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Article

Upregulation of PREX2 promotes the proliferation and migration of hepatocellular carcinoma cells via PTEN‑AKT signaling

  • Authors:
    • Shujie He
    • Juan Lin
    • Shaoping Yu
    • Shijie Sun
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery, Yantai Mountain Hospital, Yantai, Shandong 264000, P.R. China, Department of Hepatobiliary Surgery, Government Hospital of Yantai City, Yantai, Shandong 264000, P.R. China, Department of Hepatobiliary Surgery, Yuhuangding Hospital, Yantai, Shandong 264001, P.R. China
  • Pages: 2223-2228
    |
    Published online on: January 29, 2016
       https://doi.org/10.3892/ol.2016.4164
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Abstract

Phosphatidylinositol‑3,4,5‑trisphosphate Rac exchanger 2 (PREX2), a regulator of the small guanosine triphosphatase Rac, demonstrates an inhibitory effect on the activity of phosphatase and tensin homolog (PTEN). Previously, PREX2 was implicated in the regulation of cell invasion in hepatocellular carcinoma (HCC). However, the exact role of PREX2 in the regulation of HCC cell proliferation and migration, as well as the underlying mechanisms, remains unclear. In the present study, reverse transcription‑quantitative polymerase chain reaction revealed that PREX2 was upregulated in HCC tissue compared with matched adjacent non‑tumorous tissue. In addition, the present study demonstrated that the messenger RNA and protein levels of PREX2 increased in human HCC HepG2, LH86, LMH and PLHC‑1 cell lines compared with normal human liver THLE‑3 cells. Overexpression of PREX2 significantly enhanced the proliferation and migration of HCC cells, and knockdown of PREX2 expression significantly suppressed the proliferation and migration of HCC cells. Additional investigation revealed that overexpression of PREX2 suppressed the activity of PTEN, leading to an enhancement in the activity of protein kinase B (AKT). By contrast, knockdown of PREX2 expression upregulated the activity of PTEN and suppressed the activity of AKT. Overall, the present study suggests that PREX2 promotes the proliferation and migration of HCC cells by inhibiting PTEN‑AKT signaling.
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Copy and paste a formatted citation
Spandidos Publications style
He S, Lin J, Yu S and Sun S: Upregulation of PREX2 promotes the proliferation and migration of hepatocellular carcinoma cells via PTEN‑AKT signaling. Oncol Lett 11: 2223-2228, 2016.
APA
He, S., Lin, J., Yu, S., & Sun, S. (2016). Upregulation of PREX2 promotes the proliferation and migration of hepatocellular carcinoma cells via PTEN‑AKT signaling. Oncology Letters, 11, 2223-2228. https://doi.org/10.3892/ol.2016.4164
MLA
He, S., Lin, J., Yu, S., Sun, S."Upregulation of PREX2 promotes the proliferation and migration of hepatocellular carcinoma cells via PTEN‑AKT signaling". Oncology Letters 11.3 (2016): 2223-2228.
Chicago
He, S., Lin, J., Yu, S., Sun, S."Upregulation of PREX2 promotes the proliferation and migration of hepatocellular carcinoma cells via PTEN‑AKT signaling". Oncology Letters 11, no. 3 (2016): 2223-2228. https://doi.org/10.3892/ol.2016.4164
Copy and paste a formatted citation
x
Spandidos Publications style
He S, Lin J, Yu S and Sun S: Upregulation of PREX2 promotes the proliferation and migration of hepatocellular carcinoma cells via PTEN‑AKT signaling. Oncol Lett 11: 2223-2228, 2016.
APA
He, S., Lin, J., Yu, S., & Sun, S. (2016). Upregulation of PREX2 promotes the proliferation and migration of hepatocellular carcinoma cells via PTEN‑AKT signaling. Oncology Letters, 11, 2223-2228. https://doi.org/10.3892/ol.2016.4164
MLA
He, S., Lin, J., Yu, S., Sun, S."Upregulation of PREX2 promotes the proliferation and migration of hepatocellular carcinoma cells via PTEN‑AKT signaling". Oncology Letters 11.3 (2016): 2223-2228.
Chicago
He, S., Lin, J., Yu, S., Sun, S."Upregulation of PREX2 promotes the proliferation and migration of hepatocellular carcinoma cells via PTEN‑AKT signaling". Oncology Letters 11, no. 3 (2016): 2223-2228. https://doi.org/10.3892/ol.2016.4164
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