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Identification of disease-associated pathways in pancreatic cancer by integrating genome-wide association study and gene expression data

  • Authors:
    • Jin Long
    • Zhe Liu
    • Xingda Wu
    • Yuanhong Xu
    • Chunlin Ge

  • View Affiliations

  • Published online on: May 26, 2016     https://doi.org/10.3892/ol.2016.4637
  • Pages: 537-543

  • Copyright: © Long et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

In order to additionally understand the pathogenesis of pancreatic cancer (PC), the present study conducted pathway analysis based on genome‑wide association study (GWAS) and gene expression data to predict genes that are associated with PC. GWAS data (accession no., pha002874.1) were downloaded from National Center for Biotechnology Information (NCBI) database of Genotypes and Phenotypes, which included data concerning 1,896 patients with PC and 1,939 control individuals. Gene expression data [accession no., GSE23952; human pancreatic carcinoma Panc‑1 transforming growth factor‑β (TGF‑β) treatment assay] were downloaded from NCBI Gene Expression Omnibus. Gene set enrichment analysis was used to identify significant pathways in the GWAS or gene expression profiles. Meta‑analysis was performed based on pathway analysis of the two data sources. In total, 58 and 280 pathways were identified to be significant in the GWAS and gene expression data, respectively, with 7 pathways significant in both the data profiles. Hsa 04350 TGF-β signaling pathway had the smallest meta P‑value. Other significant pathways in the two data sources were negative regulation of DNA‑dependent transcription, the nucleolus, negative regulation of RNA metabolic process, the cellular defense response, exocytosis and galactosyltransferase activity. By constructing the gene‑pathway network, 5 pathways were closely associated, apart from exocytosis and galactosyltransferase activity pathways. Among the 7 pathways, 11 key genes (2.9% out of a total of 380 genes) from the GWAS data and 43 genes (10.5% out of a total of 409 genes) from the gene expression data were differentially expressed. Only Abelson murine leukemia viral oncogene homolog 1 from the nucleolus pathway was significantly expressed in by both data sources. Overall, the results of the present analysis provide possible factors for the occurrence of PC, and the identification of the pathways and genes associated with PC provides valuable data for investigating the pathogenesis of PC in future studies.
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July-2016
Volume 12 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Long J, Liu Z, Wu X, Xu Y and Ge C: Identification of disease-associated pathways in pancreatic cancer by integrating genome-wide association study and gene expression data. Oncol Lett 12: 537-543, 2016
APA
Long, J., Liu, Z., Wu, X., Xu, Y., & Ge, C. (2016). Identification of disease-associated pathways in pancreatic cancer by integrating genome-wide association study and gene expression data. Oncology Letters, 12, 537-543. https://doi.org/10.3892/ol.2016.4637
MLA
Long, J., Liu, Z., Wu, X., Xu, Y., Ge, C."Identification of disease-associated pathways in pancreatic cancer by integrating genome-wide association study and gene expression data". Oncology Letters 12.1 (2016): 537-543.
Chicago
Long, J., Liu, Z., Wu, X., Xu, Y., Ge, C."Identification of disease-associated pathways in pancreatic cancer by integrating genome-wide association study and gene expression data". Oncology Letters 12, no. 1 (2016): 537-543. https://doi.org/10.3892/ol.2016.4637