Neurokinin-2 receptor polymorphism predicts lymph node metastasis in colorectal cancer patients

Fang,Weijia; Fu,Caiyun; Chen,Xiaogang; Mou,Xiaozhou; Liu,Fanlong; Qian,Jiong; Zhao,Peng; Zheng,Yulong; Zheng,Yi; Deng,Jing; Ye,Pingjiang; Wang,Yifei; Zheng,Shusen

doi:10.3892/ol.2015.3016

Neurokinin-2 receptor polymorphism predicts lymph node metastasis in colorectal cancer patients

Authors:
- Weijia Fang
- Caiyun Fu
- Xiaogang Chen
- Xiaozhou Mou
- Fanlong Liu
- Jiong Qian
- Peng Zhao
- Yulong Zheng
- Yi Zheng
- Jing Deng
- Pingjiang Ye
- Yifei Wang
- Shusen Zheng
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Affiliations: Biotherapy Center, Department of Medical Oncology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China, Lab of Proteomics and Molecular Enzymology, School of Life Sciences, Zhejiang Sci‑Tech University, Hangzhou, Zhejiang 310018, P.R. China, Department of Anorectum, People's Hospital of Shaoxing, Shaoxing, Zhejiang 312000, P.R. China, Second People's Hospital of Jiaxing, Jiaxing, Zhejiang 314000, P.R. China
Published online on: March 6, 2015 https://doi.org/10.3892/ol.2015.3016
Pages: 2003-2006
Copyright: © Fang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

To analyze the single nucleotide polymorphisms (SNPs) of two subtypes of neurokinin (NK) receptors, NK1R and NK2R (also known as TAC1R and TAC2R), in colorectal cancer (CRC), peripheral blood samples were collected from 199 CRC patients. Direct‑sequencing was performed to identify the NK1R rs10198644 and NK2R rs4644560 SNPs. Genotype results were correlated with clinical factors. The allele frequencies of NK1R rs10198644 GC, CC and GG were 52, 17 and 31%, respectively, while that of NK2R rs4644560 GC, CC, and GG were 36, 50 and 14%, respectively. Patients with NK2R rs4644560 GC exhibited more positive lymph nodes than those with CC (mean, 2.2 vs. 1.3; P=0.016). Further analysis highlighted that the number of positive lymph nodes was also increased in the NK2R rs4644560 GC/NK1R rs10198644 GG group compared with the NK2R rs4644560 GG/NK1R rs10198644 GG group (mean, 2.2 vs. 0.9; P=0.04). These data suggested that the NK2R rs4644560 GC polymorphism alone or combination with NK1R rs10198644 GG may be a promising prognostic marker of lymph node metastasis in CRC patients.

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