Sinomenine sensitizes gastric cancer cells to 5-fluorouracil in vitro and in vivo

  • Authors:
    • Fei Liao
    • Zirong Yang
    • Xiaohong Lu
    • Xufeng Guo
    • Weiguo Dong
  • View Affiliations

  • Published online on: September 18, 2013     https://doi.org/10.3892/ol.2013.1592
  • Pages: 1604-1610
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Abstract

Sinomenine (SIN) has been reported to exert antitumor effects in various types of human cancer. The present study aimed to investigate the effects of SIN on gastric cancer and to briefly address its mechanism of action. In this study, the single and combined effects of SIN with 5‑fluorouracil (5‑FU) on human gastric cancer cells were assessed using an MTT assay, a combination index method and an MKN‑28 xenograft mice model. Levels of apoptosis were determined using Hoechst 33258 staining and flow cytometry. Expression levels of certain apoptosis‑related proteins were examined by western blotting. mRNA levels of the 5‑FU‑associated gene, thymidylate synthase (TS), were measured by RT‑PCR. The results showed that SIN enhances 5‑FU‑mediated cellular growth inhibition and apoptosis in gastric cancer cells, reduces TS mRNA accumulation and activates the mitochondrial apoptotic pathway. The same chemotherapy sensitizer effect of SIN was confirmed in vivo. SIN is a promising chemotherapy sensitizer for 5‑FU. Our results indicate that this may be a potential combination chemotherapeutic strategy for gastric cancer.
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December 2013
Volume 6 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Liao F, Yang Z, Lu X, Guo X and Dong W: Sinomenine sensitizes gastric cancer cells to 5-fluorouracil in vitro and in vivo. Oncol Lett 6: 1604-1610, 2013
APA
Liao, F., Yang, Z., Lu, X., Guo, X., & Dong, W. (2013). Sinomenine sensitizes gastric cancer cells to 5-fluorouracil in vitro and in vivo. Oncology Letters, 6, 1604-1610. https://doi.org/10.3892/ol.2013.1592
MLA
Liao, F., Yang, Z., Lu, X., Guo, X., Dong, W."Sinomenine sensitizes gastric cancer cells to 5-fluorouracil in vitro and in vivo". Oncology Letters 6.6 (2013): 1604-1610.
Chicago
Liao, F., Yang, Z., Lu, X., Guo, X., Dong, W."Sinomenine sensitizes gastric cancer cells to 5-fluorouracil in vitro and in vivo". Oncology Letters 6, no. 6 (2013): 1604-1610. https://doi.org/10.3892/ol.2013.1592