Open Access

Gene expression profiling analysis of MENX-associated rat pituitary adenomas contributes to understand molecular mechanisms of human pituitary adenomas

  • Authors:
    • Hongzhi Zhang
    • Chuan Xu
    • Ningyang Sun
    • Yinting Zhou
    • Xiaofei Yu
    • Xue Yan
    • Qiujuan Zhang
  • View Affiliations

  • Published online on: November 10, 2015     https://doi.org/10.3892/ol.2015.3904
  • Pages: 125-133
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to screen potential genes associated with pituitary adenomas to obtain further understanding with regard to the pathogenesis of pituitary adenomas. The microarray GSE23207 dataset, containing 16 pituitary adenoma samples from multiple endocrine neo­plasia syndrome‑associated rats and 5 normal pituitary tissue samples, was downloaded from Gene Expression Omnibus. The Linear Models for Microarray Data package was used to identify the differentially‑expressed genes (DEGs) with the cut‑off criteria of a |log2fold change (FC)|>1 and adjusted P‑values of <0.05. The potential functions of the DEGs were predicted by functional and pathway enrichment analysis with the Database for Annotation, Visualization and Integrated Discovery. Furthermore, the interaction associations of the up‑ and downregulated DEGs obtained from the Search Tool for the Retrieval of Interacting Genes database were respectively revealed by the protein‑protein interaction networks visualized with Cytoscape. A total of 391 upregulated and 238 downregulated DEGs in were screened in the pituitary adenoma samples. The upregulated DEGs with a higher degree in the protein‑protein interaction network (e.g., CCNA2, CCNB1 and CDC20) were significantly involved in cell cycle and cell division. Notably, PTTG1 was enriched in every functional term. These DEGs interacted with each other. The downregulated DEGs (e.g., GABRA1, GABRA4 and GABRB1) also interacted with each other, and were relevant to neuroactive ligand‑receptor interaction; the DEG POU1F1, interacting with POMC, was correlated with the development of the pituitary gland, adenohypophysis and endocrine system. Certain DEGs, including CCNB1, CCNA2, CDC20, GABRA1, GABRA4, GABRB1, POU1F1 and POMC, and particularly PTTG1, were shown to be closely involved in the pathogenesis of pituitary adenomas.
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January-2016
Volume 11 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zhang H, Xu C, Sun N, Zhou Y, Yu X, Yan X and Zhang Q: Gene expression profiling analysis of MENX-associated rat pituitary adenomas contributes to understand molecular mechanisms of human pituitary adenomas. Oncol Lett 11: 125-133, 2016
APA
Zhang, H., Xu, C., Sun, N., Zhou, Y., Yu, X., Yan, X., & Zhang, Q. (2016). Gene expression profiling analysis of MENX-associated rat pituitary adenomas contributes to understand molecular mechanisms of human pituitary adenomas. Oncology Letters, 11, 125-133. https://doi.org/10.3892/ol.2015.3904
MLA
Zhang, H., Xu, C., Sun, N., Zhou, Y., Yu, X., Yan, X., Zhang, Q."Gene expression profiling analysis of MENX-associated rat pituitary adenomas contributes to understand molecular mechanisms of human pituitary adenomas". Oncology Letters 11.1 (2016): 125-133.
Chicago
Zhang, H., Xu, C., Sun, N., Zhou, Y., Yu, X., Yan, X., Zhang, Q."Gene expression profiling analysis of MENX-associated rat pituitary adenomas contributes to understand molecular mechanisms of human pituitary adenomas". Oncology Letters 11, no. 1 (2016): 125-133. https://doi.org/10.3892/ol.2015.3904