Metastasis of myxoid liposarcoma to fat‑bearing areas: A case report of unusual metastatic sites and a hypothesis
- Authors:
- Published online on: August 10, 2015 https://doi.org/10.3892/ol.2015.3585
- Pages: 2543-2546
Abstract
Introduction
Liposarcoma (LPS) contains three subtypes: i) Well-differentiated LPS and dedifferentiated LPS; ii) myxoid LPS (MLPS); and iii) pleomorphic LPS. MLPS accounts for ~30% of all LPS cases and is characterized by the appearance of uniform, round- to oval-shaped cells with myxoid stroma (1,2). For local disease, the 5-year disease-specific survival (DSS) rate is 93%. By multivariate analysis, an age of >45 years, the male gender and locally recurrent disease are predictive of a poor 5-year DSS rate. For metastatic disease, the outcome is poor, with a 5-year DSS rate of 8.2%. In comparison with other sarcomas, MLPS often metastasizes to the abdomen (49%), rather than the lungs (14%) and bones (23%) (3). Understanding the metastasis pattern and underlying mechanism should aid in improving the survival of patients with this disease.
The current study presents a rare case of MLPS with multiple metastases in fat-bearing areas, but no involvement of the lungs. Unexpectedly, the presence of bone metastasis as diagnosed by magnetic resonance imaging (MRI) and proven by histology, was negative on bone scans. Written informed consent was obtained from the patient's family.
Case report
A 53-year-old male presented with a slowly enlarging mass in the right thigh in May 2008. The patient underwent a local excision at Shanghai General Hospital (Shanghai, China). and the histology revealed a diagnosis of MLPS. The patient subsequently received adjuvant radiotherapy (details unknown) and was free from recurrence for 34 months after the resection. The patient presented to The Sixth People's Hospital of Shanghai Jiao Tong University (Shanghai, China) on April 26, 2011, with lower back pain that radiated to the bilateral thighs for more than one month. Bone scans showed no evidence of bone metastasis. However, MRI showed abnormal signals in the vertebral body of T11 and T12, and a mass in the epidural region at the T11 and T12 vertebral level (Fig. 1). Angiography showed that the feeding vessels were the T11 and T12 intercostal arteries. Transcatheter arterial embolization was performed using polyvinylpyrrolidone and gelfoam particles, and the contrast extravasation almost disappeared in the two arteries. Next day, a laminectomy was performed and the soft tumor in the epidural region was marginally resected. Immunohistochemically, the tumor cells were positive for vimentin, slightly positive for Ki-67 (2%), and negative for cluster of differentiation (CD)31, CD34, S-100, smooth muscle actin, glial fibrillary acidic protein and CD56. These findings together with hematoxylin and eosin staining revealed a diagnosis of metastatic MLPS for the involved bones and the mass in the epidural region.
Following recovery from the surgery, radiotherapy was administered at 48 Gy for 24 fractions over 5 weeks. At 1 week after the initiation of radiotherapy, concurrent chemotherapy consisting of gemcitabine (0.8 g/m2 on days 1 and 8) and docetaxel (75 mg/m2 on day 8) was administered. The adverse events were mild, such as leukopenia of World Health Organization grade I (4). At 16 days post-radiotherapy, a second course of chemotherapy with the same regimen was administered. Another 18 days later, computed tomography (CT) detected a 4.5×3.7-cm mass in the abdominal cavity below the gastric body. Several days later, the patient started complaining of blurred vision and painless diplopia in the left eye. Proptosis of the left eye became progressively evident. MRI showed a retrobulbar mass, which was isointense on T1-weighted images and hypo- to isointense on T2-weighted images, with ring enhancement following gadolinium-DTPA infusion (Fig. 2). The mass slightly compressed the optic nerve. The diagnosis was of an orbital metastasis. To alleviate the eye symptoms, the patient received palliative radiotherapy (21 fractions of 51 Gy over 10 weeks) to the retrobulbar mass. During the radiotherapy, the patient developed multiple metastases in regions that included the pelvic cavity, the pelvis femur and the lumbar vertebrae, and progression of the orbital mass was observed (Fig. 3). However, there was no involvement of the lungs. Although the patient received second-line chemotherapy with ifosfamide (1.8 g/m2 on days 1 to 5) and epirubicin (60 mg/m2 on days 1 and 2), the disease progressed and the patient's performance state deteriorated. The patient succumbed to the disease 3 months later.
Discussion
Metastasis to the orbit from LPS is rare and only four previous reports can be found in a search of the literature (5–8). The primary tumor locations were the retroperitoneum, the thigh and the abdominal cavity, and the pathological types were low/intermediate-differentiated LPS, MLPS, spindle-cell LPS or dedifferentiated LPS, respectively. Irrespective of the palliative surgical and systemic treatment, the prognosis of patients with orbit metastasis is poor.
Metastasis to the epidural region from LPS is also rare. Only three cases have been documented in the literature. The histological type of these three cases was uniformly MLPS. One case was in the cervical region and presented with liver, subcutaneous tissue metastasis (9). The second case was in the thoracic region and the patient had a history of lymph node, left buttock and left thoracic wall metastases. The patient succumbed to multiple lung metastases a few months later (10). The third case was in the thoracic region, and the patient developed abdominal metastasis and succumbed 7 weeks after the laminectomy (10). In the present case, the patient developed spinal metastases and progression with abdominal and orbital metastases 4 months after laminectomy. Collectively, these cases may suggest that epidural metastasis tends to arise from MLPS. Since the disease progressed rapidly in the patients with epidural metastasis, careful follow-up is strongly recommended to rule out metastasis in other locations.
Metastases from sarcoma are found mainly in the lungs and bones. LPS, which is the most common type of sarcoma, has an extrapulmonary metastasis pattern. The data from a study of 45 patients with MLPS suggested that the most common sites of spread were the bones, intraabdominal region, skin, mediastinum, paraspinal region and pleura (11). Cheng et al (12) also found that LPS has a tendency towards metastases occurring in the abdominal wall, axilla, breasts, subcutis, liver, dura mater, thymus and intraabdominal region. This distinct metastasis pattern was also proved by Hoffman et al (4) in a recent large-scale study of MLPS. Collectively, these results suggest that LPS, particularly MLPS, has a tendency to metastasize to fat-bearing areas (10). Concordant with this literature, the present case exhibited metastases in fat-bearing areas, including epidural, bone, abdominal and orbital metastases. However, the reason for this tendency is not clear. Hoffman et al (4) completed a pilot study concerning the molecular characteristics of MLPS. The study showed that MLPS expressed high levels of adipophilin, peroxisome proliferator-activated receptor-γ (PPAR-γ), chemokine (C-X-C motif) receptor 4 (CXCR4), AXL receptor tyrosine kinase and platelet-derived growth factor receptor-β (PDGFR-β). The aforementioned molecules were correlated with adipogenesis, migration, invasion, angiogenesis and metastasis. Further investigation is warranted to determine whether adipophilin and CXCR4 molecules contributed to the unique metastatic pattern.
In the present case, spinal metastasis was detected by MRI, although bone scans were negative. Bone scans normally detect skeletal metastasis using technetium-99m methylene diphosphonate binding to osteoblastic cells. For the majority of other cancer types, it is a routine procedure for patients during follow-up screening, as it is more sensitive than plain radiographs and CT for finding skeletal metastasis. However, for MLPS, several previous studies reported that skeletal metastasis was detectable by MRI, but not by bone scans (13–17). In one previous study, based on whole-body MRI findings, 33 MLPS patients were identified with spinal metastases. These metastases were detected by bone scans in 16% of patients and by positron emission tomography scans in 14% of patients (1). The reason why bone scanning is not a reliable method to detect skeletal metastasis remains unclear. We hypothesize one possible reason for this may be that there are few osteoblastic cells in skeletal metastasis. Recently, a variant chromosomal translocation t(12;22)(q13;q12) was identified. This translocation results in the production of the Ewing sarcoma breakpoint region 1-DNA damage-inducible transcript 3 (EWSR1-DDIT3) fusion protein. This fusion protein exerted a selective effect on the transcriptional activity of cell lineage-specific marker genes in multipotent mesenchymal C3H10T1/2 cells. The osteoblastic marker Opn promoter was repressed, while the adipocytic marker PPAR-γ2 promoter was not affected. The study concluded that EWSR1-DDIT3 may contribute to the phenotypic selection of mesenchymal cells during MLPS initiation and development (18). We propose that by the effect of this protein, mesenchymal cells in skeletal metastasis mainly transformed to adipocyte-related cells, but few osteoblastic cells. Therefore, this histological change may result in ineffective detection of the skeletal metastasis by bone scans.
In summary, a case of MLPS with skeletal, epidural, orbital and abdominal cavity metastases was reported. MRI clearly showed an abnormal signal in the vertebral body, however, bone scans were negative. Recent results have suggested that MLPS expresses high levels of adipophilin, PPAR-γ, CXCR4, AXL receptor tyrosine kinase, PDGFR-β and may present with the EWSR1-DDIT3 fusion transcript. We hypothesize that these genetic and molecular changes may contribute to the distinct biological characteristics of MLPS.
References
Schwab JH, Boland PJ, Antonescu C, Bilsky MH and Healey JH: Spinal metastases from myxoid liposarcoma warrant screening with magnetic resonance imaging. Cancer. 110:1815–1822. 2007. View Article : Google Scholar : PubMed/NCBI | |
Sanfilippo R, Dei Tos AP and Casali PG: Myxoid liposarcoma and the mammalian target of rapamycin pathway. Curr Opin Oncol. 25:379–383. 2013. View Article : Google Scholar : PubMed/NCBI | |
Hoffman A, Ghadimi MP, Demicco EG, Creighton CJ, Torres K, Colombo C, Peng T, Lusby K, Ingram D, Hornick JL, et al: Localized and metastatic myxoid/round cell liposarcoma: Clinical and molecular observations. Cancer. 119:1868–1877. 2013. View Article : Google Scholar : PubMed/NCBI | |
Miller AB, Hoogstraten B, Staquet M and Winkler A: Reporting results of cancer treatment. Cancer. 47:207–214. 1981. View Article : Google Scholar : PubMed/NCBI | |
Fabi A, Salesi N, Vidiri A, Mirri A, Ferraresi V and Cognetti F: Retroperitoneal liposarcoma with metastasis to both orbits: An unusual metastatic site. Anticancer Res. 25:4769–4771. 2005.PubMed/NCBI | |
Abdalla MI, Ghaly AF and Hosni F: Liposarcoma with orbital metastases. Case report. Br J Ophthalmol. 50:426–428. 1966. View Article : Google Scholar : PubMed/NCBI | |
Tehrani AH, Heegaard S, Prause JU, Fledelius HC and Daugaard S: Liposarcoma metastatic to the orbit. Eur J Ophthalmol. 13:108–112. 2003.PubMed/NCBI | |
Fezza J and Sinard J: Metastatic liposarcoma to the orbit. Am J Ophthalmol. 123:271–272. 1997. View Article : Google Scholar : PubMed/NCBI | |
Lee SY, Kim HJ, Park SY, Park YH and Chung SK: Myxoid liposarcoma involving the liver, subcutaneous tissue and epidural space in a polycystic disease patient. Clin Nucl Med. 33:507–509. 2008. View Article : Google Scholar : PubMed/NCBI | |
Ogose A, Hotta T, Inoue Y, Sakata S, Takano R and Yamamura S: Myxoid liposarcoma metastatic to the thoracic epidural space without bone involvement: Report of two cases. Jpn J Clin Oncol. 31:447–449. 2001. View Article : Google Scholar : PubMed/NCBI | |
Fuglø HM, Maretty-Nielsen K, Hovgaard D, Keller JØ, Safwat AA and Petersen MM: Metastatic pattern, local relapse and survival of patients with myxoid liposarcoma: A retrospective study of 45 patients. Sarcoma. 2013:548–628. 2013. View Article : Google Scholar | |
Cheng EY, Springfield DS and Mankin HJ: Frequent incidence of extrapulmonary sites of initial metastasis in patients with liposarcoma. Cancer. 75:1120–1127. 1995. View Article : Google Scholar : PubMed/NCBI | |
Sakamoto A, Fukutoku Y, Matsumoto Y, Harimaya K, Oda Y and Iwamoto Y: Myxoid liposarcoma with negative features on bone scan and 18F-2-fluoro-2-deoxy-D-glucose-positron emission tomography. World J Surg Oncol. 10:2142012. View Article : Google Scholar : PubMed/NCBI | |
Conill C, Setoain X, Colomo L, Palacín A, Combalia-Aleu A, Pomés J, Marruecos J, Vargas M and Maurel J: Diagnostic efficacy of bone scintigraphy, magnetic resonance imaging and positron emission tomography in bone metastases of myxoid liposarcoma. J Magn Reson Imaging. 27:625–628. 2008. View Article : Google Scholar : PubMed/NCBI | |
Khurana JS, Rosenthal DI, Rosenberg AE and Mankin HJ: Skeletal metastases in liposarcoma detectable only by magnetic resonance imaging. Clin Orthop Relat Res. 204–207. 1989.PubMed/NCBI | |
Ishii T, Ueda T, Myoui A, Tamai N, Hosono N and Yoshikawa H: Unusual skeletal metastases from myxoid liposarcoma only detectable by MR imaging. Eur Radiol. 4:L185–L191. 2003. View Article : Google Scholar | |
Kato S, Kawahara N, Murakami H, Demura S, Shirai T, Tsuchiya H and Tomita K: Multi-level total en bloc spondylectomy for solitary lumbar metastasis of myxoid liposarcoma. Orthopedics. 33:4462010.PubMed/NCBI | |
Suzuki K, Matsui Y, Higashimoto M, Kawaguchi Y, Seki S, Motomura H, Hori T, Yahara Y, Kanamori M and Kimura T: Myxoid liposarcoma-associated EWSR1-DDIT3 selectively represses osteoblastic and chondrocytic transcription in multipotent mesenchymal cells. PLoS One. 7:e366822012. View Article : Google Scholar : PubMed/NCBI |