miR-494 inhibits ovarian cancer cell proliferation and promotes apoptosis by targeting FGFR2

  • Authors:
    • Xiaojuan Zhao
    • Yun Zhou
    • Yu Chen
    • Feng Yu
  • View Affiliations

  • Published online on: May 5, 2016     https://doi.org/10.3892/ol.2016.4527
  • Pages: 4245-4251
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

MicroRNAs (miRs) have been reported to be key regulators in numerous types of cancer. The aim of the present study was to investigate the role of miR‑494 in ovarian cancer. Expression of miR‑494 was analyzed in ovarian cancer tissues and cell lines by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). miR‑494 mimic or negative control was transiently transfected into A2780 and SKOV3 cell lines. A cell counting kit‑8 assay was performed to assess the effects of miR‑494 on cell proliferation, and flow cytometry was used to evaluate the apoptotic rate. The target gene of miR‑494 was detected by luciferase assay. Expression of fibroblast growth factor receptor 2 (FGFR2) was identified using RT‑qPCR and western blotting. In the present study, decreased expression of miR‑494 was observed in ovarian cancer samples and cell lines. Overexpression of miR‑494 inhibited ovarian cancer cell proliferation by inducing apoptosis. Additional investigation indicated that FGFR2 was a direct target of miR‑494. Taken together, the results of the present study suggested that miR-494 suppressed ovarian cancer cell proliferation by inducing apoptosis via targeting FGFR2.
View Figures
View References

Related Articles

Journal Cover

June-2016
Volume 11 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhao X, Zhou Y, Chen Y and Yu F: miR-494 inhibits ovarian cancer cell proliferation and promotes apoptosis by targeting FGFR2. Oncol Lett 11: 4245-4251, 2016.
APA
Zhao, X., Zhou, Y., Chen, Y., & Yu, F. (2016). miR-494 inhibits ovarian cancer cell proliferation and promotes apoptosis by targeting FGFR2. Oncology Letters, 11, 4245-4251. https://doi.org/10.3892/ol.2016.4527
MLA
Zhao, X., Zhou, Y., Chen, Y., Yu, F."miR-494 inhibits ovarian cancer cell proliferation and promotes apoptosis by targeting FGFR2". Oncology Letters 11.6 (2016): 4245-4251.
Chicago
Zhao, X., Zhou, Y., Chen, Y., Yu, F."miR-494 inhibits ovarian cancer cell proliferation and promotes apoptosis by targeting FGFR2". Oncology Letters 11, no. 6 (2016): 4245-4251. https://doi.org/10.3892/ol.2016.4527