MicroRNA‑497 suppresses osteosarcoma tumor growth in vitro and in vivo

Ge,Liang; Zheng,Baisong; Li,Minghe; Niu,Liang; Li,Zhihong

doi:10.3892/ol.2016.4162

MicroRNA‑497 suppresses osteosarcoma tumor growth in vitro and in vivo

Authors:
- Liang Ge
- Baisong Zheng
- Minghe Li
- Liang Niu
- Zhihong Li
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Affiliations: Department of Anesthesiology, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Institute of Virology and AIDS Research, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Department of Oral and Maxillofacial Surgery, School of Stomatology Hospital of Jilin University, Changchun, Jilin 130000, P.R. China, Operating Room, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Department of Thoracic Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China
Published online on: January 29, 2016 https://doi.org/10.3892/ol.2016.4162
Pages: 2207-2212

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Abstract

It has been demonstrated that microRNA‑497 (miR‑497) acts as a tumor suppressor and is involved in tumor progression, development and metastasis in several types of cancer. However, little is known about the exact role of miR‑497 in osteosarcoma (OS). The aim of the current study was to investigate the potential role of miR‑497 in human OS. The role of miR‑497 in the growth and survival of OS cells was determined using several in vitro approaches and a nude mouse model. The results demonstrated that exogenous expression of miR‑497 in human OS MG63 cells suppressed cell proliferation, colony formation, migration and invasion, and induced cell apoptosis and cell arrest at the G0/G1 phase of the cell cycle. In addition, the results of the in vivo study indicated that restoration of miR‑497 inhibited OS tumor growth in a nude mouse model. Overall, the results of the present study identified a crucial tumor suppressive role of miR‑497 in the progression of OS, and suggested that miR‑497 may be a potential therapeutic agent for the treatment of OS.

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