Evodiamine inhibits proliferation and induces apoptosis in gastric cancer cells

Shen,Huamei; Zhao,Shuai; Xu,Zhipeng; Zhu,Lihua; Han,Yuehua; Ye,Jun

doi:10.3892/ol.2015.3153

July-2015 Volume 10 Issue 1

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July-2015 Volume 10 Issue 1

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Article

Evodiamine inhibits proliferation and induces apoptosis in gastric cancer cells

Authors:
- Huamei Shen
- Shuai Zhao
- Zhipeng Xu
- Lihua Zhu
- Yuehua Han
- Jun Ye
View Affiliations / Copyright

Affiliations: Department of Infectious Disease, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, P.R. China, Department of Gastroenterology, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, P.R. China, Department of Hyperbaric Oxygen Chamber, The Xinhua Hospital of Zhejiang, Hangzhou, Zhejiang 310009, P.R. China
Pages: 367-371
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Published online on: April 27, 2015

https://doi.org/10.3892/ol.2015.3153
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Abstract

In the present study, the effects of evodiamine on the apoptosis of human gastric cancer cells was studied in order to assess its antitumor effects and identify the molecular mechanisms involved. SGC7901 gastric cancer cells were treated with evodiamine at various concentrations (0, 3, 6, 12, 24 and 48 µmol/l) for 24 h. Inhibition of the proliferation of SGC7901 cells was assessed using an MTT assay. The morphology of treated SGC7901 cells was observed using optical microscopy; in addition, the effect of evodiamine on the nuclear morphology of cells was analyzed using Hoechst 33258 staining with fluorescence microscopy. Annexin V‑fluorescein isothiocyanate/propidium iodide staining and flow cytometric analysis were used for investigating the effect of evodiamine on the induction of apoptosis in SGC7901 cells. Expression levels of survivin and caspase‑3 were examined using reverse transcription polymerase chain reaction. The results demonstrated that evodiamine significantly inhibited SGC7901 cell proliferation (P<0.05) and induced apoptosis in a dose‑dependent manner (P<0.05). Morphological characteristics of apoptosis were confirmed using optical microscopy and Hoechst 33258 staining analysis indicated that evodiamine treatment resulted in the typical characteristics of apoptotic programmed cell death, including cell shrinkage and apoptotic body formation. Flow cytometric analysis demonstrated that evodiamine induced the dose‑dependent apoptosis of SGC7901 cells. Messenger (m)RNA levels of survivin decreased and those of caspase‑3 increase in a dose‑dependent manner in SGC7901 cells treated with various concentrations of evodiamine for 24 h. In conclusion, the results of the present study demonstrated that evodiamine inhibited proliferation and induced apoptosis in gastric cancer cells via the downregulation of survivin and upregulation of caspase-3 mRNA.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Evodiamine inhibits proliferation and induces apoptosis in gastric cancer cells

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