DNA methylation as an epigenetic biomarker in colorectal cancer

  • Authors:
    • Tiago Donizetti Silva
    • Veronica Marques Vidigal
    • Aledson Vitor Felipe
    • Jacqueline Miranda De Lima
    • Ricardo Artigiani Neto
    • Sarhan Sidney Saad
    • Nora Manoukian Forones
  • View Affiliations

  • Published online on: October 7, 2013     https://doi.org/10.3892/ol.2013.1606
  • Pages: 1687-1692
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Abstract

Sporadic colorectal cancer (CRC) is a consequence of the accumulation of genetic and epigenetic alterations that result in the transformation of normal colonic epithelial cells to adenocarcinomas. Studies have indicated that a common event in the tumorigenesis of CRC is the association of global hypomethylation with discrete hypermethylation at the promoter regions of specific genes that are involved in cell cycle regulation, DNA repair, apoptosis, angiogenesis, adhesion and invasion. The present study aimed to investigate the epigenetic changes (DNA methylation) in 24 candidate genes in CRC. A total of 10 candidate hypermethylated (HM) and unmethylated (UM) genes were identified that may be useful epigenetic markers for non‑invasive CRC screening. The five genes that had the highest average UM percentages in the control group were MLH1 (71.7%), DKK2 (69.6%), CDKN2A (68.4%), APC (67.5%) and hsa‑mir‑342 (67.4%). RUNX3 (58.9%), PCDH10 (55.5%), SFRP5 (52.1%), IGF2 (50.4%) and Hnf1b (50.0%) were the five genes with the highest average HM percentages in the test group. In summary, the present preliminary study identified the methylation profiles of normal and cancerous colonic epithelial tissues, and provided the groundwork for future large‑scale methylation studies.
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December 2013
Volume 6 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Silva TD, Vidigal VM, Felipe AV, De Lima JM, Neto RA, Saad SS and Forones NM: DNA methylation as an epigenetic biomarker in colorectal cancer. Oncol Lett 6: 1687-1692, 2013
APA
Silva, T.D., Vidigal, V.M., Felipe, A.V., De Lima, J.M., Neto, R.A., Saad, S.S., & Forones, N.M. (2013). DNA methylation as an epigenetic biomarker in colorectal cancer. Oncology Letters, 6, 1687-1692. https://doi.org/10.3892/ol.2013.1606
MLA
Silva, T. D., Vidigal, V. M., Felipe, A. V., De Lima, J. M., Neto, R. A., Saad, S. S., Forones, N. M."DNA methylation as an epigenetic biomarker in colorectal cancer". Oncology Letters 6.6 (2013): 1687-1692.
Chicago
Silva, T. D., Vidigal, V. M., Felipe, A. V., De Lima, J. M., Neto, R. A., Saad, S. S., Forones, N. M."DNA methylation as an epigenetic biomarker in colorectal cancer". Oncology Letters 6, no. 6 (2013): 1687-1692. https://doi.org/10.3892/ol.2013.1606