Quercetin liposomes protect against radiation‑induced pulmonary injury in a murine model

Liu,Hao; Xue,Jian‑Xing; Li,Xing; Ao,Rui; Lu,You

doi:10.3892/ol.2013.1365

Quercetin liposomes protect against radiation‑induced pulmonary injury in a murine model

Authors:
- Hao Liu
- Jian‑Xing Xue
- Xing Li
- Rui Ao
- You Lu
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Affiliations: Department of Thoracic Oncology, Cancer Center and State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, P.R. China, Department of Oncology, Sichuan Academy of Medical Sciences, Sichuan Provincial People's Hospital, Chengdu, Sichuan 610041, P.R. China
Published online on: May 29, 2013 https://doi.org/10.3892/ol.2013.1365
Pages: 453-459

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Abstract

In the present study, the hypothesis that quercetin liposomes are able to effectively protect against radiation‑induced pulmonary injury in a murine model was tested. C57BL/6J mice receiving whole‑thorax radiotherapy (16 Gy) were randomly divided into three groups: control, radiation therapy plus saline (RT+NS) and RT plus quercetin (RT+QU). At 1, 4, 8 and 24 weeks post‑irradiation, lung injury was assessed by measuring oxidative damage and the extent of acute pneumonitis and late fibrosis. In the lung tissues from the RT+NS group, the malondialdehyde (MDA) levels were significantly elevated and superoxide dismutase (SOD) and glutathione peroxidase (GSH‑PX) activities were significantly reduced; the total cell counts and inflammatory cell proportions in the bronchoalveolar lavage fluid (BALF), plasma tumor necrosis factor (TNF)‑α and transforming growth factor (TGF)‑β1 concentrations and the hydroxyproline (HP) content were significantly increased. Quercetin liposome administration significantly reduced the MDA content and increased SOD and GSH‑PX activities in the lung tissues, and reduced the total cell counts and inflammatory cell proportions in the BALF, plasma TNF‑α and TGF‑β1 concentrations and the HP content in the lung tissues. A histological examination revealed suppression of the inflammatory response and reduced TGF‑β1 expression and fibrosis scores. Radiation‑induced oxidative damage ranged from pneumonitis to lung fibrosis. Quercetin liposomes were shown to protect against radiation‑induced acute pneumonitis and late fibrosis, potentially by reducing oxidative damage.

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