Radiosensitization of human glioma cells by tamoxifen is associated with the inhibition of PKC-ι activity in vitro

  • Authors:
    • Lei Yang
    • Xiaopeng Yuan
    • Jie Wang
    • Cheng Gu
    • Haowen Zhang
    • Jiahua Yu
    • Fenju Liu
  • View Affiliations

  • Published online on: May 11, 2015     https://doi.org/10.3892/ol.2015.3195
  • Pages: 473-478
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The present study aimed to investigate the radiosensitizing effects of tamoxifen (TAM), a non-steroidal anti-estrogen drug, in human glioma A172 and U251 cells in vitro. A colony‑forming assay revealed that TAM enhances radiosensitivity in A172 and U251 cells. Treatment with TAM also increased the percentage of apoptotic cells subsequent to ionizing radiation, and increased the expression of apoptotic markers, including cleaved caspase‑3 and poly(ADP‑ribose) polymerase. Ionizing radiation induced G2/M phase arrest, which was alleviated within 24 h when the radiation‑induced DNA damage was repaired. However, flow cytometry analysis revealed that TAM treatment delayed the recovery of cell cycle progression. Additional examination demonstrated that TAM‑mediated protein kinase C‑ι (PKC‑ι) inhibition may lead to the activation of pro‑apoptotic B‑cell lymphoma 2‑associated death promoter, and the dephosphorylation of cyclin‑dependent kinase 7, resulting in increased cell apoptosis and sustained G2/M phase arrest following exposure to radiation. The present data indicate that the radiosensitizing effects of TAM on glioma cells are partly due to the inhibition of PKC-ι activity in vitro.
View Figures
View References

Related Articles

Journal Cover

July-2015
Volume 10 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Yang L, Yuan X, Wang J, Gu C, Zhang H, Yu J and Liu F: Radiosensitization of human glioma cells by tamoxifen is associated with the inhibition of PKC-ι activity in vitro. Oncol Lett 10: 473-478, 2015
APA
Yang, L., Yuan, X., Wang, J., Gu, C., Zhang, H., Yu, J., & Liu, F. (2015). Radiosensitization of human glioma cells by tamoxifen is associated with the inhibition of PKC-ι activity in vitro. Oncology Letters, 10, 473-478. https://doi.org/10.3892/ol.2015.3195
MLA
Yang, L., Yuan, X., Wang, J., Gu, C., Zhang, H., Yu, J., Liu, F."Radiosensitization of human glioma cells by tamoxifen is associated with the inhibition of PKC-ι activity in vitro". Oncology Letters 10.1 (2015): 473-478.
Chicago
Yang, L., Yuan, X., Wang, J., Gu, C., Zhang, H., Yu, J., Liu, F."Radiosensitization of human glioma cells by tamoxifen is associated with the inhibition of PKC-ι activity in vitro". Oncology Letters 10, no. 1 (2015): 473-478. https://doi.org/10.3892/ol.2015.3195