Assessment of ALK gene fusions in lung cancer using the differential expression and exon integrity methods

  • Authors:
    • Qing Huang
    • Qiuhua Deng
    • Lei Jiang
    • Rong Fang
    • Yinghua Qiu
    • Ping Wang
    • Jeff X. Zhou
    • Haihong Yang
  • View Affiliations

  • Published online on: January 27, 2016     https://doi.org/10.3892/ol.2016.4157
  • Pages: 1651-1656
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Abstract

Anaplastic lymphoma kinase (ALK) gene fusion is a driving mutation underlying the development of non‑small cell lung cancer (NSCLC). Accurate detection of ALK fusion is critical for the use of ALK inhibitors in the treatment of NSCLC. Commonly utilized methods for ALK detection include fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC). However, these methods are time‑consuming and costly. In the present study, a method for assessing ALK gene fusion based on the differential expression levels of the ALK kinase and non‑kinase domains was developed and evaluated, with the aim of providing a convenient and reliable method for the detection of ALK fusion. In addition, another method was established to determine the integrity of exons 19‑20 and 20‑21 of ALK, two genomic loci that are typically broken in ALK fusions. These novel methods were applied to detect ALK fusion in 100 NSCLC patients, and were compared with IHC and FISH methods. The novel methods developed in the present study successfully detected ALK fusions in 10 samples. The concordances between the novel methods and IHC and FISH were 100%. Furthermore, the differential expression method was able to detect ALK fusions in cell‑free urine samples, which was advantageous over FISH and IHC. The novel methods developed in the present study are cost‑effective and easy to perform, and may provide simple and convenient techniques for the clinical assessment of ALK fusions, facilitating the use of targeted therapy for NSCLC.
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March-2016
Volume 11 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Huang Q, Deng Q, Jiang L, Fang R, Qiu Y, Wang P, Zhou JX and Yang H: Assessment of ALK gene fusions in lung cancer using the differential expression and exon integrity methods. Oncol Lett 11: 1651-1656, 2016
APA
Huang, Q., Deng, Q., Jiang, L., Fang, R., Qiu, Y., Wang, P. ... Yang, H. (2016). Assessment of ALK gene fusions in lung cancer using the differential expression and exon integrity methods. Oncology Letters, 11, 1651-1656. https://doi.org/10.3892/ol.2016.4157
MLA
Huang, Q., Deng, Q., Jiang, L., Fang, R., Qiu, Y., Wang, P., Zhou, J. X., Yang, H."Assessment of ALK gene fusions in lung cancer using the differential expression and exon integrity methods". Oncology Letters 11.3 (2016): 1651-1656.
Chicago
Huang, Q., Deng, Q., Jiang, L., Fang, R., Qiu, Y., Wang, P., Zhou, J. X., Yang, H."Assessment of ALK gene fusions in lung cancer using the differential expression and exon integrity methods". Oncology Letters 11, no. 3 (2016): 1651-1656. https://doi.org/10.3892/ol.2016.4157