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Article

Inhibition of microRNA‑21 upregulates the expression of programmed cell death 4 and phosphatase tensin homologue in the A431 squamous cell carcinoma cell line

  • Authors:
    • Xiaohong Li
    • Kai Huang
    • Jianbin Yu
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, Department of Oncology, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan 450052, P.R. China
  • Pages: 203-207
    |
    Published online on: April 15, 2014
       https://doi.org/10.3892/ol.2014.2066
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Abstract

microRNA‑21 (miRNA/miR‑21) is a well‑known oncogenic miRNA that is overexpressed in various carcinomas. The tumor suppressor genes, programmed cell death 4 (PDCD4) and phosphatase tensin homologue (PTEN), which target miR‑21, are underexpressed in several types of cancer. However, the expression of miR‑21 and its target genes, PDCD4 and PTEN, has not yet been reported in skin squamous cell carcinoma (SCC). In the present study, anti‑miR‑21 was transfected into the A431 cell line, and the expression of miR‑21, PDCD4 and PTEN and the level of cell apoptosis were detected by quantitative polymerase chain reaction, immunocytochemistry and western blotting, and terminal deoxynucleotidyl transferase‑mediated dUTP nick end labeling, respectively. The expression levels of PDCD4 and PTEN in the A431 cell line transfected with anti‑miR‑21 were significantly increased (P<0.05) and the apoptotic ratio was significantly increased (P<0.05). The data indicate that miR‑21 may play an oncogenic role in the cellular processes of SCC and represent a novel target for effective therapies.
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Copy and paste a formatted citation
Spandidos Publications style
Li X, Huang K and Yu J: Inhibition of microRNA‑21 upregulates the expression of programmed cell death 4 and phosphatase tensin homologue in the A431 squamous cell carcinoma cell line. Oncol Lett 8: 203-207, 2014.
APA
Li, X., Huang, K., & Yu, J. (2014). Inhibition of microRNA‑21 upregulates the expression of programmed cell death 4 and phosphatase tensin homologue in the A431 squamous cell carcinoma cell line. Oncology Letters, 8, 203-207. https://doi.org/10.3892/ol.2014.2066
MLA
Li, X., Huang, K., Yu, J."Inhibition of microRNA‑21 upregulates the expression of programmed cell death 4 and phosphatase tensin homologue in the A431 squamous cell carcinoma cell line". Oncology Letters 8.1 (2014): 203-207.
Chicago
Li, X., Huang, K., Yu, J."Inhibition of microRNA‑21 upregulates the expression of programmed cell death 4 and phosphatase tensin homologue in the A431 squamous cell carcinoma cell line". Oncology Letters 8, no. 1 (2014): 203-207. https://doi.org/10.3892/ol.2014.2066
Copy and paste a formatted citation
x
Spandidos Publications style
Li X, Huang K and Yu J: Inhibition of microRNA‑21 upregulates the expression of programmed cell death 4 and phosphatase tensin homologue in the A431 squamous cell carcinoma cell line. Oncol Lett 8: 203-207, 2014.
APA
Li, X., Huang, K., & Yu, J. (2014). Inhibition of microRNA‑21 upregulates the expression of programmed cell death 4 and phosphatase tensin homologue in the A431 squamous cell carcinoma cell line. Oncology Letters, 8, 203-207. https://doi.org/10.3892/ol.2014.2066
MLA
Li, X., Huang, K., Yu, J."Inhibition of microRNA‑21 upregulates the expression of programmed cell death 4 and phosphatase tensin homologue in the A431 squamous cell carcinoma cell line". Oncology Letters 8.1 (2014): 203-207.
Chicago
Li, X., Huang, K., Yu, J."Inhibition of microRNA‑21 upregulates the expression of programmed cell death 4 and phosphatase tensin homologue in the A431 squamous cell carcinoma cell line". Oncology Letters 8, no. 1 (2014): 203-207. https://doi.org/10.3892/ol.2014.2066
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