Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
July-2014 Volume 8 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
July-2014 Volume 8 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Voltage‑gated sodium channels were differentially expressed in human normal prostate, benign prostatic hyperplasia and prostate cancer cells

  • Authors:
    • Bin Shan
    • Mei Dong
    • He Tang
    • Na Wang
    • Jin Zhang
    • Changqing Yan
    • Xiaocui Jiao
    • Hailin Zhang
    • Chuan Wang
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China, Department of Surgery, The Affiliated Hospital of Hebei Science and Technology University, Shijiazhuang, Hebei 050018, P.R. China, Department of Urology, The First Hospital of Shijiazhuang, Shijiazhuang, Hebei 050011, P.R. China, Department of Hepatobiliary Surgery, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China
  • Pages: 345-350
    |
    Published online on: May 2, 2014
       https://doi.org/10.3892/ol.2014.2110
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Voltage‑gated sodium channels (VGSCs) are expressed not only in excitable cells but also in numerous metastatic cells, particularly in certain types of cancer cells. In some types of cancer, including prostate cancer, the expression of VGSCs is associated with cancer migration, invasion and metastasis in vivo. However, the detailed expression profiles of VGSC α subunits in normal human prostate, in prostatic hyperplasia and prostatic cancer remain controversial. In the present study, quantitative polymerase chain reaction was used to systematically detect all subtypes of VGSC α subunits in normal human prostate, benign prostatic hyperplasia (BPH) and prostate cancer cells. The expression profile of VGSC α subunits was observed to differ between these cell types. Nav1.5 was the major isoform expressed in normal human prostate tissue, while Nav1.5 and Nav1.2 were the predominant isoforms in BPH tissue. However, in PC‑3 and LNCaP cells, two typical prostate cancer cell lines, Nav1.6 and Nav1.7 were abundantly expressed. By comparing the relative expression levels of Nav1.5, Nav1.6 and Nav1.7 in these cells, the mRNA levels of Nav1.6 and Nav1.7 were identified to be 6- to 27‑fold higher in PC‑3 and LNCaP cells than in either normal or BPH samples (P<0.05); however, Nav1.5 mRNA levels were relatively lower compared with those of Nav1.6 or Nav1.7 in all cells analyzed. To confirm whether Nav1.6 and Nav1.7 expression in cancer cells was functional, a patch‑clamp technique was used to record whole‑cell currents. A tetrodotoxin‑sensitive sodium current was successfully recorded in PC‑3 cells, but not in LNCaP cells. It was concluded that although all types of VGSC α subunits exhibited low expression levels in normal prostate and BPH cells, both Nav1.6 and Nav1.7 were significantly upregulated in the prostate cancer cell lines, suggesting these subtypes may be potential diagnostic markers and therapeutic targets for certain types of prostate cancer in humans.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Catterall WA: The molecular basis of neuronal excitability. Science. 223:653–661. 1984.

2 

Catterall WA: From ionic currents to molecular mechanisms: the structure and function of voltage-gated sodium channels. Neuron. 26:13–25. 2000.

3 

Antzelevitch C, Brugada P, Borggrefe M, et al: Brugada syndrome: report of the second consensus conference: endorsed by the Heart Rhythm Society and the European Heart Rhythm Association. Circulation. 111:659–670. 2005.

4 

Veltmann C, Schimpf R, Echternach C, et al: A prospective study on spontaneous fluctuations between diagnostic and non-diagnostic ECGs in Brugada syndrome: implications for correct phenotyping and risk stratification. Eur Heart J. 27:2544–2552. 2006.

5 

Bennett PB, Yazawa K, Makita N and George AL Jr: Molecular mechanism for an inherited cardiac arrhythmia. Nature. 376:683–685. 1995.

6 

Waxman SG: Painful Na-channelopathies: an expanding universe. Trends Mol Med. 19:406–409

7 

Oliva M, Berkovic SF and Petrou S: Sodium channels and the neurobiology of epilepsy. Epilepsia. 53:1849–1859. 2012.

8 

Brackenbury WJ: Voltage-gated sodium channels and metastatic disease. Channels (Austin). 6:352–361. 2012.

9 

Baade PD, Youlden DR and Krnjacki LJ: International epidemiology of prostate cancer: geographical distribution and secular trends. Mol Nutr Food Res. 53:171–184. 2009.

10 

Diss JK, Stewart D, Pani F, et al: A potential novel marker for human prostate cancer: voltage-gated sodium channel expression in vivo. Prostate Cancer Prostatic Dis. 8:266–273. 2005.

11 

Diss JK, Fraser SP, Walker MM, Patel A, Latchman DS and Djamgoz MB: Beta-subunits of voltage-gated sodium channels in human prostate cancer: quantitative in vitro and in vivo analyses of mRNA expression. Prostate Cancer Prostatic Dis. 11:325–333. 2008.

12 

Laniado ME, Lalani EN, Fraser SP, et al: Expression and functional analysis of voltage-activated Na+ channels in human prostate cancer cell lines and their contribution to invasion in vitro. Am J Pathol. 150:1213–1221. 1997.

13 

Grimes JA, Fraser SP, Stephens GJ, et al: Differential expression of voltage-activated Na+ currents in two prostatic tumour cell lines: contribution to invasiveness in vitro. FEBS Lett. 369:290–294. 1995.

14 

Nguyen TP, Wang DW, Rhodes TH and George AL Jr: Divergent biophysical defects caused by mutant sodium channels in dilated cardiomyopathy with arrhythmia. Circ Res. 102:364–371. 2008.

15 

Benson DW, Wang DW, Dyment M, et al: Congenital sick sinus syndrome caused by recessive mutations in the cardiac sodium channel gene (SCN5A). J Clin Invest. 112:1019–1028. 2003.

16 

Sato PY, Musa H, Coombs W, et al: Loss of plakophilin-2 expression leads to decreased sodium current and slower conduction velocity in cultured cardiac myocytes. Circ Res. 105:523–526. 2009.

17 

Wang C, Hennessey JA, Kirkton RD, et al: Fibroblast growth factor homologous factor 13 regulates Na+ channels and conduction velocity in murine hearts. Circ Res. 109:775–782. 2011.

18 

Nomiya T, Tsuji H, Toyama S, et al: Management of high-risk prostate cancer: radiation therapy and hormonal therapy. Cancer Treat Rev. 39:872–878. 2013.

19 

Diaz D, Delgadillo DM, Hernández-Gallegos E, et al: Functional expression of voltage-gated sodium channels in primary cultures of human cervical cancer. J Cell Physiol. 210:469–478. 2007.

20 

Fraser SP, Diss JK, Chioni AM, et al: Voltage-gated sodium channel expression and potentiation of human breast cancer metastasis. Clin Cancer Res. 11:5381–5389. 2005.

21 

Hernandez-Plata E, Ortiz CS, Marquina-Castillo B, et al: Overexpression of NaV 1.6 channels is associated with the invasion capacity of human cervical cancer. Int J Cancer. 130:2013–2023. 2012.

22 

Roger S, Besson P and Le Guennec JY: Involvement of a novel fast inward sodium current in the invasion capacity of a breast cancer cell line. Biochim Biophys Acta. 1616:107–111. 2003.

23 

Fraser SP, Grimes JA and Djamgoz MB: Effects of voltage-gated ion channel modulators on rat prostatic cancer cell proliferation: comparison of strongly and weakly metastatic cell lines. Prostate. 44:61–76. 2000.

24 

Abdul M and Hoosein N: Voltage-gated sodium ion channels in prostate cancer: expression and activity. Anticancer Res. 22:1727–1730. 2002.

25 

Smith P, Rhodes NP, Shortland AP, et al: Sodium channel protein expression enhances the invasiveness of rat and human prostate cancer cells. FEBS Lett. 423:19–24. 1998.

26 

Brisson L, Gillet L, Calaghan S, et al: Na(V)1.5 enhances breast cancer cell invasiveness by increasing NHE1-dependent H(+) efflux in caveolae. Oncogene. 30:2070–2076. 2011.

27 

Carrithers MD, Chatterjee G, Carrithers LM, et al: Regulation of podosome formation in macrophages by a splice variant of the sodium channel SCN8A. J Biol Chem. 284:8114–8126. 2009.

28 

Diamandis EP and Yu H: Nonprostatic sources of prostate-specific antigen. Urol Clin North Am. 24:275–282. 1997.

29 

Foster CS, Cornford P, Forsyth L, Djamgoz MB and Ke Y: The cellular and molecular basis of prostate cancer. BJU Int. 83:171–194. 1999.

30 

de Kok JB, Verhaegh GW, Roelofs RW, et al: DD3 (PCA3), a very sensitive and specific marker to detect prostate tumors. Cancer Res. 62:2695–2698. 2002.

31 

Chakravarti A and Zhai GG: Molecular and genetic prognostic factors of prostate cancer. World J Urol. 21:265–274. 2003.

32 

Biki B, Mascha E, Moriarty DC, Fitzpatrick JM, Sessler DI and Buggy DJ: Anesthetic technique for radical prostatectomy surgery affects cancer recurrence: a retrospective analysis. Anesthesiology. 109:180–187. 2008.

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Shan B, Dong M, Tang H, Wang N, Zhang J, Yan C, Jiao X, Zhang H and Wang C: Voltage‑gated sodium channels were differentially expressed in human normal prostate, benign prostatic hyperplasia and prostate cancer cells. Oncol Lett 8: 345-350, 2014.
APA
Shan, B., Dong, M., Tang, H., Wang, N., Zhang, J., Yan, C. ... Wang, C. (2014). Voltage‑gated sodium channels were differentially expressed in human normal prostate, benign prostatic hyperplasia and prostate cancer cells. Oncology Letters, 8, 345-350. https://doi.org/10.3892/ol.2014.2110
MLA
Shan, B., Dong, M., Tang, H., Wang, N., Zhang, J., Yan, C., Jiao, X., Zhang, H., Wang, C."Voltage‑gated sodium channels were differentially expressed in human normal prostate, benign prostatic hyperplasia and prostate cancer cells". Oncology Letters 8.1 (2014): 345-350.
Chicago
Shan, B., Dong, M., Tang, H., Wang, N., Zhang, J., Yan, C., Jiao, X., Zhang, H., Wang, C."Voltage‑gated sodium channels were differentially expressed in human normal prostate, benign prostatic hyperplasia and prostate cancer cells". Oncology Letters 8, no. 1 (2014): 345-350. https://doi.org/10.3892/ol.2014.2110
Copy and paste a formatted citation
x
Spandidos Publications style
Shan B, Dong M, Tang H, Wang N, Zhang J, Yan C, Jiao X, Zhang H and Wang C: Voltage‑gated sodium channels were differentially expressed in human normal prostate, benign prostatic hyperplasia and prostate cancer cells. Oncol Lett 8: 345-350, 2014.
APA
Shan, B., Dong, M., Tang, H., Wang, N., Zhang, J., Yan, C. ... Wang, C. (2014). Voltage‑gated sodium channels were differentially expressed in human normal prostate, benign prostatic hyperplasia and prostate cancer cells. Oncology Letters, 8, 345-350. https://doi.org/10.3892/ol.2014.2110
MLA
Shan, B., Dong, M., Tang, H., Wang, N., Zhang, J., Yan, C., Jiao, X., Zhang, H., Wang, C."Voltage‑gated sodium channels were differentially expressed in human normal prostate, benign prostatic hyperplasia and prostate cancer cells". Oncology Letters 8.1 (2014): 345-350.
Chicago
Shan, B., Dong, M., Tang, H., Wang, N., Zhang, J., Yan, C., Jiao, X., Zhang, H., Wang, C."Voltage‑gated sodium channels were differentially expressed in human normal prostate, benign prostatic hyperplasia and prostate cancer cells". Oncology Letters 8, no. 1 (2014): 345-350. https://doi.org/10.3892/ol.2014.2110
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team