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Article

Relative quantitative expression of hypoxia-inducible factor-1α, -2α and -3α, and vascular endothelial growth factor A in laryngeal carcinoma

  • Authors:
    • Todor Miroslavov Popov
    • Teodora Goranova
    • Gergana Stancheva
    • Radka Kaneva
    • Tihomir Dikov
    • Ivan Chalakov
    • Julian Rangachev
    • Dimitar Konov
    • Spiridon Todorov
    • Orlin Stoyanov
    • Vanio Mitev
  • View Affiliations / Copyright

    Affiliations: Department of Ear, Nose and Throat, Medical University of Sofia, Sofia 1680, Bulgaria, Department of Medical Chemistry and Biochemistry and Molecular Medicine Center, Medical University of Sofia, Sofia 1680, Bulgaria, Department of Pathology, Medical University of Sofia, Sofia 1680, Bulgaria
  • Pages: 2879-2885
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    Published online on: March 24, 2015
       https://doi.org/10.3892/ol.2015.3070
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Abstract

The aim of the present study was to determine the relative quantitative expression of hypoxia-inducible factor (HIF)-1α, -2α and -3α, and VEGF-A in laryngeal carcinoma. A total of 63 patients with carcinoma of the larynx were enrolled in the study. Total RNA was isolated from fresh, frozen normal and tumor tissues of each patient, and quantitative polymerase chain reaction was performed. HIF‑1α was upregulated in the majority of patients (44 patients; 69.84%). By contrast, only 7 (11.11%) patients from the whole group displayed HIF‑2α overexpression, while the HIF‑3α isoform was silenced in the majority of patients (48 patients, 76.19%). A small group of 5 (7.94%) patients exhibited significant overexpression of the HIF‑3α isoform. VEGF‑A expression was significantly higher (P<0.05) in patients with upregulated HIF‑1α (2.72±1.41 RQ) compared with patients without upregulated HIF‑1α (1.86±1.46 RQ). There was a moderate positive correlation between mRNA expression levels of HIF‑1α and VEGF‑A (rs=0.392; P<0.005). To the best of our knowledge, this study is first to report quantitative data with regard to the expression of all three HIF isoforms in malignant neoplasms. The findings suggest the existence of specific phenotypes of HIF expression in laryngeal carcinoma, where the HIF switch is absent.
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1 

Wang GL, Jiang BH, Rue EA and Semenza GL: Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension. Proc Natl Acad Sci USA. 92:5510–5514. 1995. View Article : Google Scholar : PubMed/NCBI

2 

Wiesener MS, Turley H, Allen WE, et al: Induction of endothelial PAS domain protein-1 by hypoxia: Characterization and comparison with hypoxia-inducible factor-1alpha. Blood. 92:2260–2268. 1998.PubMed/NCBI

3 

Heidbreder M, Fröhlich F, Jöhren O, et al: Hypoxia rapidly activates HIF-3alpha mRNA expression. FASEB J. 17:1541–1543. 2003.PubMed/NCBI

4 

Li QF, Wang XR, Yang YW and Lin H: Hypoxia upregulates hypoxia-inducible factor (HIF)-3alpha expression in lung epithelial cells: Characterization and comparison with HIF-1alpha. Cell Res. 16:548–558. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Pugh CW, O'Rourke JF, Nagao M, et al: Activation of hypoxia-inducible factor-1; definition of regulatory domains within the alpha subunit. J Biol Chem. 272:11205–11214. 1997. View Article : Google Scholar : PubMed/NCBI

6 

Ikeda E, Achen MG, Breier G and Risau W: Hypoxia-induced transcriptional activation and increased mRNA stability of vascular endothelial growth factor in C6 glioma cells. J Biol Chem. 270:19761–19766. 1995. View Article : Google Scholar : PubMed/NCBI

7 

Forsythe JA, Jiang BH, Iyer NV, et al: Activation of vascular endothelial growth factor gene transcription by hypoxia-inducible factor 1. Mol Cell Biol. 16:4604–4613. 1996.PubMed/NCBI

8 

Takeda N, Maemura K, Imai Y, et al: Endothelial PAS domain protein 1 gene promotes angiogenesis through the transactivation of both vascular endothelial growth factor and its receptor, Flt-1. Circ Res. 95:146–153. 2004. View Article : Google Scholar : PubMed/NCBI

9 

Gerber HP, Condorelli F, Park J and Ferrara N: Differential transcriptional regulation of the two vascular endothelial growth factor receptor genes. Flt-1, but not Flk-1/KDR, is up-regulated by hypoxia. J Biol Chem. 272:23659–23667. 1997. View Article : Google Scholar : PubMed/NCBI

10 

Marti HH and Risau W: Systemic hypoxia changes the organ-specific distribution of vascular endothelial growth factor and its receptors. Proc Natl Acad Sci USA. 95:15809–15814. 1998. View Article : Google Scholar : PubMed/NCBI

11 

Semenza GL and Wang GL: A nuclear factor induced by hypoxia via de novo protein synthesis binds to the human erythropoietin gene enhancer at a site required for transcriptional activation. Mol Cell Biol. 12:5447–5454. 1992.PubMed/NCBI

12 

Kertesz N, Wu J, Chen TH, et al: The role of erythropoietin in regulating angiogenesis. Dev Biol. 276:101–110. 2004. View Article : Google Scholar : PubMed/NCBI

13 

Jaquet K, Krause K, Tawakol-Khodai M, Geidel S and Kuck KH: Erythropoietin and VEGF exhibit equal angiogenic potential. Microvasc Res. 64:326–333. 2002. View Article : Google Scholar : PubMed/NCBI

14 

Morita M, Ohneda O, Yamashita T, et al: HLF/HIF-2alpha is a key factor in retinopathy of prematurity in association with erythropoietin. EMBO J. 22:1134–1146. 2003. View Article : Google Scholar : PubMed/NCBI

15 

Hirota K and Semenza GL: Regulation of angiogenesis by hypoxia-inducible factor 1. Crit Rev Oncol Hematol. 59:15–26. 2006. View Article : Google Scholar : PubMed/NCBI

16 

Yu J, de Muinck ED, Zhuang Z, et al: Endothelial nitric oxide synthase is critical for ischemic remodeling, mural cell recruitment, and blood flow reserve. Proc Natl Acad Sci USA. 102:10999–11004. 2005. View Article : Google Scholar : PubMed/NCBI

17 

Coulet F, Nadaud S, Agrapart M and Soubrier F: Identification of hypoxia-response element in the human endothelial nitric-oxide synthase gene promoter. J Biol Chem. 278:46230–46240. 2003. View Article : Google Scholar : PubMed/NCBI

18 

Zhao X, Lu X and Feng Q: Deficiency in endothelial nitric oxide synthase impairs myocardial angiogenesis. Am J Physiol Heart Circ Physiol. 283:H2371–H2378. 2002. View Article : Google Scholar : PubMed/NCBI

19 

Maynard MA, Qi H, Chung J, et al: Multiple splice variants of the human HIF-3 alpha locus are targets of the von Hippel-Lindau E3 ubiquitin ligase complex. J Biol Chem. 278:11032–11040. 2003. View Article : Google Scholar : PubMed/NCBI

20 

Strauss L, Volland D, Kunkel M and Reichert TE: Dual role of VEGF family members in the pathogenesis of head and neck cancer (HNSCC): Possible link between angiogenesis and immune tolerance. Med Sci Monit. 11:BR280–BR292. 2005.PubMed/NCBI

21 

Alon T, Hemo I, Itin A, Pe'er J, Stone J and Keshet E: Vascular endothelial growth factor acts as a survival factor for newly formed retinal vessels and has implications for retinopathy of prematurity. Nat Med. 1:1024–1028. 1995. View Article : Google Scholar : PubMed/NCBI

22 

Gerber HP, Dixit V and Ferrara N: Vascular endothelial growth factor induces expression of the antiapoptotic proteins Bcl-2 and A1 in vascular endothelial cells. J Biol Chem. 273:13313–13316. 1998. View Article : Google Scholar : PubMed/NCBI

23 

Gerber HP, McMurtrey A, Kowalski J, et al: Vascular endothelial growth factor regulates endothelial cell survival through the phosphatidylinositol 3′-kinase/Akt signal transduction pathway. Requirement for Flk-1/KDR activation. J Biol Chem. 273:30336–30343. 1998. View Article : Google Scholar : PubMed/NCBI

24 

Benjamin LE, Golijanin D, Itin A, Pode D and Keshet E: Selective ablation of immature blood vessels in established human tumors follows vascular endothelial growth factor withdrawal. J Clin Invest. 103:159–165. 1999. View Article : Google Scholar : PubMed/NCBI

25 

Yuan F, Chen Y, Dellian M, Safabakhsh N, Ferrara N and Jain RK: Time-dependent vascular regression and permeability changes in established human tumor xenografts induced by an anti-vascular endothelial growth factor/vascular permeability factor antibody. Proc Natl Acad Sci USA. 93:14765–14770. 1996. View Article : Google Scholar : PubMed/NCBI

26 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

27 

Scrideli CA, Carlotti CG Jr, Okamoto OK, et al: Gene expression profile analysis of primary glioblastomas and non-neoplastic brain tissue: Identification of potential target genes by oligonucleotide microarray and real-time quantitative PCR. J Neurooncol. 88:281–291. 2008. View Article : Google Scholar : PubMed/NCBI

28 

Borel F, Han R, Visser A, Petry H, van Deventer SJ, Jansen PL and Konstantinova P: Réseau Centre de Ressources Biologiques Foie (French Liver Biobanks Network), France: Adenosine triphosphate-binding cassette transporter genes up-regulation in untreated hepatocellular carcinoma is mediated by cellular microRNAs. Hepatology. 55:821–832. 2012. View Article : Google Scholar : PubMed/NCBI

29 

International Union Against Cancer: Head and Neck Tumours. In: TNM Classification of Malignant Tumours (7th). Sobin LH, Gospodarowicz MK and Wittekind C: Wiley-Blackwell. 39–46. 2009.

30 

Moreno-Galindo C, Hermsen M, Garcia-Pedrero JM, Fresno MF, Suarez C and Rodrigo JP: p27 and BCL2 expression predicts response to chemotherapy in head and neck squamous cell carcinomas. Oral Oncol. 50:128–134. 2014. View Article : Google Scholar : PubMed/NCBI

31 

Wachters JE, Schrijvers ML, Slagter-Menkema L, Mastik M, de Bock GH, Langendijk JA, et al: Prognostic significance of HIF-1a, CA-IX, and OPN in T1-T2 laryngeal carcinoma treated with radiotherapy. Laryngoscope. 123:2154–2160. 2013. View Article : Google Scholar : PubMed/NCBI

32 

Li DW, Dong P, Wang F, Chen XW, Xu CZ and Zhou L: Hypoxia induced multidrug resistance of laryngeal cancer cells via hypoxia-inducible factor-1α. Asian Pac J Cancer Prev. 14:4853–4858. 2013. View Article : Google Scholar : PubMed/NCBI

33 

Xie J, Li DW, Chen XW, Wang F and Dong P: Expression and significance of hypoxia-inducible factor-1α and MDR1/P-glycoprotein in laryngeal carcinoma tissue and hypoxic Hep-2 cells. Oncol Lett. 6:232–238. 2013.PubMed/NCBI

34 

Wu XH, Chen SP, Mao JY, Ji XX, Yao HT and Zhou SH: Expression and significance of hypoxia-inducible factor-1α and glucose transporter-1 in laryngeal carcinoma. Oncol Lett. 5:261–266. 2013.PubMed/NCBI

35 

Li DW, Zhou L, Jin B, Xie J and Dong P: Expression and significance of hypoxia-inducible factor-1α and survivin in laryngeal carcinoma tissue and cells. Otolaryngol Head Neck Surg. 148:75–81. 2013. View Article : Google Scholar : PubMed/NCBI

36 

Douglas CM, Bernstein JM, Ormston VE, et al: Lack of prognostic effect of carbonic anhydrase-9, hypoxia inducible factor-1α and bcl-2 in 286 patients with early squamous cell carcinoma of the glottic larynx treated with radiotherapy. Clin Oncol (R Coll Radiol). 25:59–65. 2013. View Article : Google Scholar : PubMed/NCBI

37 

Wu XH, Lu YF, Hu XD, Mao JY, Ji XX, Yao HT, et al: Expression of hypoxia inducible factor-1α and its significance in laryngeal carcinoma. J Int Med Res. 38:2040–2046. 2010. View Article : Google Scholar : PubMed/NCBI

38 

Moon SY, Chang HW, Roh JL, et al: Using YC-1 to overcome the radioresistance of hypoxic cancer cells. Oral Oncol. 45:915–919. 2009. View Article : Google Scholar : PubMed/NCBI

39 

Cabanillas R, Rodrigo JP, Secades P, et al: The relation between hypoxia-inducible factor (HIF)-1alpha expression with p53 expression and outcome in surgically treated supraglottic laryngeal cancer. J Surg Oncol. 99:373–378. 2009. View Article : Google Scholar : PubMed/NCBI

40 

Wildeman MA, Gibcus JH, Hauptmann M, et al: Radiotherapy in laryngeal carcinoma: can a panel of 13 markers predict response? Laryngoscope. 119:316–322. 2009. View Article : Google Scholar : PubMed/NCBI

41 

Kyzas PA, Stefanou D, Batistatou A and Agnantis NJ: Hypoxia-induced tumor angiogenic pathway in head and neck cancer: an in vivo study. Cancer Lett. 225:297–304. 2005. View Article : Google Scholar : PubMed/NCBI

42 

Yu L, Liu Y and Cui Y: Expression of hypoxia inducible factor-1alpha and its relationship to apoptosis and proliferation in human laryngeal squamous cell carcinoma. J Huazhong Univ Sci Technolog Med Sci. 24:636–638. 2004. View Article : Google Scholar : PubMed/NCBI

43 

Keith B, Johnson RS and Simon MC: HIF1α and HIF2α: Sibling rivalry in hypoxic tumour growth and progression. Nat Rev Cancer. 12:9–22. 2012.

44 

Holmquist-Mengelbier L, Fredlund E, Löfstedt T, et al: Recruitment of HIF-1alpha and HIF-2alpha to common target genes is differentially regulated in neuroblastoma: HIF-2alpha promotes an aggressive phenotype. Cancer Cell. 10:413–423. 2006. View Article : Google Scholar : PubMed/NCBI

45 

Koh MY, Lemos R Jr, Liu X and Powis G: The hypoxia-associated factor switches cells from HIF-1α- to HIF-2α-dependent signaling promoting stem cell characteristics, aggressive tumor growth and invasion. Cancer Res. 71:4015–4027. 2011. View Article : Google Scholar : PubMed/NCBI

46 

Koh MY and Powis G: Passing the baton: The HIF switch. Trends Biochem Sci. 37:364–372. 2012. View Article : Google Scholar : PubMed/NCBI

47 

Raval RR, Lau KW, Tran MG, Sowter HM, Mandriota SJ, Li JL, Pugh CW, Maxwell PH, Harris AL and Ratcliffe PJ: Contrasting properties of hypoxia-inducible factor 1 (HIF-1) and HIF-2 in von Hippel-Lindau-associated renal cell carcinoma. Mol Cell Biol. 25:5675–5686. 2005. View Article : Google Scholar : PubMed/NCBI

48 

Mandriota SJ, Turner KJ, Davies DR, et al: HIF activation identifies early lesions in VHL kidneys: Evidence for site-specific tumor suppressor function in the nephron. Cancer Cell. 1:459–468. 2002. View Article : Google Scholar : PubMed/NCBI

49 

Fong GH: Mechanisms of adaptive angiogenesis to tissue hypoxia. Angiogenesis. 11:121–140. 2008. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Popov TM, Goranova T, Stancheva G, Kaneva R, Dikov T, Chalakov I, Rangachev J, Konov D, Todorov S, Stoyanov O, Stoyanov O, et al: Relative quantitative expression of hypoxia-inducible factor-1α, -2α and -3α, and vascular endothelial growth factor A in laryngeal carcinoma. Oncol Lett 9: 2879-2885, 2015.
APA
Popov, T.M., Goranova, T., Stancheva, G., Kaneva, R., Dikov, T., Chalakov, I. ... Mitev, V. (2015). Relative quantitative expression of hypoxia-inducible factor-1α, -2α and -3α, and vascular endothelial growth factor A in laryngeal carcinoma. Oncology Letters, 9, 2879-2885. https://doi.org/10.3892/ol.2015.3070
MLA
Popov, T. M., Goranova, T., Stancheva, G., Kaneva, R., Dikov, T., Chalakov, I., Rangachev, J., Konov, D., Todorov, S., Stoyanov, O., Mitev, V."Relative quantitative expression of hypoxia-inducible factor-1α, -2α and -3α, and vascular endothelial growth factor A in laryngeal carcinoma". Oncology Letters 9.6 (2015): 2879-2885.
Chicago
Popov, T. M., Goranova, T., Stancheva, G., Kaneva, R., Dikov, T., Chalakov, I., Rangachev, J., Konov, D., Todorov, S., Stoyanov, O., Mitev, V."Relative quantitative expression of hypoxia-inducible factor-1α, -2α and -3α, and vascular endothelial growth factor A in laryngeal carcinoma". Oncology Letters 9, no. 6 (2015): 2879-2885. https://doi.org/10.3892/ol.2015.3070
Copy and paste a formatted citation
x
Spandidos Publications style
Popov TM, Goranova T, Stancheva G, Kaneva R, Dikov T, Chalakov I, Rangachev J, Konov D, Todorov S, Stoyanov O, Stoyanov O, et al: Relative quantitative expression of hypoxia-inducible factor-1α, -2α and -3α, and vascular endothelial growth factor A in laryngeal carcinoma. Oncol Lett 9: 2879-2885, 2015.
APA
Popov, T.M., Goranova, T., Stancheva, G., Kaneva, R., Dikov, T., Chalakov, I. ... Mitev, V. (2015). Relative quantitative expression of hypoxia-inducible factor-1α, -2α and -3α, and vascular endothelial growth factor A in laryngeal carcinoma. Oncology Letters, 9, 2879-2885. https://doi.org/10.3892/ol.2015.3070
MLA
Popov, T. M., Goranova, T., Stancheva, G., Kaneva, R., Dikov, T., Chalakov, I., Rangachev, J., Konov, D., Todorov, S., Stoyanov, O., Mitev, V."Relative quantitative expression of hypoxia-inducible factor-1α, -2α and -3α, and vascular endothelial growth factor A in laryngeal carcinoma". Oncology Letters 9.6 (2015): 2879-2885.
Chicago
Popov, T. M., Goranova, T., Stancheva, G., Kaneva, R., Dikov, T., Chalakov, I., Rangachev, J., Konov, D., Todorov, S., Stoyanov, O., Mitev, V."Relative quantitative expression of hypoxia-inducible factor-1α, -2α and -3α, and vascular endothelial growth factor A in laryngeal carcinoma". Oncology Letters 9, no. 6 (2015): 2879-2885. https://doi.org/10.3892/ol.2015.3070
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