search button

ER‑α36‑mediated gastric cancer cell proliferation via the c‑Src pathway

  • Authors:
    • Xuming Wang
    • Hao Deng
    • Feng Zou
    • Zhenqi Fu
    • Ying  Chen
    • Zhaoyi Wang
    • Lijiang Liu

  • View Affiliations

  • Published online on: June 20, 2013     https://doi.org/10.3892/ol.2013.1416
  • Pages: 329-335
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Previously, a novel variant of estrogen receptor (ER)‑α, ER‑α36, was identified and cloned and reported to mainly mediate non‑genomic estrogen signaling. More recently, we identified that ER‑α36 is important for the invasion and lymph node metastasis of human gastric cancer. In the present study, the c‑Src signaling pathway was demonstrated to be involved in the non‑genomic estrogen signaling mediated by ER‑α36 in SGC7901 gastric cancer cells. SGC7901 cells were subjected to the siRNA‑mediated knockdown of ER‑α36 (PLKO.1‑PURO‑SP6‑ER‑α36‑L) or transfected with an ER‑α36 upregulated expression plasmid (PLJM1‑ER‑α36‑H) and treated with 17β‑estradiol (E2β) and PP2, a c‑Src protein inhibitor. The expression of ER‑α36 and c‑src/p‑c‑Src and cyclin D1 was examined by western blot analysis, and tumor cell growth was analyzed by cell proliferation and nude mouse xenograft assays. The ER variant, ER‑α36, was shown to enhance gastric cancer cell proliferation through activation of the membrane‑initiated c‑Src signaling pathways, indicating that ER‑α36 is important for the regulation of proliferation in gastric cancer. In addition, ER‑α36 was shown to directly interact with c‑Src by immunoprecipitation. The results of the present study indicate that the use of ER‑α36 may be a targeted therapeutic approach in gastric cancer.
View Figures
View References

Related Articles

Journal Cover

August 2013
Volume 6 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

  • Journal Metrics
  • Impact Factor: 2.9
  • Ranked Oncology
    (total number of cites)
  • CiteScore: 6.3
  • CiteScore Rank: Q2: #119/366 Oncology
  • Source Normalized Impact per Paper (SNIP): 0.725
  • SCImago Journal Rank (SJR): 0.639
International Symposium on Molecular Medicine Conference Banner
The Cancer Story
World Congress on Advances in Oncology and International Symposium on Molecular Medicine Conference Banner
World Academy Of Sciences Banner
Heyday Road Banner

0

Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Deng H, Zou F, Fu Z, Chen Y, Wang Z and Liu L: ER‑α36‑mediated gastric cancer cell proliferation via the c‑Src pathway. Oncol Lett 6: 329-335, 2013
APA
Wang, X., Deng, H., Zou, F., Fu, Z., Chen, Y., Wang, Z., & Liu, L. (2013). ER‑α36‑mediated gastric cancer cell proliferation via the c‑Src pathway. Oncology Letters, 6, 329-335. https://doi.org/10.3892/ol.2013.1416
MLA
Wang, X., Deng, H., Zou, F., Fu, Z., Chen, Y., Wang, Z., Liu, L."ER‑α36‑mediated gastric cancer cell proliferation via the c‑Src pathway". Oncology Letters 6.2 (2013): 329-335.
Chicago
Wang, X., Deng, H., Zou, F., Fu, Z., Chen, Y., Wang, Z., Liu, L."ER‑α36‑mediated gastric cancer cell proliferation via the c‑Src pathway". Oncology Letters 6, no. 2 (2013): 329-335. https://doi.org/10.3892/ol.2013.1416