Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
August-2015 Volume 10 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2015 Volume 10 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Effect of vascular endothelial growth factor‑C expression on lymph node metastasis in human cholangiocarcinoma

  • Authors:
    • Rui Zhao
    • Yuan Chang
    • Zhao Liu
    • Yanfeng Liu
    • Sen Guo
    • Jian Yu
    • Jiayong Wang
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery, Qilu Hospital, Shandong University, Jinan, Shandong 250012, P.R. China, Department of Anesthesiology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China, Department of Hepatobiliary and Pancreatic Surgery, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong 250013, P.R. China
  • Pages: 1011-1015
    |
    Published online on: June 2, 2015
       https://doi.org/10.3892/ol.2015.3309
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The present study aimed to evaluate the expression of vascular endothelial growth factor C (VEGF‑C) in human cholangiocarcinoma tissues and its role in metastasis in vitro. A total of 65 biopsy samples of cholangiocarcinoma, plus the FRH‑0201 cell line, were investigated. The expression of VEGF‑C in the human cholangiocarcinoma specimens was evaluated by immunohistochemistry (IHC). The effect of VEGF‑C on tumor cell migration and proliferation was measured by MTT and Transwell assays in the FRH‑0201 cell line. According to the IHC results, the biopsies of human cholangiocarcinoma were stained positively for VEGF‑C, with a positive rate of 75.4% (49/65). Moreover, VEGF‑C was expressed at a higher level in the patients with lymph node metastasis than in those without lymph node metastasis. In vitro, VEGF‑C exhibited marked growth stimulation below the concentration of 5 ng/ml and was able to promote cholangiocarcinoma cell migration significantly. These findings suggested that VEGF‑C may be a useful factor to predict lymph node metastasis in cholangiocarcinoma tissues and indicates that VEGF‑C plays a significant role in proliferation and migration in cholangiocarcinoma cells.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Patel T: Increasing incidence and mortality of primary intrahepatic cholangiocarcinoma in the United States. Hepatology. 33:1353–1357. 2001. View Article : Google Scholar : PubMed/NCBI

2 

Gores GJ: Cholangiocarcinoma: Current concepts and insights. Hepatology. 37:961–969. 2003. View Article : Google Scholar : PubMed/NCBI

3 

Wu L, Li X, Ye L, et al: Vascular endothelial growth inhibitor is a negative regulator of aggressiveness and microvascular density in human clear cell renal cell carcinoma. Anticancer Res. 34:715–722. 2014.PubMed/NCBI

4 

Lozano-Santos C, Martinez-Velasquez J, Fernandez-Cuevas B, et al: Vascular endothelial growth factor A (VEGFA) gene polymorphisms have an impact on survival in a subgroup of indolent patients with chronic lymphocytic leukemia. PLoS One. 27:e1010632014. View Article : Google Scholar

5 

Zhao R, Liu XQ, Wu XP, et al: Vascular endothelial growth factor (VEGF) enhances gastric carcinoma invasiveness via integrin alpha(v)beta6. Cancer Lett. 287:150–156. 2010. View Article : Google Scholar : PubMed/NCBI

6 

Skobe M, Hamberg LM, Hawighorst T, et al: Concurrent induction of lymphangiogenesis, angiogenesis and macrophage recruitment by vascular endothelial growth factor-C in melanoma. Am J Pathol. 159:893–903. 2001. View Article : Google Scholar : PubMed/NCBI

7 

Decio A, Taraboletti G, Patton V, et al: Vascular endothelial growth factor c promotes ovarian carcinoma progression through paracrine and autocrine mechanisms. Am J Pathol. 184:1050–1061. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Liu J, Liu C, Qiu L, Li J, Zhang P and Sun Y: Overexpression of both platelet-derived growth factor-BB and vascular endothelial growth factor-C and its association with lymphangiogenesis in primary human non-small cell lung cancer. Diagn Pathol. 9:1282014. View Article : Google Scholar : PubMed/NCBI

9 

Ciobanu M, Eremia IA, Crăiţoiu S, et al: Lymphatic microvessels density, VEGF-C and VEGFR-3 expression in 25 cases of breast invasive lobular carcinoma. Rom J Morphol Embryol. 54:925–934. 2013.PubMed/NCBI

10 

Ishikawa M, Kitayama J, Kazama S and Nagawa H: Expression of vascular endothelial growth factor C and D (VEGF-C and -D) is an important risk factor for lymphatic metastasis in undifferentiated early gastric carcinoma. Jpn J Clin Oncol. 33:21–27. 2003. View Article : Google Scholar : PubMed/NCBI

11 

Yonemura Y, Endo Y, Fujita H, et al: Role of vascular endothelial growth factor C expression in the development of lymph node metastasis in gastric cancer. Clin Cancer Res. 5:1823–1829. 1999.PubMed/NCBI

12 

Kono M, Watanabe M, Abukawa H, Hasegawa O, Satomi T and Chikazu D: Cyclo-oxygenase-2 expression is associated with vascular endothelial growth factor C expression and lymph node metastasis in oral squamous cell carcinoma. J Oral Maxillofac Surg. 71:1694–1702. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Rubbia-Brandt L, Terris B, Giostra E, Dousset B, Morel P and Pepper MS: Lymphatic vessel density and vascular endothelial growth factor-C expression correlate with malignant behavior in human pancreatic endocrine tumors. Clin Cancer Res. 10:6919–6928. 2004. View Article : Google Scholar : PubMed/NCBI

14 

Aishima S, Nishihara Y, Iguchi T, Taguchi K, Taketomi A, Maehara Y and Tsuneyoshi M: Lymphatic spread is related to VEGF-C expression and D2-40-positive myofibroblasts in intrahepatic cholangiocarcinoma. Mod Pathol. 21:256–264. 2008. View Article : Google Scholar : PubMed/NCBI

15 

Wang WB, Li YH, Liu B, Wang HS, Cui AR and Zhnag XH: Correlation between PPARgamma and VEGF-C expression in extrahepatic cholangioadenocarcinoma (EHCAC) and their prognostic significance. Zhonghua Zhong Liu Za Zhi. 31:773–777. 2009.(In Chinese). PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhao R, Chang Y, Liu Z, Liu Y, Guo S, Yu J and Wang J: Effect of vascular endothelial growth factor‑C expression on lymph node metastasis in human cholangiocarcinoma. Oncol Lett 10: 1011-1015, 2015.
APA
Zhao, R., Chang, Y., Liu, Z., Liu, Y., Guo, S., Yu, J., & Wang, J. (2015). Effect of vascular endothelial growth factor‑C expression on lymph node metastasis in human cholangiocarcinoma. Oncology Letters, 10, 1011-1015. https://doi.org/10.3892/ol.2015.3309
MLA
Zhao, R., Chang, Y., Liu, Z., Liu, Y., Guo, S., Yu, J., Wang, J."Effect of vascular endothelial growth factor‑C expression on lymph node metastasis in human cholangiocarcinoma". Oncology Letters 10.2 (2015): 1011-1015.
Chicago
Zhao, R., Chang, Y., Liu, Z., Liu, Y., Guo, S., Yu, J., Wang, J."Effect of vascular endothelial growth factor‑C expression on lymph node metastasis in human cholangiocarcinoma". Oncology Letters 10, no. 2 (2015): 1011-1015. https://doi.org/10.3892/ol.2015.3309
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao R, Chang Y, Liu Z, Liu Y, Guo S, Yu J and Wang J: Effect of vascular endothelial growth factor‑C expression on lymph node metastasis in human cholangiocarcinoma. Oncol Lett 10: 1011-1015, 2015.
APA
Zhao, R., Chang, Y., Liu, Z., Liu, Y., Guo, S., Yu, J., & Wang, J. (2015). Effect of vascular endothelial growth factor‑C expression on lymph node metastasis in human cholangiocarcinoma. Oncology Letters, 10, 1011-1015. https://doi.org/10.3892/ol.2015.3309
MLA
Zhao, R., Chang, Y., Liu, Z., Liu, Y., Guo, S., Yu, J., Wang, J."Effect of vascular endothelial growth factor‑C expression on lymph node metastasis in human cholangiocarcinoma". Oncology Letters 10.2 (2015): 1011-1015.
Chicago
Zhao, R., Chang, Y., Liu, Z., Liu, Y., Guo, S., Yu, J., Wang, J."Effect of vascular endothelial growth factor‑C expression on lymph node metastasis in human cholangiocarcinoma". Oncology Letters 10, no. 2 (2015): 1011-1015. https://doi.org/10.3892/ol.2015.3309
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team