Upregulation of PREX2 promotes the proliferation and migration of hepatocellular carcinoma cells via PTEN‑AKT signaling

  • Authors:
    • Shujie He
    • Juan Lin
    • Shaoping Yu
    • Shijie Sun
  • View Affiliations

  • Published online on: January 29, 2016     https://doi.org/10.3892/ol.2016.4164
  • Pages: 2223-2228
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Phosphatidylinositol‑3,4,5‑trisphosphate Rac exchanger 2 (PREX2), a regulator of the small guanosine triphosphatase Rac, demonstrates an inhibitory effect on the activity of phosphatase and tensin homolog (PTEN). Previously, PREX2 was implicated in the regulation of cell invasion in hepatocellular carcinoma (HCC). However, the exact role of PREX2 in the regulation of HCC cell proliferation and migration, as well as the underlying mechanisms, remains unclear. In the present study, reverse transcription‑quantitative polymerase chain reaction revealed that PREX2 was upregulated in HCC tissue compared with matched adjacent non‑tumorous tissue. In addition, the present study demonstrated that the messenger RNA and protein levels of PREX2 increased in human HCC HepG2, LH86, LMH and PLHC‑1 cell lines compared with normal human liver THLE‑3 cells. Overexpression of PREX2 significantly enhanced the proliferation and migration of HCC cells, and knockdown of PREX2 expression significantly suppressed the proliferation and migration of HCC cells. Additional investigation revealed that overexpression of PREX2 suppressed the activity of PTEN, leading to an enhancement in the activity of protein kinase B (AKT). By contrast, knockdown of PREX2 expression upregulated the activity of PTEN and suppressed the activity of AKT. Overall, the present study suggests that PREX2 promotes the proliferation and migration of HCC cells by inhibiting PTEN‑AKT signaling.
View Figures
View References

Related Articles

Journal Cover

March-2016
Volume 11 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
He S, Lin J, Yu S and Sun S: Upregulation of PREX2 promotes the proliferation and migration of hepatocellular carcinoma cells via PTEN‑AKT signaling. Oncol Lett 11: 2223-2228, 2016.
APA
He, S., Lin, J., Yu, S., & Sun, S. (2016). Upregulation of PREX2 promotes the proliferation and migration of hepatocellular carcinoma cells via PTEN‑AKT signaling. Oncology Letters, 11, 2223-2228. https://doi.org/10.3892/ol.2016.4164
MLA
He, S., Lin, J., Yu, S., Sun, S."Upregulation of PREX2 promotes the proliferation and migration of hepatocellular carcinoma cells via PTEN‑AKT signaling". Oncology Letters 11.3 (2016): 2223-2228.
Chicago
He, S., Lin, J., Yu, S., Sun, S."Upregulation of PREX2 promotes the proliferation and migration of hepatocellular carcinoma cells via PTEN‑AKT signaling". Oncology Letters 11, no. 3 (2016): 2223-2228. https://doi.org/10.3892/ol.2016.4164