Inhibitory effect of MyoD on the proliferation of breast cancer cells

Cai,Changjing; Qin,Xiaoqun; Wu,Ziyi; Shen,Qixia; Yang,Wenqian; Zhang,Shujun; Duan,Jinling; Liang,Fenglan; Liu,Chi

doi:10.3892/ol.2016.4448

Inhibitory effect of MyoD on the proliferation of breast cancer cells

Authors:
- Changjing Cai
- Xiaoqun Qin
- Ziyi Wu
- Qixia Shen
- Wenqian Yang
- Shujun Zhang
- Jinling Duan
- Fenglan Liang
- Chi Liu
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Affiliations: Physiology Department, Xiangya Medical School, Central South University, Changsha, Hunan 410078, P.R. China
Published online on: April 18, 2016 https://doi.org/10.3892/ol.2016.4448
Pages: 3589-3596
Copyright: © Cai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Skeletal muscle is rich in lymphatic vessels, with an abundant blood supply, and it is an infrequent site of cancer metastasis. Previous studies have demonstrated that enhanced secretion of MyoD may occur when skeletal muscle is injured or becomes cancerous. It was hypothesized that MyoD may act as an endogenous cytokine to inhibit the proliferation of cancer cells. To verify the possible effect of this protein on tumor cell proliferation, C2C12 mouse skeletal muscle cells and 4T1 mouse breast cancer cells were co‑cultured using embedded Transwell plates. Following co‑culture, cell cycle analysis revealed that C2C12 muscle cells were able to inhibit the proliferation of the breast cancer cells. Subsequently, MyoD was silenced in C2C12 cells to assess its effect on 4T1 cell proliferation. Following co‑culture with MyoD‑silenced cells, a 5‑ethynyl-20-deoxyuridine assay indicated that MyoD silencing prevented the reduction in proliferation of 4T1 cells induced by untransfected C2C12 cells. In summary, the results indicated that MyoD inhibits the proliferation of breast cancer cells and may be a tumor suppressor factor.

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