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Article

Apoptosis induced by β,β‑dimethylacrylshikonin is associated with Bcl‑2 and NF‑κB in human breast carcinoma MCF‑7 cells

  • Authors:
    • Yao Xiong
    • Xiu‑Ying Ma
    • Ziran Zhang
    • Zhen‑Jun Shao
    • Yuan‑Yuan Zhang
    • Li‑Ming Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology, Preclinical and Forensic Medical College, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Hongjitang Pharmaceutical Co., Ltd., Jinan, Shandong 250000, P.R. China
  • Pages: 1789-1793
    |
    Published online on: October 10, 2013
       https://doi.org/10.3892/ol.2013.1613
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Abstract

β,β‑dimethylacrylshikonin (DA) is a natural naphthoquinone derivative compound of Lithospermum erythrorhizon with various biological activities. The present study aimed to investigate the inhibitory effects and underlying mechanisms of DA in human breast carcinoma MCF‑7 cells. The 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide (MTT) assay showed that DA inhibited the proliferation of MCF‑7 cells in a dose‑ and time‑dependent manner. The half maximal inhibitory concentration of DA with regard to the proliferation of MCF‑7 cells was 0.050±0.016 mM. The characteristics of cell apoptosis, including cell shrinkage, nuclear pyknosis and chromatin condensation, were all observed in DA‑treated cells. DA decreased the expression levels of Bcl‑2 and increased the expression of Bax and caspase‑3 compared with those in the control. DA inhibited the activity of the nuclear factor (NF)‑κB pathway, by downregulating the expression of the p65 subunit, and inhibited the Iκb phosphorylation. DA inhibits the proliferation of MCF‑7 cells in vitro by inducing apoptosis through the downregulation of Bcl‑2, upregulation of Bax and partial inactivation of the NF‑κB pathway.
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1 

Jemal A, Bray F, Center MM, et al: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar

2 

Siegel R, Naishadham D and Jemal A: Cancer statistics, 2012. CA Cancer J Clin. 62:10–29. 2012. View Article : Google Scholar

3 

Hortobagyi GN: Treatment of breast cancer. N Engl J Med. 339:974–984. 1998. View Article : Google Scholar

4 

Chen X, Yang L, Zhang N, et al: Shikonin, a component of Chinese herbal medicine, inhibits chemokine receptor function and suppresses human immunodeficiency virus type 1. Antimicrob Agents Chemother. 47:2810–2816. 2003. View Article : Google Scholar : PubMed/NCBI

5 

Mao X, Yu CR, Li WH and Li WX: Induction of apoptosis by shikonin through a ROS/JNK-mediated process in Bcr/Abl-positive chronic myelogenous leukemia (CML) cells. Cell Res. 18:879–888. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Yang H, Zhou P, Huang H, et al: Shikonin exerts antitumor activity via proteasome inhibition and cell death induction in vitro and in vivo. Int J Cancer. 124:2450–2459. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Wu YY, Wan LH, Zheng XW, et al: Inhibitory effects of β,β-dimethylacrylshikonin on hepatocellular carcinoma in vitro and in vivo. Phytother Res. 26:764–771. 2012.

8 

Pietrosiuk A, Furmanowa M, Skopińiska-Rózewska E, et al: The effect of acetylshikonin isolated from Lithospermum canescens roots on tumor-induced cutaneous angiogenesis. Acta Pol Pharm. 61:379–382. 2004.

9 

Gong K and Li W: Shikonin, a Chinese plant-derived naphthoquinone, induces apoptosis in hepatocellular carcinoma cells through reactive oxygen species: A potential new treatment for hepatocellular carcinoma. Free Radic Biol Med. 51:2259–2271. 2011. View Article : Google Scholar

10 

Kim SH, Kang IC, Yoon TJ, et al: Antitumor activities of a newly synthesized shikonin derivative, 2-hyim-DMNQ-S-33. Cancer Lett. 172:171–175. 2001. View Article : Google Scholar : PubMed/NCBI

11 

Litchfield JT Jr and Wilcoxon F: A simplified method of evaluating dose-effect experiments. J Pharmacol Exp Ther. 96:99–113. 1949.PubMed/NCBI

12 

Zeng Y, Liu G and Zhou LM: Inhibitory effect of acetylshikonin on human gastric carcinoma cell line SGC-7901 in vitro and in vivo. World J Gastroenterol. 15:1816–1820. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Chen X, Yang L, Oppenheim JJ and Howard MZ: Cellular pharmacology studies of shikonin derivatives. Phytother Res. 16:199–209. 2002. View Article : Google Scholar

14 

Lu L, Qin A, Huang H, et al: Shikonin extracted from medicinal Chinese herbs exerts anti-inflammatory effect via proteasome inhibition. Eur J Pharmacol. 658:242–247. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Sankawa U, Ebizuka Y, Miyazaki T, et al: Antitumor activity of shikonin and its derivatives. Chem Pharm Bull (Tokyo). 25:2392–2395. 1977. View Article : Google Scholar

16 

Yoshimi N, Wang A, Morishita Y, et al: Modifying effects of fungal and herb metabolites on azoxymethane-induced intestinal carcinogenesis in rats. Jpn J Cancer Res. 83:1273–1278. 1992. View Article : Google Scholar : PubMed/NCBI

17 

Ahn BZ, Baik KU, Kweon GR, et al: Acylshikonin analogues: synthesis and inhibition of DNA topoisomerase-I. J Med Chem. 38:1044–1047. 1995. View Article : Google Scholar : PubMed/NCBI

18 

Plyta ZF, Li T, Papageorgiou VP, et al: Inhibition of topoisomerase I by naphthoquinone derivatives. Bioorg Med Chem Lett. 8:3385–3390. 1998. View Article : Google Scholar : PubMed/NCBI

19 

Yang F, Chen Y, Duan W, et al: SH-7, a new synthesized shikonin derivative, exerting its potent antitumor activities as a topoisomerase inhibitor. Int J Cancer. 119:1184–1193. 2006. View Article : Google Scholar : PubMed/NCBI

20 

Gaddipati JP, Mani H, Shefali, et al: Inhibition of growth and regulation of IGFs and VEGF in human prostate cancer cell lines by shikonin analogue 93/637 (SA). Anticancer Res. 20:2547–2552. 2000.PubMed/NCBI

21 

Chang IC, Huang YJ, Chiang TI, et al: Shikonin induces apoptosis through reactive oxygen species/extracellular signal-regulated kinase pathway in osteosarcoma cells. Biol Pharm Bull. 33:816–824. 2010. View Article : Google Scholar

22 

Zhen-Jun S, Yuan-Yuan Z, Ying-Ying F, et al: β,β-Dimethylacrylshikonin exerts antitumor activity via Notch-1 signaling pathway in vitro and in vivo. Biochem Pharmacol. 84:507–512. 2012.

23 

Green DR and Evan GI: A matter of life and death. Cancer Cell. 1:19–30. 2002. View Article : Google Scholar

24 

Adams JM and Cory S: The Bcl-2 protein family: arbiters of cell survival. Science. 281:1322–1326. 1998. View Article : Google Scholar : PubMed/NCBI

25 

Oltvai ZN, Milliman CL and Korsmeyer SJ: Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell. 74:609–619. 1993. View Article : Google Scholar : PubMed/NCBI

26 

Shen HM and Tergaonkar V: NFκB signaling in carcinogenesis and as a potential molecular target for cancer therapy. Apoptosis. 14:348–363. 2009.

27 

Tergaonkar V, Correa RG, Ikawa M and Verma IM: Distinct roles of IκB proteins in regulating constitutive NF-κB activity. Nat Cell Biol. 7:921–923. 2005.

28 

Basak S, Kim H, Kearns JD, et al: A fourth IκB protein within the NF-κB signaling module. Cell. 128:369–381. 2007.

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Copy and paste a formatted citation
Spandidos Publications style
Xiong Y, Ma XY, Zhang Z, Shao ZJ, Zhang YY and Zhou LM: Apoptosis induced by β,β‑dimethylacrylshikonin is associated with Bcl‑2 and NF‑κB in human breast carcinoma MCF‑7 cells. Oncol Lett 6: 1789-1793, 2013.
APA
Xiong, Y., Ma, X., Zhang, Z., Shao, Z., Zhang, Y., & Zhou, L. (2013). Apoptosis induced by β,β‑dimethylacrylshikonin is associated with Bcl‑2 and NF‑κB in human breast carcinoma MCF‑7 cells. Oncology Letters, 6, 1789-1793. https://doi.org/10.3892/ol.2013.1613
MLA
Xiong, Y., Ma, X., Zhang, Z., Shao, Z., Zhang, Y., Zhou, L."Apoptosis induced by β,β‑dimethylacrylshikonin is associated with Bcl‑2 and NF‑κB in human breast carcinoma MCF‑7 cells". Oncology Letters 6.6 (2013): 1789-1793.
Chicago
Xiong, Y., Ma, X., Zhang, Z., Shao, Z., Zhang, Y., Zhou, L."Apoptosis induced by β,β‑dimethylacrylshikonin is associated with Bcl‑2 and NF‑κB in human breast carcinoma MCF‑7 cells". Oncology Letters 6, no. 6 (2013): 1789-1793. https://doi.org/10.3892/ol.2013.1613
Copy and paste a formatted citation
x
Spandidos Publications style
Xiong Y, Ma XY, Zhang Z, Shao ZJ, Zhang YY and Zhou LM: Apoptosis induced by β,β‑dimethylacrylshikonin is associated with Bcl‑2 and NF‑κB in human breast carcinoma MCF‑7 cells. Oncol Lett 6: 1789-1793, 2013.
APA
Xiong, Y., Ma, X., Zhang, Z., Shao, Z., Zhang, Y., & Zhou, L. (2013). Apoptosis induced by β,β‑dimethylacrylshikonin is associated with Bcl‑2 and NF‑κB in human breast carcinoma MCF‑7 cells. Oncology Letters, 6, 1789-1793. https://doi.org/10.3892/ol.2013.1613
MLA
Xiong, Y., Ma, X., Zhang, Z., Shao, Z., Zhang, Y., Zhou, L."Apoptosis induced by β,β‑dimethylacrylshikonin is associated with Bcl‑2 and NF‑κB in human breast carcinoma MCF‑7 cells". Oncology Letters 6.6 (2013): 1789-1793.
Chicago
Xiong, Y., Ma, X., Zhang, Z., Shao, Z., Zhang, Y., Zhou, L."Apoptosis induced by β,β‑dimethylacrylshikonin is associated with Bcl‑2 and NF‑κB in human breast carcinoma MCF‑7 cells". Oncology Letters 6, no. 6 (2013): 1789-1793. https://doi.org/10.3892/ol.2013.1613
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