Role of microRNA‑133a in epithelial ovarian cancer pathogenesis and progression

Luo,Jie; Zhou,Jianhong; Cheng,Qi; Zhou,Caiyun; Ding,Zhiming

doi:10.3892/ol.2014.1841

Role of microRNA‑133a in epithelial ovarian cancer pathogenesis and progression

Authors:
- Jie Luo
- Jianhong Zhou
- Qi Cheng
- Caiyun Zhou
- Zhiming Ding
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Affiliations: Department of Gynecologic Oncology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310006, P.R. China
Published online on: January 29, 2014 https://doi.org/10.3892/ol.2014.1841
Pages: 1043-1048

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Abstract

It has been demonstrated that microRNA (miR)‑133a is downregulated in a number of human malignancies and is closely associated with the progression of tumors. The present study was conducted to investigate the contribution of miR‑133a to the initiation and malignant progression of human epithelial ovarian cancer (EOC). Quantitative polymerase chain reaction was employed to detect the expression of miR‑133a in the human EOC OVCAR‑3 cell line, normal human ovarian surface epithelial (tsT) cells and 96 tissue samples, including 70 EOC tissues and 26 normal ovarian tissue sections. Additionally, analysis of the correlation between miR‑133a levels and clinicopathological characteristics was carried out. The effect of miR‑133a on cell viability, apoptosis, invasion and migration was investigated following transfection with miR‑133a mimics and negative control small interfering RNA in OVCAR‑3 cells. Marked downregulation of miR‑133a was observed in the OVCAR‑3 cell line and primary tumor samples, and it was found that reduced miR‑133a expression significantly correlated with advanced clinical stages, poor histological differentiation and lymph node metastasis. Furthermore, OVCAR‑3 cell viability, invasion and migration were significantly inhibited, while cell apoptosis was increased, following transfection of miR‑133a mimics. The present study reveals the critical role that miR‑133a plays in EOC pathogenesis and development, indicating that it may act as a promising biomarker for predicting EOC progression and as a potential target for gene therapy.

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