Suppression of NF-κB activity by mutant IκBα: A molecular target for radiosensitization of adenoid cystic carcinoma

Liu,Zhe; Huang,Shengyun; Zhang,Shizhou; Si,Jiawen; Wang,Qiang; Wang,Qiangxiu; Mu,Wenli; Han,Junqing; Zhang,Dongsheng

doi:10.3892/ol.2013.1153

April 2013 Volume 5 Issue 4

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April 2013 Volume 5 Issue 4

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Article

Suppression of NF-κB activity by mutant IκBα: A molecular target for radiosensitization of adenoid cystic carcinoma

Authors:
- Zhe Liu
- Shengyun Huang
- Shizhou Zhang
- Jiawen Si
- Qiang Wang
- Qiangxiu Wang
- Wenli Mu
- Junqing Han
- Dongsheng Zhang
View Affiliations / Copyright

Affiliations: Department of Oral and Maxillofacial Surgery, Provincial Hospital Affiliated to Shandong University, Jinan 250021, P.R. China, Department of Pathology, Provincial Hospital Affiliated to Shandong University, Jinan 250021, P.R. China, Department of Medical Research Center, Provincial Hospital Affiliated to Shandong University, Jinan 250021, P.R. China, Department of Cancer Center, Provincial Hospital Affiliated to Shandong University, Jinan 250021, P.R. China
Pages: 1375-1381
|
Published online on: January 24, 2013

https://doi.org/10.3892/ol.2013.1153
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Abstract

The constitutive activation of the nuclear factor κB (NF-κB) signaling pathway is involved in oncogenesis, invasive growth, metastasis and induced resistance to radiation and chemotherapy. Selective inhibition of the NF-κB signaling pathway, either by a mutant inhibitor or pharmacological agents, improves the therapeutic efficiency of irradiation. In the present study, the changes in NF-κB expression and the rate of apoptosis were investigated following irradiation of cells of an adenoid cystic carcinoma cell line (ACC-M) in which NF-κB expression had been inhibited by transient transfection with a mutant IκBα plasmid. ACC-M cells were transiently transfected with the mutant IκBα plasmid using Lipofectamine and the expression of this mutant IκBα gene was verified. The presence of the mutant IκBα gene alone did not result in a reduction in cell proliferation. Furthermore, a significant inhibition of translocation and synthesis of NF-κB protein in the transfected cells was observed after irradiation. NF-κB protein was activated by different doses of irradiation in a dose- and time-dependent manner with concordant changes in the radiosensitivity of ACC-M cells. We conclude that the mutant IκBα gene selectively inhibited the NF-κB pathway, which may be a promising method to improve the radiosensitivity of adenoid cystic carcinomas.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Suppression of NF-κB activity by mutant IκBα: A molecular target for radiosensitization of adenoid cystic carcinoma

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