Effect of vascular endothelial growth factor‑C expression on lymph node metastasis in human cholangiocarcinoma

Zhao,Rui; Chang,Yuan; Liu,Zhao; Liu,Yanfeng; Guo,Sen; Yu,Jian; Wang,Jiayong

doi:10.3892/ol.2015.3309

Effect of vascular endothelial growth factor‑C expression on lymph node metastasis in human cholangiocarcinoma

Authors:
- Rui Zhao
- Yuan Chang
- Zhao Liu
- Yanfeng Liu
- Sen Guo
- Jian Yu
- Jiayong Wang
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Affiliations: Department of Hepatobiliary Surgery, Qilu Hospital, Shandong University, Jinan, Shandong 250012, P.R. China, Department of Anesthesiology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China, Department of Hepatobiliary and Pancreatic Surgery, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong 250013, P.R. China
Published online on: June 2, 2015 https://doi.org/10.3892/ol.2015.3309
Pages: 1011-1015

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Abstract

The present study aimed to evaluate the expression of vascular endothelial growth factor C (VEGF‑C) in human cholangiocarcinoma tissues and its role in metastasis in vitro. A total of 65 biopsy samples of cholangiocarcinoma, plus the FRH‑0201 cell line, were investigated. The expression of VEGF‑C in the human cholangiocarcinoma specimens was evaluated by immunohistochemistry (IHC). The effect of VEGF‑C on tumor cell migration and proliferation was measured by MTT and Transwell assays in the FRH‑0201 cell line. According to the IHC results, the biopsies of human cholangiocarcinoma were stained positively for VEGF‑C, with a positive rate of 75.4% (49/65). Moreover, VEGF‑C was expressed at a higher level in the patients with lymph node metastasis than in those without lymph node metastasis. In vitro, VEGF‑C exhibited marked growth stimulation below the concentration of 5 ng/ml and was able to promote cholangiocarcinoma cell migration significantly. These findings suggested that VEGF‑C may be a useful factor to predict lymph node metastasis in cholangiocarcinoma tissues and indicates that VEGF‑C plays a significant role in proliferation and migration in cholangiocarcinoma cells.

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