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Article

Bone marrow mesenchymal stem cell‑derived Wnt5a inhibits leukemia cell progression in vitro via activation of the non‑canonical Wnt signaling pathway

  • Authors:
    • Ya Li Shen
    • Qing Luo
    • Yu Xia Guo
    • Gai Huai Zheng
    • Jie Yu
    • You Hua Xu
  • View Affiliations / Copyright

    Affiliations: Ministry of Education Key Laboratory of Child Development and Disorders, Key Laboratory of Pediatrics in Chongqing, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing 400014, P.R. China, Department of Hematology and Oncology, Children's Hospital of Chongqing Medical University, Chongqing 400014, P.R. China
  • Pages: 85-90
    |
    Published online on: May 7, 2014
       https://doi.org/10.3892/ol.2014.2117
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Abstract

Leukemia is one of the most common malignancies in humans worldwide; however, the molecular mechanism of the effect of bone marrow mesenchymal stem cells (bMSCs) on leukemia cell growth remains unclear. The present study demonstrated that Wnt5a protein expression was significantly induced in bMSCs via an adenovirus vector (P<0.01). The results showed that the proliferation of HL60 cells, a leukemia cell line, was significantly inhibited when the cells were stimulated with the culture supernatant of adeno‑Wnt5a bMSCs compared with the culture supernatants of bMSCs and adeno‑vector bMSCs for 24 or 48 h (P<0.01). The promoted maturation levels of HL60 cells were also observed following stimulation with the culture supernatant of adeno‑Wnt5a bMSCs (P<0.01). However, no significant difference was identified in the proliferation and maturation of HL60 cells among the three groups stimulated with the culture supernatants containing a neutralization antibody against Wnt5a. Furthermore, the bMSC‑derived Wnt5a was found to influence the maturation and proliferation of the HL60 cells by enhancing the non‑canonical Wnt signaling pathway, while inhibiting the canonical Wnt signaling pathway by upregulating the expression of receptor tyrosine kinase‑like orphan receptor 2 and calcium/calmodulin‑dependent protein kinase II, and suppressing the expression of β‑catenin and cyclin D1. In conclusion, bMSC‑derived Wnt5a modifies the proliferation and maturation of HL60 cells via activation of the non‑canonical Wnt signaling pathway.
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1 

Gilliland DG, Jordan CT and Felix CA: The molecular basis of leukemia. Hematology Am Soc Hematol Educ Program. 1:80–97. 2004.

2 

Arai KI, Lee F, Miyajima A, Miyatake S, Arai N and Yokota T: Cytokines: coordinators of immune and inflammatory responses. Annu Rev Biochem. 59:783–836. 1990.

3 

Ploemacher RE, Mayen AE, De Koning AE, et al: Hematopoiesis: gap junction intercellular communication is likely to be involved in regulation of stroma-dependent proliferation of hemopoietic stem cells. Hematology. 5:133–147. 2000.

4 

Reya T and Clevers H: Wnt signalling in stem cells and cancer. Nature. 434:843–850. 2005.

5 

Taketo MM: Shutting down Wnt signal-activated cancer. Nat Genet. 36:320–322. 2004.

6 

McDonald SL and Silver A: The opposing roles of Wnt-5a in cancer. Br J Cancer. 101:209–214. 2009.

7 

Clevers H and Nusse R: Wnt/beta-catenin signaling and disease. Cell. 149:1192–1205. 2012.

8 

Kikuchi A, Yamamoto H, Sato A and Matsumoto S: Wnt5a: its signalling, functions and implication in diseases. Acta Physiol (Oxf). 204:17–33. 2012.

9 

Shen YL, Xu YH, Luo Q, Guo ZH, Zheng GH and Guo YX: Growth and differentiation effect on HL60 cells by adenovirus-mediated exogenous wnt5a gene modification on human bone marrow mesenchymal stem cells. Sichuan Da Xue Xue Bao Yi Xue Ban. 41:931–935. 2010.

10 

Liang H, Chen Q, Coles AH, et al: Wnt5a inhibits B cell proliferation and functions as a tumor suppressor in hematopoietic tissue. Cancer Cell. 4:349–360. 2003.

11 

Shrivastava A, Radziejewski C, Campbell E, et al: An orphan receptor tyrosine kinase family whose members serve as nonintegrin collagen receptors. Mol Cell. 1:25–34. 1997.

12 

Pan Z, Sun X, Shan H, et al: MicroRNA-101 inhibited postinfarct cardiac fibrosis and improved left ventricular compliance via the FBJ osteosarcoma oncogene/transforming growth factor-beta1 pathway. Circulation. 126:840–850. 2012.

13 

Luo J, Deng ZL, Luo X, et al: A protocol for rapid generation of recombinant adenoviruses using the AdEasy system. Nat Protoc. 2:1236–1247. 2007.

14 

van Amerongen R, Fuerer C, Mizutani M and Nusse R: Wnt5a can both activate and repress Wnt/beta-catenin signaling during mouse embryonic development. Dev Biol. 369:101–114. 2012.

15 

Nemeth MJ, Topol L, Anderson SM, Yang Y and Bodine DM: Wnt5a inhibits canonical Wnt signaling in hematopoietic stem cells and enhances repopulation. Proc Natl Acad Sci USA. 104:15436–15441. 2007.

16 

Cheng CW, Yeh JC, Fan TP, Smith SK and Charnock-Jones DS: Wnt5a-mediated non-canonical Wnt signalling regulates human endothelial cell proliferation and migration. Biochem Biophys Res Commun. 365:285–290. 2008.

17 

Koehler A, Schlupf J, Schneider M, Kraft B, Winter C and Kashef J: Loss of Xenopus cadherin-11 leads to increased Wnt/beta-catenin signaling and up-regulation of target genes c-myc and cyclin D1 in neural crest. Dev Biol. 383:132–145. 2013.

18 

Kühl M and Pandur P: Measuring CamKII activity in Xenopus embryos as a read-out for non-canonical Wnt signaling. Methods Mol Biol. 468:173–86. 2008.

19 

Xin H, Xin F, Zhou S and Guan S: The Wnt5a/Ror2 pathway is associated with determination of the differentiation fate of bone marrow mesenchymal stem cells in vascular calcification. Int J Mol Med. 31:583–588. 2013.

20 

Baksh D and Tuan RS: Canonical and non-canonical Wnts differentially affect the development potential of primary isolate of human bone marrow mesenchymal stem cells. J Cell Physiol. 212:817–826. 2007.

21 

de Barros AP, Takiya CM, Garzoni LR, et al: Osteoblasts and bone marrow mesenchymal stromal cells control hematopoietic stem cell migration and proliferation in 3D in vitro model. PLoS One. 5:e90932010.

22 

Takada R, Hijikata H, Kondoh H and Takada S: Analysis of combinatorial effects of Wnts and Frizzleds on beta-catenin/armadillo stabilization and Dishevelled phosphorylation. Genes Cells. 10:919–928. 2005.

23 

Cong F, Schweizer L and Varmus H: Wnt signals across the plasma membrane to activate the beta-catenin pathway by forming oligomers containing its receptors, frizzled and LRP. Development. 131:5103–5115. 2004.

24 

Yamamoto H, Sakane H, Yamamoto H, Michiue T and Kikuchi A: Wnt3a and Dkk1 regulate distinct internalization pathways of LRP6 to tune the activation of beta-catenin signaling. Dev Cell. 15:37–48. 2008.

25 

Torres MA, Yang-Snyder JA, Purcell SM, DeMarais AA, McGrew LL and Moon RT: Activities of the Wnt-1 class of secreted signaling factors are antagonized by the Wnt-5A class and by a dominant negative cadherin in early Xenopus development. J Cell Biol. 133:1123–1137. 1996.

26 

Umbhauer M, Djiane A, Goisset C, et al: The C-terminal cytoplasmic Lys-thr-X-X-X-Trp motif in frizzled receptors mediates Wnt/beta-catenin signalling. EMBO J. 19:4944–4954. 2000.

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Copy and paste a formatted citation
Spandidos Publications style
Shen YL, Luo Q, Guo YX, Zheng GH, Yu J and Xu YH: Bone marrow mesenchymal stem cell‑derived Wnt5a inhibits leukemia cell progression in vitro via activation of the non‑canonical Wnt signaling pathway. Oncol Lett 8: 85-90, 2014.
APA
Shen, Y.L., Luo, Q., Guo, Y.X., Zheng, G.H., Yu, J., & Xu, Y.H. (2014). Bone marrow mesenchymal stem cell‑derived Wnt5a inhibits leukemia cell progression in vitro via activation of the non‑canonical Wnt signaling pathway. Oncology Letters, 8, 85-90. https://doi.org/10.3892/ol.2014.2117
MLA
Shen, Y. L., Luo, Q., Guo, Y. X., Zheng, G. H., Yu, J., Xu, Y. H."Bone marrow mesenchymal stem cell‑derived Wnt5a inhibits leukemia cell progression in vitro via activation of the non‑canonical Wnt signaling pathway". Oncology Letters 8.1 (2014): 85-90.
Chicago
Shen, Y. L., Luo, Q., Guo, Y. X., Zheng, G. H., Yu, J., Xu, Y. H."Bone marrow mesenchymal stem cell‑derived Wnt5a inhibits leukemia cell progression in vitro via activation of the non‑canonical Wnt signaling pathway". Oncology Letters 8, no. 1 (2014): 85-90. https://doi.org/10.3892/ol.2014.2117
Copy and paste a formatted citation
x
Spandidos Publications style
Shen YL, Luo Q, Guo YX, Zheng GH, Yu J and Xu YH: Bone marrow mesenchymal stem cell‑derived Wnt5a inhibits leukemia cell progression in vitro via activation of the non‑canonical Wnt signaling pathway. Oncol Lett 8: 85-90, 2014.
APA
Shen, Y.L., Luo, Q., Guo, Y.X., Zheng, G.H., Yu, J., & Xu, Y.H. (2014). Bone marrow mesenchymal stem cell‑derived Wnt5a inhibits leukemia cell progression in vitro via activation of the non‑canonical Wnt signaling pathway. Oncology Letters, 8, 85-90. https://doi.org/10.3892/ol.2014.2117
MLA
Shen, Y. L., Luo, Q., Guo, Y. X., Zheng, G. H., Yu, J., Xu, Y. H."Bone marrow mesenchymal stem cell‑derived Wnt5a inhibits leukemia cell progression in vitro via activation of the non‑canonical Wnt signaling pathway". Oncology Letters 8.1 (2014): 85-90.
Chicago
Shen, Y. L., Luo, Q., Guo, Y. X., Zheng, G. H., Yu, J., Xu, Y. H."Bone marrow mesenchymal stem cell‑derived Wnt5a inhibits leukemia cell progression in vitro via activation of the non‑canonical Wnt signaling pathway". Oncology Letters 8, no. 1 (2014): 85-90. https://doi.org/10.3892/ol.2014.2117
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