taweevisit M, Chirakalwasan N, Pumsuk U, Keelawat S and Shuangshoti S: Metastatic adenocarcinoma to the cervical lymph node: a significant proportion of cholangiocarcinoma in Thai patients. Asian Pac J Cancer Prev. 9:39–41. 2008.PubMed/NCBI
16	Chanvorachote P, Luanpitpong S, Chunhacha P, Promden W and Sriuranpong V: Expression of CA125 and cisplatin susceptibility of pleural effusion-derived human lung cancer cells from a Thai patient. Oncol Lett. 4:252–256. 2012.PubMed/NCBI
17	Tsuji T, Ibaragi S and Hu GF: Epithelial-mesenchymal transition and cell cooperativity in metastasis. Cancer Res. 69:7135–7139. 2009. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Epithelial‑mesenchymal transition mediates anoikis resistance and enhances invasion in pleural effusion‑derived human lung cancer cells

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Related Articles