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Article

Synuclein‑γ suppression mediated by RNA interference inhibits the clonogenicity and invasiveness of MCF‑7 cells

  • Authors:
    • Bo Liang
    • Xin‑Jun Wang
    • Pei‑Hong Shen
    • Xue‑Yuan Li
    • Hong‑Wei Cheng
    • Qiao Shan
    • Kui‑Yuan Guo
    • Yu‑Wen Cao
    • Qing‑Xia Fan
    • Rui‑Feng Zheng
    • Bei Li
    • Wei Zhang
    • Yan‑Wei Li
    • Kai Yang
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, The Fifth Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, Department of Pathology, The Fifth Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, Department of Experimental Therapeutics, BC Cancer Agency Research Centre, Vancouver, British Columbia V5Z1L3, Canada, Department of General Surgery, The Fifth Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, Department of Oncology, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan 450051, P.R. China, Center of Cancer Therapy, Henan People's Armed Police Corps Hospital, Zhengzhou, Henan 450052, P.R. China
  • Pages: 1347-1352
    |
    Published online on: February 14, 2013
       https://doi.org/10.3892/ol.2013.1192
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Abstract

The aim of the present study was to investigate the effects of synuclein‑γ (SNCG) downregulation by RNA interference (RNAi) on the clonogenicity and invasiveness of MCF‑7 breast cancer cells. This study used four pairs of SNCG‑specific siRNAs which were designed and cloned into the pGPU6 plasmid for introduction into an MCF‑7 cell line. The SNCG knockdown efficacies of the four siRNAs were compared using the reverse transcription polymerase chain reaction (RT‑PCR) and immunocytochemistry. The cells' clonogenic and invasive phenotypes were examined with clonogenic and Boyden chamber assays. In comparison with the non‑specific siRNA and empty vector controls, all four SNCG siRNAs were observed to significantly inhibit SNCG expression at the mRNA and protein levels (F=481.06, P<0.001; F=147.42, P<0.0001). SNCG suppression mediated by RNAi successfully inhibited the clonogenicity (P=0.002) and invasiveness (P<0.001) of transfected MCF‑7 cells. According to the results of the present study, we concluded that SNCG suppression mediated by RNAi significantly suppressed SNCG expression at the mRNA and protein levels, suggesting that SNCG suppression mediated by an RNAi strategy may become a novel approach for treating advanced breast cancer.
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1 

Coughlin SS and Ekwueme DU: Breast cancer as a global health concern. Cancer Epidemiol. 33:315–318. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Garnock-Jones KP, Keating GM and Scott LJ: Trastuzumab: A review of its use as adjuvant treatment in human epidermal growth factor receptor 2 (HER2)-positive early breast cancer. Drugs. 70:215–239. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Lavedan C, Leroy E, Dehejia A, et al: Identification, localization and characterization of the human gamma-synuclein gene. Hum Genet. 103:106–112. 1998. View Article : Google Scholar : PubMed/NCBI

4 

Surgucheva I, Weisman AD, Goldberg JL, et al: γ-Synuclein as a marker of retinal ganglion cells. Mol Vis. 14:1540–1548. 2008.

5 

George JM: The synucleins. Genome Biol 3: REVIEWS3002. 2002

6 

Ahmad M, Attoub S, Singh MN, et al: Gamma-synuclein and the progression of cancer. FASEB. 21:3419–3430. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Shen PH, Fan QX, Li YW, et al: SNCG shRNA suppressed breast cancer cell xenograft formation and growth in nude mice. Chinese Med J (Engl). 124:1524–1528. 2011.PubMed/NCBI

8 

Lavedan C: The synuclein family. Genome Res. 8:871–880. 1998.

9 

Von Bohlen und Halbach O: Synucleins and their relationship to Parkinson’s disease. Cell Tissue Res. 318:163–174. 2004.

10 

Iwai A: Properties of NACP/alpha-synuclein and its role in Alzheimer’s disease. Biochim Biophys Acta. 1502:95–109. 2000.PubMed/NCBI

11 

Ji H, Liu Y, Jia T, et al: Identification of a breast cancer-specific gene, BCSG1, by direct differential cDNA sequencing. Cancer Res. 57:759–764. 1997.PubMed/NCBI

12 

Bruening W, Giasson BI, Klein-Szanto AJ, et al: Synucleins are expressed in the majority of breast and ovarian carcinomas and in preneoplastic lesions of the ovary. Cancer. 88:2154–2163. 2000. View Article : Google Scholar : PubMed/NCBI

13 

Zhao W, Liu H, Liu W, et al: Abnormal activation of the synuclein-gamma gene in hepatocellular carcinomas by epigenetic alteration. Int J Oncol. 28:1081–1088. 2006.PubMed/NCBI

14 

Zhou CQ, Liu S, Xue LY, et al: Down-regulation of gamma-synuclein in human esophageal squamous cell carcinoma. World J Gastroenterol. 9:1900–1903. 2003.PubMed/NCBI

15 

Liu H, Liu W, Wu Y, et al: Loss of epigenetic control of synuclein-gamma gene as a molecular indicator of metastasis in a wide range of human cancers. Cancer Res. 65:7635–7643. 2005.PubMed/NCBI

16 

Singh VK and Jia Z: Targeting synuclein-gamma to counteract drug resistance in cancer. Expert Opin Ther Targets. 12:59–68. 2008. View Article : Google Scholar : PubMed/NCBI

17 

Liu J, Spence MJ, Zhang LY, et al: Transcriptional suppression of synuclein gamma (SNCG) expression in human breast cancer cells by the growth inhibitory cytokine oncostatin M. Breast Cancer Res Treat. 62:99–107. 2002. View Article : Google Scholar : PubMed/NCBI

18 

Lu A, Zhang F, Gupta A and Liu L: Blockade of AP1 trans-activation abrogates the abnormal expression of breast cancer-specific gene 1 in breast cancer cells. J Biol Chem. 277:31364–31372. 2002. View Article : Google Scholar : PubMed/NCBI

19 

Jiang Y, Liu Y, Goldberg ID and Shi YE: Gamma synuclein, a novel heat-shock protein-associated chaperone, stimulates ligand-dependent estrogen receptor alpha signaling and mammary tumorigenesis. Cancer Res. 64:4539–4546. 2004. View Article : Google Scholar

20 

Jiang Y, Liu Y, Lu A, et al: Stimulation of estrogen receptor signaling by gamma synuclein. Cancer Res. 63:3899–3903. 2003.PubMed/NCBI

21 

Ashihara E, Kawata E and Maekawa T: Future prospect of RNA interference for cancer therapies. Curr Drug Targets. 11:345–360. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Krajewska M, Krajewski S, Epstein JI, et al: Immunohistochemical analysis of bcl-2, bax, bcl-X, and mcl-1 expression in prostate cancers. Am J Pathol. 148:1567–1576. 1996.PubMed/NCBI

23 

Guo J, Shou C, Meng L, et al: Neuronal protein synuclein gamma predicts poor clinical outcome in breast cancer. Int J Cancer. 121:1296–1305. 2007. View Article : Google Scholar : PubMed/NCBI

24 

Jia T, Liu YE, Liu J and Shi YE: Stimulation of breast cancer invasion and metastasis by synuclein gamma. Cancer Res. 59:742–747. 1999.PubMed/NCBI

25 

Gupta A, Inaba S, Wong OK, et al: Breast cancer-specific gene 1 interacts with the mitotic checkpoint kinase BubR1. Oncogene. 22:7593–7599. 2003. View Article : Google Scholar : PubMed/NCBI

26 

Inaba S, Li C, Shi YE, et al: Synuclein gamma inhibits the mitotic checkpoint function and promotes chromosomal instability of breast cancer cells. Breast Cancer Res Treat. 94:25–35. 2005. View Article : Google Scholar : PubMed/NCBI

27 

Singh VK, Zhou Y, Marsh JA, et al: Synuclein-gamma targeting peptide inhibitor that enhances sensitivity of breast cancer cells to antimicrotubule drugs. Cancer Res. 67:626–633. 2007. View Article : Google Scholar : PubMed/NCBI

28 

Zhou Y, Inaba S and Liu J: Inhibition of synuclein-γ expression increases the sensitivity of breast cancer cells to paclitaxel treatment. Int J Oncol. 29:289–295. 2006.

29 

Surgucheva IG, Sivak JM, Fini ME, et al: Effect of gamma-synuclein overexpression on matrix metalloproteinases in retinoblastoma Y79 cells. Arch Biochem Biophys. 410:167–176. 2003. View Article : Google Scholar : PubMed/NCBI

30 

Liu H, Liu W, Wu Y, et al: Loss of epigenetic control of synuclein-gamma gene as a molecular indicator of metastasis in a wide range of human cancers. Cancer Res. 65:7635–7643. 2005.PubMed/NCBI

31 

Martin TA, Gomez K, Watkins G, et al: Expression of breast cancer specific gene-1 (BCSG-1/γ-synuclein) is associated with tumor grade but not with clinical outcome of patients with breast cancer. Oncol Rep. 16:207–212. 2006.PubMed/NCBI

32 

Wu K, Weng Z, Tao Q, et al: Stage specific expression of breast cancer specific gene γ-synuclein. Cancer Epidemiol Biomark Prev. 12:920–925. 2003.

33 

Dang RL, Shen FH, Xia QA, et al: Expression of γ-synuclein protein in ovarian cancer. Mod Prev Med. 36:3551–3553. 2009.

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Copy and paste a formatted citation
Spandidos Publications style
Liang B, Wang XJ, Shen PH, Li XY, Cheng HW, Shan Q, Guo KY, Cao YW, Fan QX, Zheng RF, Zheng RF, et al: Synuclein‑γ suppression mediated by RNA interference inhibits the clonogenicity and invasiveness of MCF‑7 cells. Oncol Lett 5: 1347-1352, 2013.
APA
Liang, B., Wang, X., Shen, P., Li, X., Cheng, H., Shan, Q. ... Yang, K. (2013). Synuclein‑γ suppression mediated by RNA interference inhibits the clonogenicity and invasiveness of MCF‑7 cells. Oncology Letters, 5, 1347-1352. https://doi.org/10.3892/ol.2013.1192
MLA
Liang, B., Wang, X., Shen, P., Li, X., Cheng, H., Shan, Q., Guo, K., Cao, Y., Fan, Q., Zheng, R., Li, B., Zhang, W., Li, Y., Yang, K."Synuclein‑γ suppression mediated by RNA interference inhibits the clonogenicity and invasiveness of MCF‑7 cells". Oncology Letters 5.4 (2013): 1347-1352.
Chicago
Liang, B., Wang, X., Shen, P., Li, X., Cheng, H., Shan, Q., Guo, K., Cao, Y., Fan, Q., Zheng, R., Li, B., Zhang, W., Li, Y., Yang, K."Synuclein‑γ suppression mediated by RNA interference inhibits the clonogenicity and invasiveness of MCF‑7 cells". Oncology Letters 5, no. 4 (2013): 1347-1352. https://doi.org/10.3892/ol.2013.1192
Copy and paste a formatted citation
x
Spandidos Publications style
Liang B, Wang XJ, Shen PH, Li XY, Cheng HW, Shan Q, Guo KY, Cao YW, Fan QX, Zheng RF, Zheng RF, et al: Synuclein‑γ suppression mediated by RNA interference inhibits the clonogenicity and invasiveness of MCF‑7 cells. Oncol Lett 5: 1347-1352, 2013.
APA
Liang, B., Wang, X., Shen, P., Li, X., Cheng, H., Shan, Q. ... Yang, K. (2013). Synuclein‑γ suppression mediated by RNA interference inhibits the clonogenicity and invasiveness of MCF‑7 cells. Oncology Letters, 5, 1347-1352. https://doi.org/10.3892/ol.2013.1192
MLA
Liang, B., Wang, X., Shen, P., Li, X., Cheng, H., Shan, Q., Guo, K., Cao, Y., Fan, Q., Zheng, R., Li, B., Zhang, W., Li, Y., Yang, K."Synuclein‑γ suppression mediated by RNA interference inhibits the clonogenicity and invasiveness of MCF‑7 cells". Oncology Letters 5.4 (2013): 1347-1352.
Chicago
Liang, B., Wang, X., Shen, P., Li, X., Cheng, H., Shan, Q., Guo, K., Cao, Y., Fan, Q., Zheng, R., Li, B., Zhang, W., Li, Y., Yang, K."Synuclein‑γ suppression mediated by RNA interference inhibits the clonogenicity and invasiveness of MCF‑7 cells". Oncology Letters 5, no. 4 (2013): 1347-1352. https://doi.org/10.3892/ol.2013.1192
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