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Article Open Access

In vitro and in vivo antitumor effects of 50 to 100‑KDa components from B16 melanoma culture supernatant

  • Authors:
    • Ying‑Song Qin
    • Xu Zhang
    • Xiang‑Yu Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Pathophysiology, Xuzhou Medical College, Xuzhou, Jiangsu, P.R. China, Department of Otolaryngological, Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu, P.R. China, State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, P.R. China
    Copyright: © Qin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 149-155
    |
    Published online on: May 20, 2015
       https://doi.org/10.3892/ol.2015.3239
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Abstract

The development of immunological therapies for melanoma has been of considerable concern in recent years. Whole tumor cell lysates have been used to develop antitumor vaccines, but the effective components of the lysates have not been identified. In the present study, protein elements were purified from the B16 supernatant to analyze the in vitro chemotaxis towards mouse spleen lymphocytes using a Boyden chamber. Prior to establishing a B16 melanoma model, C57BL/6 mice were vaccinated with these proteins, and melanoma growth, tumor appearance time and behavioral changes were observed. Next, the cytotoxicity and subsets of the tumor infiltrating lymphocytes, and the histological characteristics of the melanoma were analyzed. The isolated purified fragments of B16 melanoma culture supernatant had strong antitumor effects. The possible antitumor mechanism was delineated, and was identified to possibly be through the activation of cluster of differentiation 8‑positive T cells and the promotion of B16 cell differentiation. These methods will provide a novel insight into understanding antitumor immunological mechanisms and provide a potential avenue for immunotherapy.
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Copy and paste a formatted citation
Spandidos Publications style
Qin YS, Zhang X and Zhang XY: In vitro and in vivo antitumor effects of 50 to 100‑KDa components from B16 melanoma culture supernatant. Oncol Lett 10: 149-155, 2015.
APA
Qin, Y., Zhang, X., & Zhang, X. (2015). In vitro and in vivo antitumor effects of 50 to 100‑KDa components from B16 melanoma culture supernatant. Oncology Letters, 10, 149-155. https://doi.org/10.3892/ol.2015.3239
MLA
Qin, Y., Zhang, X., Zhang, X."In vitro and in vivo antitumor effects of 50 to 100‑KDa components from B16 melanoma culture supernatant". Oncology Letters 10.1 (2015): 149-155.
Chicago
Qin, Y., Zhang, X., Zhang, X."In vitro and in vivo antitumor effects of 50 to 100‑KDa components from B16 melanoma culture supernatant". Oncology Letters 10, no. 1 (2015): 149-155. https://doi.org/10.3892/ol.2015.3239
Copy and paste a formatted citation
x
Spandidos Publications style
Qin YS, Zhang X and Zhang XY: In vitro and in vivo antitumor effects of 50 to 100‑KDa components from B16 melanoma culture supernatant. Oncol Lett 10: 149-155, 2015.
APA
Qin, Y., Zhang, X., & Zhang, X. (2015). In vitro and in vivo antitumor effects of 50 to 100‑KDa components from B16 melanoma culture supernatant. Oncology Letters, 10, 149-155. https://doi.org/10.3892/ol.2015.3239
MLA
Qin, Y., Zhang, X., Zhang, X."In vitro and in vivo antitumor effects of 50 to 100‑KDa components from B16 melanoma culture supernatant". Oncology Letters 10.1 (2015): 149-155.
Chicago
Qin, Y., Zhang, X., Zhang, X."In vitro and in vivo antitumor effects of 50 to 100‑KDa components from B16 melanoma culture supernatant". Oncology Letters 10, no. 1 (2015): 149-155. https://doi.org/10.3892/ol.2015.3239
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