Suppression of NF-κB activity by mutant IκBα: A molecular target for radiosensitization of adenoid cystic carcinoma

  • Authors:
    • Zhe Liu
    • Shengyun Huang
    • Shizhou Zhang
    • Jiawen Si
    • Qiang Wang
    • Qiangxiu Wang
    • Wenli Mu
    • Junqing Han
    • Dongsheng Zhang
  • View Affiliations

  • Published online on: January 24, 2013     https://doi.org/10.3892/ol.2013.1153
  • Pages: 1375-1381
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The constitutive activation of the nuclear factor κB (NF-κB) signaling pathway is involved in oncogenesis, invasive growth, metastasis and induced resistance to radiation and chemotherapy. Selective inhibition of the NF-κB signaling pathway, either by a mutant inhibitor or pharmacological agents, improves the therapeutic efficiency of irradiation. In the present study, the changes in NF-κB expression and the rate of apoptosis were investigated following irradiation of cells of an adenoid cystic carcinoma cell line (ACC-M) in which NF-κB expression had been inhibited by transient transfection with a mutant IκBα plasmid. ACC-M cells were transiently transfected with the mutant IκBα plasmid using Lipofectamine and the expression of this mutant IκBα gene was verified. The presence of the mutant IκBα gene alone did not result in a reduction in cell proliferation. Furthermore, a significant inhibition of translocation and synthesis of NF-κB protein in the transfected cells was observed after irradiation. NF-κB protein was activated by different doses of irradiation in a dose- and time-dependent manner with concordant changes in the radiosensitivity of ACC-M cells. We conclude that the mutant IκBα gene selectively inhibited the NF-κB pathway, which may be a promising method to improve the radiosensitivity of adenoid cystic carcinomas.
View Figures
View References

Related Articles

Journal Cover

April 2013
Volume 5 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Liu Z, Huang S, Zhang S, Si J, Wang Q, Wang Q, Mu W, Han J and Zhang D: Suppression of NF-κB activity by mutant IκBα: A molecular target for radiosensitization of adenoid cystic carcinoma. Oncol Lett 5: 1375-1381, 2013
APA
Liu, Z., Huang, S., Zhang, S., Si, J., Wang, Q., Wang, Q. ... Zhang, D. (2013). Suppression of NF-κB activity by mutant IκBα: A molecular target for radiosensitization of adenoid cystic carcinoma. Oncology Letters, 5, 1375-1381. https://doi.org/10.3892/ol.2013.1153
MLA
Liu, Z., Huang, S., Zhang, S., Si, J., Wang, Q., Wang, Q., Mu, W., Han, J., Zhang, D."Suppression of NF-κB activity by mutant IκBα: A molecular target for radiosensitization of adenoid cystic carcinoma". Oncology Letters 5.4 (2013): 1375-1381.
Chicago
Liu, Z., Huang, S., Zhang, S., Si, J., Wang, Q., Wang, Q., Mu, W., Han, J., Zhang, D."Suppression of NF-κB activity by mutant IκBα: A molecular target for radiosensitization of adenoid cystic carcinoma". Oncology Letters 5, no. 4 (2013): 1375-1381. https://doi.org/10.3892/ol.2013.1153