Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
September 2013 Volume 6 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
September 2013 Volume 6 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Effects of gamma irradiation on cell cycle, apoptosis and telomerase activity in p53 wild‑type and deficient HCT116 colon cancer cell lines

  • Authors:
    • Sevil Oskay Halacli
    • Hande Canpinar
    • Eren Cimen
    • Asuman Sunguroglu
  • View Affiliations / Copyright

    Affiliations: Pediatric Immunology Unit, Institute of Children's Health, Hacettepe University, Sihhiye, Ankara 06100, Turkey, Department of Basic Oncology, Institute of Oncology, Hacettepe University, Sihhiye, Ankara 06100, Turkey, Pediatric Hematology Unit, Institute of Children's Health, Hacettepe University, Sihhiye, Ankara 06100, Turkey, Department of Medical Biology, Faculty of Medicine, Ankara University, Sihhiye, Ankara 06100, Turkey
  • Pages: 807-810
    |
    Published online on: July 3, 2013
       https://doi.org/10.3892/ol.2013.1441
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Radiotherapy serves as adjunctive treatment to chemotherapy and surgical resection of colorectal cancer. However, the cellular response to irradiation varies depending on the expression of tumor suppressor p53, which plays a significant role in the regulation of cell cycle arrest, apoptosis and telomerase activity in various cancers. The present study aimed to investigate cell cycle arrest, apoptosis and telomerase activity with respect to p53 expression in p53 wild‑type (+/+) and deficient (-/-) HCT116 colon cancer cell lines following 5 Gy γ‑irradiation. Cell cycle arrest and apoptosis were evaluated using flow cytometry. The telomerase activity was measured using a TRAP (telomerase repeat amplification protocol) assay. Following treatment with irradiation, G1/S cell cycle arrest occurred in the p53+/+ cells, whereas the p53-/- cells accumulated in the G2 phase. No differences were observed in the apoptotic ratios between the two cell lines following irradiation. Decreased telomerase activity was observed in the p53+/+ cells, whereas telomerase activity was increased in the p53-/- cells. The results showed that while telomerase activity and G1 cell cycle arrest were regulated depending on the p53 status, G2 arrest and the apoptotic response were promoted via a p53‑independent pathway.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Fearon ER and Vogelstein B: A genetic model for colorectal tumorigenesis. Cell. 61:759–767. 1990. View Article : Google Scholar : PubMed/NCBI

2 

Caspari T: How to activate p53. Curr Biol. 10:R315–R317. 2000. View Article : Google Scholar

3 

Brooks CL and Gu W: Ubiquitination, phosphorylation and acetylation: the molecular basis for p53 regulation. Curr Opin Cell Biol. 15:164–171. 2003. View Article : Google Scholar : PubMed/NCBI

4 

Liang SH and Clarke MF: Regulation of p53 localization. Eur J Biochem. 268:2779–2783. 2001. View Article : Google Scholar : PubMed/NCBI

5 

Adimoolam S and Ford JM: p53 regulation of DNA damage recognition during nucleotide excision repair. DNA Repair (Amst). 2:947–954. 2003. View Article : Google Scholar : PubMed/NCBI

6 

Appella E and Anderson CW: Post-translational modifications and activation of p53 by genotoxic stresses. Eur J Biochem. 268:2764–2772. 2001. View Article : Google Scholar : PubMed/NCBI

7 

Attardi LD, de Vries A and Jacks T: Activation of the p53-dependent G1 checkpoint response in mouse embryo fibroblasts depends on the specific DNA damage inducer. Oncogene. 23:973–980. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Donzelly M and Draetta GF: Regulating mammalian checkpoints through Cdc25 inactivation. EMBO Rep. 4:671–677. 2003. View Article : Google Scholar : PubMed/NCBI

9 

Hermeking H, Lengauer C, Polyak K, He TC, Zhang L, Thiagalingam S, Kinzler KW and Vogelstein B: 14-3-3 sigma is a p53-regulated inhibitor of G2/M progression. Mol Cell. 1:3–11. 1997. View Article : Google Scholar

10 

Dou PQ, An B and Will PL: Induction of a retinoblastoma phosphatase activity by anticancer drugs accompanies p53-independent G1 arrest and apoptosis. Proc Natl Acad Sci USA. 92:9019–9023. 1995. View Article : Google Scholar : PubMed/NCBI

11 

Fei P and El-Deiry WS: P53 and radiation responses. Oncogene. 22:5774–5783. 2003. View Article : Google Scholar : PubMed/NCBI

12 

Attardi LD: The role of p53-mediated apoptosis as a crucial anti-tumor response to genomic instability: lessons from mouse models. Mutat Res. 569:145–157. 2005. View Article : Google Scholar : PubMed/NCBI

13 

Enns L, Bogen KT, Wizniak J, Murtha AD and Weinfeld M: Low-dose radiation hypersensitivity is associated with p53-dependent apoptosis. Mol Cancer Res. 2:557–566. 2004.PubMed/NCBI

14 

Hussain SP and Harris CC: p53 biological network: at the crossroads of the cellular-stress response pathway and molecular carcinogenesis. J Nippon Med Sch. 73:54–64. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Speidel D, Helmbold H and Deppert W: Dissection of transcriptional and non-transcriptional p53 activities in the response to genotoxic stress. Oncogene. 25:940–953. 2006. View Article : Google Scholar : PubMed/NCBI

16 

Vávrová J, Rezácová M, Vokurková D and Psutka J: Cell cycle alteration, apoptosis and response of leukemic cell lines to gamma radiation with high- and low-dose rate. Physiol Res. 53:335–342. 2004.PubMed/NCBI

17 

Vousden KH: p53: death star. Cell. 103:691–694. 2000. View Article : Google Scholar : PubMed/NCBI

18 

Kusumoto M, Ogawa T, Mizumoto K, Ueno H, Niiyama H, Sato N, Nakamura M and Tanaka M: Adenovirus-mediated p53 gene transduction inhibits telomerase activity independent of its effects on cell cycle arrest and apoptosis in human pancreatic cancer cells. Clin Cancer Res. 5:2140–2147. 1999.

19 

Shats I, Milyavsky M, Tang X, Stambolsky P, Erez N, Brosh R, Kogan I, Braunstein I, Tzukerman M, Ginsberg D and Rotter V: p53-dependent down-regulation of telomerase is mediated by p21waf1. J Biol Chem. 279:50976–50985. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Myllyperkiö MH, Koski TR, Vilpo LM and Vilpo JA: Gammairradiation-induced DNA single- and double-strand breaks and their repair in chronic lymphocytic leukemia cells of variable radiosensitivity. Hematol Cell Ther. 41:95–103. 1999.PubMed/NCBI

21 

Neuhof D, Ruess A, Wenz F and Weber KJ: Induction of telomerase activity by irradiation in human lymphoblasts. Radiat Res. 155:693–697. 2001. View Article : Google Scholar : PubMed/NCBI

22 

Mailand N, Podtelejnikov AV, Groth A, Mann M, Bartek J and Lukas J: Regulation of G(2)/M events by Cdc25A through phosphorylation-dependent modulation of its stability. EMBO J. 21:5911–5920. 2002. View Article : Google Scholar : PubMed/NCBI

23 

Wichmann A, Jaklevic B and Su TT: Ionising radiation induces caspase-dependent but Chk2-and p53-independent cell death in Drosophila melanogaster. Proc Natl Acad Sci USA. 103:9952–9957. 2006. View Article : Google Scholar : PubMed/NCBI

24 

Cao Y, Li H, Deb S and Liu JP: TERT regulates cell survival independent of telomerase enzymatic activity. Oncogene. 21:3130–3138. 2002. View Article : Google Scholar : PubMed/NCBI

25 

Harada K, Arita K, Kurisu K and Tahara H: Telomerase activity and the expression of telomerase components in pituitary adenoma with malignant transformation. Surg Neurol. 53:267–274. 2000. View Article : Google Scholar : PubMed/NCBI

26 

Jones KR, Elmore LW, Jackson-Cook C, Demasters G, Povirk LF, Holt SE and Gewirtz DA: p53-Dependent accelerated senescence induced by ionising radiation in breast tumour cells. Int J Radiat Biol. 81:445–458. 2005. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Halacli SO, Canpinar H, Cimen E and Sunguroglu A: Effects of gamma irradiation on cell cycle, apoptosis and telomerase activity in p53 wild‑type and deficient HCT116 colon cancer cell lines. Oncol Lett 6: 807-810, 2013.
APA
Halacli, S.O., Canpinar, H., Cimen, E., & Sunguroglu, A. (2013). Effects of gamma irradiation on cell cycle, apoptosis and telomerase activity in p53 wild‑type and deficient HCT116 colon cancer cell lines. Oncology Letters, 6, 807-810. https://doi.org/10.3892/ol.2013.1441
MLA
Halacli, S. O., Canpinar, H., Cimen, E., Sunguroglu, A."Effects of gamma irradiation on cell cycle, apoptosis and telomerase activity in p53 wild‑type and deficient HCT116 colon cancer cell lines". Oncology Letters 6.3 (2013): 807-810.
Chicago
Halacli, S. O., Canpinar, H., Cimen, E., Sunguroglu, A."Effects of gamma irradiation on cell cycle, apoptosis and telomerase activity in p53 wild‑type and deficient HCT116 colon cancer cell lines". Oncology Letters 6, no. 3 (2013): 807-810. https://doi.org/10.3892/ol.2013.1441
Copy and paste a formatted citation
x
Spandidos Publications style
Halacli SO, Canpinar H, Cimen E and Sunguroglu A: Effects of gamma irradiation on cell cycle, apoptosis and telomerase activity in p53 wild‑type and deficient HCT116 colon cancer cell lines. Oncol Lett 6: 807-810, 2013.
APA
Halacli, S.O., Canpinar, H., Cimen, E., & Sunguroglu, A. (2013). Effects of gamma irradiation on cell cycle, apoptosis and telomerase activity in p53 wild‑type and deficient HCT116 colon cancer cell lines. Oncology Letters, 6, 807-810. https://doi.org/10.3892/ol.2013.1441
MLA
Halacli, S. O., Canpinar, H., Cimen, E., Sunguroglu, A."Effects of gamma irradiation on cell cycle, apoptosis and telomerase activity in p53 wild‑type and deficient HCT116 colon cancer cell lines". Oncology Letters 6.3 (2013): 807-810.
Chicago
Halacli, S. O., Canpinar, H., Cimen, E., Sunguroglu, A."Effects of gamma irradiation on cell cycle, apoptosis and telomerase activity in p53 wild‑type and deficient HCT116 colon cancer cell lines". Oncology Letters 6, no. 3 (2013): 807-810. https://doi.org/10.3892/ol.2013.1441
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team