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Article Open Access

A novel AKT inhibitor, AZD5363, inhibits phosphorylation of AKT downstream molecules, and activates phosphorylation of mTOR and SMG‑1 dependent on the liver cancer cell type

  • Authors:
    • Yuncheng Zhang
    • Yuanwen Zheng
    • Ali Faheem
    • Tiantong Sun
    • Chunyou Li
    • Zhe Li
    • Diantang Zhao
    • Chao Wu
    • Jun Liu
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery and Center of Organ Transplantation, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China, Shandong University School of Medicine, Jinan, Shandong 250021, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1685-1692
    |
    Published online on: January 14, 2016
       https://doi.org/10.3892/ol.2016.4111
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Abstract

Due to frequent phosphoinositide 3‑kinase (PI3K)/AKT/mammalian target of rapamycin (mTOR) signaling pathway dysregulation, AKT is typically accepted as a promising anticancer therapeutic target. mTOR, in particular, represents a suitable therapeutic target for hepatocellular carcinoma, whilst suppressor with morphogenetic effect on genitalia family member‑1 (SMG‑1) is believed to serve a potential tumor suppressor role in human cancer. Despite SMG‑1 and mTOR belonging to the same PI3K‑related kinase family, the interactions between them are not yet fully understood. In the present study, a novel pyrrolopyrimidine‑derived compound, AZD5363, was observed to suppress proliferation in liver cancer Hep‑G2 and Huh‑7 cells by inhibiting the phosphorylation of downstream molecules in the AKT signal pathway, in a dose‑ and time‑dependent manner. AZD5363 activated the phosphorylation of mTOR, dependent on the liver cancer cell type, as it may have differing effects in various liver cancer cell lines. Additionally, AZD5363 also activated SMG‑1 within the same liver cancer cells types, which subsequently activated the phosphorylation of mTOR. In conclusion, the present study indicates that AZD5363 inhibited phosphorylation of AKT downstream molecules, and activated phosphorylation of mTOR and SMG‑1, dependent on the liver cancer type.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang Y, Zheng Y, Faheem A, Sun T, Li C, Li Z, Zhao D, Wu C and Liu J: A novel AKT inhibitor, AZD5363, inhibits phosphorylation of AKT downstream molecules, and activates phosphorylation of mTOR and SMG‑1 dependent on the liver cancer cell type. Oncol Lett 11: 1685-1692, 2016.
APA
Zhang, Y., Zheng, Y., Faheem, A., Sun, T., Li, C., Li, Z. ... Liu, J. (2016). A novel AKT inhibitor, AZD5363, inhibits phosphorylation of AKT downstream molecules, and activates phosphorylation of mTOR and SMG‑1 dependent on the liver cancer cell type. Oncology Letters, 11, 1685-1692. https://doi.org/10.3892/ol.2016.4111
MLA
Zhang, Y., Zheng, Y., Faheem, A., Sun, T., Li, C., Li, Z., Zhao, D., Wu, C., Liu, J."A novel AKT inhibitor, AZD5363, inhibits phosphorylation of AKT downstream molecules, and activates phosphorylation of mTOR and SMG‑1 dependent on the liver cancer cell type". Oncology Letters 11.3 (2016): 1685-1692.
Chicago
Zhang, Y., Zheng, Y., Faheem, A., Sun, T., Li, C., Li, Z., Zhao, D., Wu, C., Liu, J."A novel AKT inhibitor, AZD5363, inhibits phosphorylation of AKT downstream molecules, and activates phosphorylation of mTOR and SMG‑1 dependent on the liver cancer cell type". Oncology Letters 11, no. 3 (2016): 1685-1692. https://doi.org/10.3892/ol.2016.4111
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Y, Zheng Y, Faheem A, Sun T, Li C, Li Z, Zhao D, Wu C and Liu J: A novel AKT inhibitor, AZD5363, inhibits phosphorylation of AKT downstream molecules, and activates phosphorylation of mTOR and SMG‑1 dependent on the liver cancer cell type. Oncol Lett 11: 1685-1692, 2016.
APA
Zhang, Y., Zheng, Y., Faheem, A., Sun, T., Li, C., Li, Z. ... Liu, J. (2016). A novel AKT inhibitor, AZD5363, inhibits phosphorylation of AKT downstream molecules, and activates phosphorylation of mTOR and SMG‑1 dependent on the liver cancer cell type. Oncology Letters, 11, 1685-1692. https://doi.org/10.3892/ol.2016.4111
MLA
Zhang, Y., Zheng, Y., Faheem, A., Sun, T., Li, C., Li, Z., Zhao, D., Wu, C., Liu, J."A novel AKT inhibitor, AZD5363, inhibits phosphorylation of AKT downstream molecules, and activates phosphorylation of mTOR and SMG‑1 dependent on the liver cancer cell type". Oncology Letters 11.3 (2016): 1685-1692.
Chicago
Zhang, Y., Zheng, Y., Faheem, A., Sun, T., Li, C., Li, Z., Zhao, D., Wu, C., Liu, J."A novel AKT inhibitor, AZD5363, inhibits phosphorylation of AKT downstream molecules, and activates phosphorylation of mTOR and SMG‑1 dependent on the liver cancer cell type". Oncology Letters 11, no. 3 (2016): 1685-1692. https://doi.org/10.3892/ol.2016.4111
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