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Article

MiR-593 mediates curcumin-induced radiosensitization of nasopharyngeal carcinoma cells via MDR1

  • Authors:
    • Haoning Fan
    • Meng Shao
    • Shaohui Huang
    • Ying Liu
    • Jie Liu
    • Zhiyuan Wang
    • Jianxin Diao
    • Yuanliang Liu
    • Li Tong
    • Qin Fan
  • View Affiliations / Copyright

    Affiliations: Department of Molecular Biology, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of Radiotherapy, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China
  • Pages: 3729-3734
    |
    Published online on: April 14, 2016
       https://doi.org/10.3892/ol.2016.4438
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Abstract

Curcumin (Cur) exhibits radiosensitization effects to a variety of malignant tumors. The present study investigates the radiosensitizing effect of Cur on nasopharyngeal carcinoma (NPC) cells and whether its mechanism is associated with microRNA‑593 (miR‑593) and multidrug resistance gene 1 (MDR1). A clonogenic assay was performed to measure the radiosensitizing effect. The expression of miR‑593 and MDR1 was analyzed by quantitative polymerase chain reaction (qPCR) or western blot assay. A transplanted tumor model was established to identify the radiosensitizing effect in vivo. A luciferase-based reporter was constructed to evaluate the effect of direct binding of miR‑593 to the putative target site on the 3' UTR of MDR1. The clonogenic assay showed that Cur enhanced the radiosensitivity of cells. Cur (100 mg/kg) combined with 4 Gy irradiation inhibited the growth of a transplanted tumor model in vivo, resulting in the higher inhibition ratio compared with the radiotherapy‑alone group. These results demonstrated that Cur had a radiosensitizing effect on NPC cells in vivo and in vitro; Cur‑mediated upregulation of miR‑593 resulted in reduced MDR1 expression, which may promote radiosensitivity of NPC cells.
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1 

Loong HH, Ma BB and Chan AT: Update on the management and therapeutic monitoring of advanced nasopharyngeal cancer. Hematol Oncol Clin North Am. 22:1267–1278. 2008. View Article : Google Scholar : PubMed/NCBI

2 

Chan AT, Teo PM and Johnson PJ: Nasopharyngeal carcinoma. Ann Oncol. 13:1007–1015. 2002. View Article : Google Scholar : PubMed/NCBI

3 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar : PubMed/NCBI

4 

Sanguineti G, Geara FB, Garden AS, Tucker SL, Ang KK, Morrison WH and Peters LJ: Carcinoma of the nasopharynx treated by radiotherapy alone: Determinants of local and regional control. Int J Radiat Oncol Biol Phys. 37:985–996. 1997. View Article : Google Scholar : PubMed/NCBI

5 

Siegel R, Naishadham D and Jemal A: Cancer statistics, 2013. CA Cancer J Clin. 63:11–30. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Ji JL, Huang XF and Zhu HL: Curcumin and its formulations: Potential anti-cancer agents. Anticancer Agents Med Chem. 12:210–218. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Rahmani AH, Al Zohairy MA, Aly SM and Khan MA: Curcumin: A potential candidate in prevention of cancer via modulation of molecular pathways. Biomed Res Int. 2014:7616082014. View Article : Google Scholar : PubMed/NCBI

8 

López-Jornet P, Camacho-Alonso F and Gómez-Garcia F: Effect of curcumin and irradiation in PE/CA-PJ15 oral squamous cell carcinoma. Acta Odontol Scand. 69:269–273. 2011. View Article : Google Scholar : PubMed/NCBI

9 

Chang YJ, Huang CY, Hung CS, Chen WY and Wei PL: GRP78 mediates the therapeutic efficacy of curcumin on colon cancer. Tumour Biol. 36:633–641. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Xie YQ, Wu XB and Tang SQ: Curcumin treatment alters ERK-1/2 signaling in vitro and inhibits nasopharyngeal carcinoma proliferation in mouse xenografts. Int J Clin Exp Med. 7:108–114. 2014.PubMed/NCBI

11 

Liu Y, Cai H, Liu J, Fan H, Wang Z, Wang Q, Shao M, Sun X, Diao J, Liu Y, et al: A miR-151 binding site polymorphism in the 3′-untranslated region of the cyclin E1 gene associated with nasopharyngeal carcinoma. Biochem Biophys Res Commun. 432:660–665. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Wang Q, Fan H, Liu Y, Yin Z, Cai H, Liu J, Wang Z, Shao M, Sun X, Diao J, et al: Curcumin enhances the radiosensitivity in nasopharyngeal carcinoma cells involving the reversal of differentially expressed long non-coding RNAs. Int J Oncol. 44:858–864. 2014.PubMed/NCBI

13 

Djuranovic S, Nahvi A and Green R: A parsimonious model for gene regulation by miRNAs. Science. 331:550–553. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Yang B, Jing C, Wang J, Guo X, Chen Y, Xu R, Peng L, Liu J and Li L: Identification of microRNAs associated with lymphangiogenesis in human gastric cancer. Clin Transl Oncol. 16:374–379. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Zhang T, Sun Q, Liu T, Chen J, Du S, Ren C, Liao G and Yuan Y: MiR-451 increases radiosensitivity of nasopharyngeal carcinoma cells by targeting ras-related protein 14 (RAB14). Tumour Biol. 35:12593–12599. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Nygren MK, Tekle C, Ingebrigtsen VA, Mäkelä R, Krohn M, Aure MR, Nunes-Xavier CE, Perälä M, Tramm T, Alsner J, et al: Identifying microRNAs regulating B7-H3 in breast cancer: The clinical impact of microRNA-29c. Br J Cancer. 110:2072–2080. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Ito T, Sato F, Kan T, Cheng Y, David S, Agarwal R, Paun BC, Jin Z, Olaru AV, Hamilton JP, et al: Polo-like kinase 1 regulates cell proliferation and is targeted by miR-593* in esophageal cancer. Int J Cancer. 129:2134–2146. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Zhang Y, Zheng L, Huang J, Gao F, Lin X, He L, Li D, Li Z, Ding Y and Chen L: MiR-124 Radiosensitizes human colorectal cancer cells by targeting PRRX1. PLoS One. 9:e939172014. View Article : Google Scholar : PubMed/NCBI

19 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2-∆∆CT method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

20 

Yaromina A, Krause M, Thames H, Rosner A, Krause M, Hessel F, Grenman R, Zips D and Baumann M: Pre-treatment number of clonogenic cells and their radiosensitivity are major determinants of local tumour control after fractionated irradiation. Radiother Oncol. 83:304–310. 2007. View Article : Google Scholar : PubMed/NCBI

21 

Lu ZX, Ma XQ, Yang LF, Wang ZL, Zeng L, Li ZJ, Li XN, Tang M, Yi W, Gong JP, et al: DNAzymes targeted to EBV-encoded latent membrane protein-1 induce apoptosis and enhance radiosensitivity in nasopharyngeal carcinoma. Cancer Lett. 265:226–238. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Pearce AG, Segura TM, Rintala AC, Rintala-Maki ND and Lee H: The generation and characterization of a radiation-resistant model system to study radioresistance in human breast cancer cells. Radiat Res. 156:739–750. 2001. View Article : Google Scholar : PubMed/NCBI

23 

Baldwin AS: Control of oncogenesis and cancer therapy resistance by the transcription factor NF-kappaB. J Clin Invest. 107:241–246. 2001. View Article : Google Scholar : PubMed/NCBI

24 

Kunnumakkara AB, Diagaradjane P, Guha S, Deorukhkar A, Shentu S, Aggarwal BB and Krishnan S: Curcumin sensitizes human colorectal cancer xenografts in nude mice to gamma-radiation by targeting nuclear factor-kappaB-regulated gene products. Clin Cancer Res. 14:2128–2136. 2008. View Article : Google Scholar : PubMed/NCBI

25 

Javvadi P, Segan AT, Tuttle SW and Koumenis C: The chemopreventive agent curcumin is a potent radiosensitizer of human cervical tumor cells via increased reactive oxygen species production and over activation of the mitogen-activated protein kinase pathway. Mol Pharmacol. 73:1491–1501. 2008. View Article : Google Scholar : PubMed/NCBI

26 

Narang H and Krishna M: Inhibition of radiation induced nitration by curcumin and nicotinamide in mouse macrophages. Mol Cell Biochem. 276:7–13. 2005. View Article : Google Scholar : PubMed/NCBI

27 

Hannoun-Levi JM, Chand-Fouche ME, Dejean C and Courdi A: Dose gradient impact on equivalent dose at 2 Gy for high dose rate interstitial brachytherapy. J Contemp Brachytherapy. 4:14–20. 2012. View Article : Google Scholar : PubMed/NCBI

28 

Montazami N, Aghapour M, Farajnia S and Baradaran B: New insights into the mechanisms of multidrug resistance in cancers. Cell Mol Biol (Noisy-le-grand). 61:70–80. 2015.PubMed/NCBI

29 

Maier P, Herskind C, Fleckenstein K, Spier I, Laufs S, Zeller WJ, Fruehauf S and Wenz F: MDR1 gene transfer using a lentiviral SIN vector confers radioprotection to human CD34+ hematopoietic progenitor cells. Radiat Res. 169:301–310. 2008. View Article : Google Scholar : PubMed/NCBI

30 

Oh JS, Kim JJ, Byun JY and Kim IA: Lin28-let7 modulates radiosensitivity of human cancer cells with activation of K-Ras. Int J Radiat Oncol Biol Phys. 76:5–8. 2010. View Article : Google Scholar : PubMed/NCBI

31 

van Jaarsveld MT, Wouters MD, Boersma AW, Smid M, van Ijcken WF, Mathijssen RH, Hoeijmakers JH, Martens JW, van Laere S, Wiemer EA and Pothof J: DNA damage responsive microRNAs misexpressed in human cancer modulate therapy sensitivity. Mol Oncol. 8:458–468. 2014. View Article : Google Scholar : PubMed/NCBI

32 

Sun Q, Liu T, Zhang T, Du S, Xie GX, Lin X, Chen L and Yuan Y: MiR-101 sensitizes human nasopharyngeal carcinoma cells to radiation by targeting stathmin 1. Mol Med Rep. 11:3330–3336. 2015.PubMed/NCBI

33 

Mansour WY, Bogdanova NV, Kasten-Pisula U, Rieckmann T, Köcher S, Borgmann K, Baumann M, Krause M, Petersen C, Hu H, et al: Aberrant overexpression of miR-421 downregulates ATM and leads to a pronounced DSB repair defect and clinical hypersensitivity in SKX squamous cell carcinoma. Radiother Oncol. 106:147–154. 2013. View Article : Google Scholar : PubMed/NCBI

34 

Ke G, Liang L, Yang JM, Huang X, Han D, Huang S, Zhao Y, Zha R, He X and Wu X: MiR-181a confers resistance of cervical cancer to radiation therapy through targeting the pro-apoptotic PRKCD gene. Oncogene. 32:3019–3027. 2013. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Fan H, Shao M, Huang S, Liu Y, Liu J, Wang Z, Diao J, Liu Y, Tong L, Fan Q, Fan Q, et al: MiR-593 mediates curcumin-induced radiosensitization of nasopharyngeal carcinoma cells via MDR1. Oncol Lett 11: 3729-3734, 2016.
APA
Fan, H., Shao, M., Huang, S., Liu, Y., Liu, J., Wang, Z. ... Fan, Q. (2016). MiR-593 mediates curcumin-induced radiosensitization of nasopharyngeal carcinoma cells via MDR1. Oncology Letters, 11, 3729-3734. https://doi.org/10.3892/ol.2016.4438
MLA
Fan, H., Shao, M., Huang, S., Liu, Y., Liu, J., Wang, Z., Diao, J., Liu, Y., Tong, L., Fan, Q."MiR-593 mediates curcumin-induced radiosensitization of nasopharyngeal carcinoma cells via MDR1". Oncology Letters 11.6 (2016): 3729-3734.
Chicago
Fan, H., Shao, M., Huang, S., Liu, Y., Liu, J., Wang, Z., Diao, J., Liu, Y., Tong, L., Fan, Q."MiR-593 mediates curcumin-induced radiosensitization of nasopharyngeal carcinoma cells via MDR1". Oncology Letters 11, no. 6 (2016): 3729-3734. https://doi.org/10.3892/ol.2016.4438
Copy and paste a formatted citation
x
Spandidos Publications style
Fan H, Shao M, Huang S, Liu Y, Liu J, Wang Z, Diao J, Liu Y, Tong L, Fan Q, Fan Q, et al: MiR-593 mediates curcumin-induced radiosensitization of nasopharyngeal carcinoma cells via MDR1. Oncol Lett 11: 3729-3734, 2016.
APA
Fan, H., Shao, M., Huang, S., Liu, Y., Liu, J., Wang, Z. ... Fan, Q. (2016). MiR-593 mediates curcumin-induced radiosensitization of nasopharyngeal carcinoma cells via MDR1. Oncology Letters, 11, 3729-3734. https://doi.org/10.3892/ol.2016.4438
MLA
Fan, H., Shao, M., Huang, S., Liu, Y., Liu, J., Wang, Z., Diao, J., Liu, Y., Tong, L., Fan, Q."MiR-593 mediates curcumin-induced radiosensitization of nasopharyngeal carcinoma cells via MDR1". Oncology Letters 11.6 (2016): 3729-3734.
Chicago
Fan, H., Shao, M., Huang, S., Liu, Y., Liu, J., Wang, Z., Diao, J., Liu, Y., Tong, L., Fan, Q."MiR-593 mediates curcumin-induced radiosensitization of nasopharyngeal carcinoma cells via MDR1". Oncology Letters 11, no. 6 (2016): 3729-3734. https://doi.org/10.3892/ol.2016.4438
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