Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
July-2014 Volume 8 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
July-2014 Volume 8 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Downregulation of CD147 by chitooligosaccharide inhibits MMP‑2 expression and suppresses the metastatic potential of human gastric cancer

  • Authors:
    • Zhiguo Luo
    • Xiaoxia Dong
    • Qing Ke
    • Qiwen Duan
    • Li Shen
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Oncology, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China, Department of Pharmacology, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China
  • Pages: 361-366
    |
    Published online on: May 7, 2014
       https://doi.org/10.3892/ol.2014.2115
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Metastasis is considered to be the major cause of mortality in patients with cancer, and gastric cancer is a highly metastatic cancer. In the present study, the anti‑metastatic activity of chitooligosaccharide (COS) in human gastric cancer cells and its underlying mechanism were investigated. It was found that COS significantly inhibited SGC‑7901 cell proliferation and metastasis in a dose‑dependent manner, as observed by MTT, wound‑healing and Transwell assays. Quantitative real‑time polymerase chain reaction and western blot analysis indicated that COS could decrease the expression of cluster of differentiation 147 (CD147) and subsequently reduce matrix metalloproteinase‑2 (MMP‑2) expression. A clear dose‑dependent inhibition of MMP‑2 activity was also observed in SGC‑7901 cells following treatment with COS in gelatin zymography experiments. Furthermore, overexpression of CD147 (when transfected with pEGFP‑C1 plasmid) in SGC‑7901 cells partially protected against COS‑induced inhibition of MMP‑2. The results of the present study demonstrated the potential of COS in suppressing gastric cancer metastasis, and that the CD147/MMP‑2 pathway may be involved as the key mechanism of its anti‑metastatic effect.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Zheng L, Qi T, Yang D, et al: microRNA-9 suppresses the proliferation, invasion and metastasis of gastric cancer cells through targeting cyclin D1 and Ets1. PLoS One. 8:e557192013.

2 

Mehlen P and Puisieux A: Metastasis: a question of life or death. Nat Rev Cancer. 6:449–458. 2006.

3 

Nagase H, Visse R and Murphy G: Structure and function of matrix metalloproteinases and TIMPs. Cardiovasc Res. 69:562–573. 2006.

4 

Murray G, Duncan M, Arbuckle E, Melvin W and Fothergill J: Matrix metalloproteinases and their inhibitors in gastric cancer. Gut. 43:791–797. 1998.

5 

Partyka R, Gonciarz M, Jalowiecki P, Kokocinska D and Byrczek T: VEGF and metalloproteinase 2 (MMP 2) expression in gastric cancer tissue. Med Sci Monit. 18:BR130–BR134. 2012.

6 

Zhou J, Zhu P, Jiang JL, et al: Involvement of CD147 in overexpression of MMP-2 and MMP-9 and enhancement of invasive potential of PMA-differentiated THP-1. BMC Cell Biol. 6:252005.

7 

Tang W, Chang SB and Hemler ME: Links between CD147 function, glycosylation, and caveolin-1. Mol Biol Cell. 15:4043–4050. 2004.

8 

Jiang JL, Chan HC, Zhou Q, et al: HAb18G/CD147-mediated calcium mobilization and hepatoma metastasis require both C-terminal and N-terminal domains. Cell Mol Life Sci. 61:2083–2091. 2004.

9 

Voigt H, Vetter-Kauczok CS, Schrama D, Hofmann UB, Becker JC and Houben R: CD147 impacts angiogenesis and metastasis formation. Cancer Invest. 27:329–333. 2009.

10 

Zheng HC, Takahashi H, Murai Y, et al: Upregulated EMMPRIN/CD147 might contribute to growth and angiogenesis of gastric carcinoma: a good marker for local invasion and prognosis. Br J Cancer. 95:1371–1378. 2006.

11 

Wang B, Xu YF, He BS, et al: RNAi-mediated silencing of CD147 inhibits tumor cell proliferation, invasion and increases chemosensitivity to cisplatin in SGC7901 cells in vitro. J Exp Clin Cancer Res. 29:612010.

12 

Li J, He J and Yu C: Chitosan oligosaccharide inhibits LPS-induced apoptosis of vascular endothelial cells through the BKCa channel and the p38 signaling pathway. Int J Mol Med. 30:157–164. 2012.

13 

Lee JK, Lim HS and Kim JH: Cytotoxic activity of aminoderivatized cationic chitosan derivatives. Bioorg Med Chem Lett. 12:2949–2951. 2002.

14 

Chen D, Wang Y, Zhang K, Jiao X, Yan B and Liang J: Antisense oligonucleotide against clusterin regulates human hepatocellular carcinoma invasion through transcriptional regulation of matrix metalloproteinase-2 and e-cadherin. Int J Mol Sci. 13:10594–10607. 2012.

15 

Nam KS and Shon YH: Suppression of metastasis of human breast cancer cells by chitosan oligosaccharides. J Microbiol Biotechnol. 19:629–633. 2009.

16 

Shen KT, Chen MH, Chan HY, Jeng JH and Wang YJ: Inhibitory effects of chitooligosaccharides on tumor growth and metastasis. Food Chem Toxicol. 47:1864–1871. 2009.

17 

Karagozlu MZ, Kim JA, Karadeniz F, Kong CS and Kim SK: Anti-proliferative effect of aminoderivatized chitooligosaccharides on AGS human gastric cancer cells. Process Biochem. 45:1523–1528. 2010.

18 

Rundhaug JE: Matrix metalloproteinases, angiogenesis, and cancer: commentary re: A. C. Lockhart et al., Reduction of wound angiogenesis in patients treated with BMS-275291, a broad spectrum matrix metalloproteinase inhibitor Clin Cancer Res, 9: 00-00, 2003. Clin Cancer Res. 9:551–554. 2003.

19 

Kim MM and Kim SK: Chitooligosaccharides inhibit activation and expression of matrix metalloproteinase-2 in human dermal fibroblasts. FEBS Lett. 580:2661–2666. 2006.

20 

Van Ta Q, Kim MM and Kim SK: Inhibitory effect of chitooligosaccharides on matrix metalloproteinase-9 in human fibrosarcoma cells (HT1080). Mar Biotechnol (NY). 8:593–599. 2006.

21 

Rajapakse N, Kim MM, Mendis E, Huang R and Kim SK: Carboxylated chitooligosaccharides (CCOS) inhibit MMP-9 expression in human fibrosarcoma cells via down-regulation of AP-1. Biochim Biophys Acta. 1760:1780–1788. 2006.

22 

Omi Y, Shibata N, Okamoto T, Obara T and Kobayashi M: The role of CD147 in the invasiveness of follicular thyroid carcinoma cells. Thyroid. 22:383–394. 2012.

23 

Huang W, Luo WJ, Zhu P, et al: Modulation of CD147-induced matrix metalloproteinase activity: role of CD147 N-glycosylation. Biochem J. 449:437–448. 2013.

24 

Li R, Pan Y, He B, et al: Downregulation of CD147 expression by RNA interference inhibits HT29 cell proliferation, invasion and tumorigenicity in vitro and in vivo. Int J Oncol. 43:1885–1894. 2013.

25 

Chen L, Pan Y, Gu L, et al: ERK1/2 signalling pathway is involved in CD147-mediated gastric cancer cell line SGC7901 proliferation and invasion. Exp Biol Med (Maywood). 238:903–912. 2013.

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Luo Z, Dong X, Ke Q, Duan Q and Shen L: Downregulation of CD147 by chitooligosaccharide inhibits MMP‑2 expression and suppresses the metastatic potential of human gastric cancer. Oncol Lett 8: 361-366, 2014.
APA
Luo, Z., Dong, X., Ke, Q., Duan, Q., & Shen, L. (2014). Downregulation of CD147 by chitooligosaccharide inhibits MMP‑2 expression and suppresses the metastatic potential of human gastric cancer. Oncology Letters, 8, 361-366. https://doi.org/10.3892/ol.2014.2115
MLA
Luo, Z., Dong, X., Ke, Q., Duan, Q., Shen, L."Downregulation of CD147 by chitooligosaccharide inhibits MMP‑2 expression and suppresses the metastatic potential of human gastric cancer". Oncology Letters 8.1 (2014): 361-366.
Chicago
Luo, Z., Dong, X., Ke, Q., Duan, Q., Shen, L."Downregulation of CD147 by chitooligosaccharide inhibits MMP‑2 expression and suppresses the metastatic potential of human gastric cancer". Oncology Letters 8, no. 1 (2014): 361-366. https://doi.org/10.3892/ol.2014.2115
Copy and paste a formatted citation
x
Spandidos Publications style
Luo Z, Dong X, Ke Q, Duan Q and Shen L: Downregulation of CD147 by chitooligosaccharide inhibits MMP‑2 expression and suppresses the metastatic potential of human gastric cancer. Oncol Lett 8: 361-366, 2014.
APA
Luo, Z., Dong, X., Ke, Q., Duan, Q., & Shen, L. (2014). Downregulation of CD147 by chitooligosaccharide inhibits MMP‑2 expression and suppresses the metastatic potential of human gastric cancer. Oncology Letters, 8, 361-366. https://doi.org/10.3892/ol.2014.2115
MLA
Luo, Z., Dong, X., Ke, Q., Duan, Q., Shen, L."Downregulation of CD147 by chitooligosaccharide inhibits MMP‑2 expression and suppresses the metastatic potential of human gastric cancer". Oncology Letters 8.1 (2014): 361-366.
Chicago
Luo, Z., Dong, X., Ke, Q., Duan, Q., Shen, L."Downregulation of CD147 by chitooligosaccharide inhibits MMP‑2 expression and suppresses the metastatic potential of human gastric cancer". Oncology Letters 8, no. 1 (2014): 361-366. https://doi.org/10.3892/ol.2014.2115
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team