Open Access

Long noncoding RNA are aberrantly expressed in human papillary thyroid carcinoma

  • Authors:
    • Meiliu Yang
    • Jinli Tian
    • Xin Guo
    • Ying Yang
    • Ruhua Guan
    • Mingyue Qiu
    • Yukai Li
    • Xueling Sun
    • Yanfeng Zhen
    • Yazhong Zhang
    • Chunyou Chen
    • Yanbing Li
    • Hui Fang
  • View Affiliations

  • Published online on: June 1, 2016     https://doi.org/10.3892/ol.2016.4653
  • Pages: 544-552
  • Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Long noncoding RNAs (lncRNAs) have emerged as key regulatory molecules at almost every level of gene expression regulation. The altered expression of lncRNAs is a characteristic of numerous types of cancer, and lncRNAs have been demonstrated to promote the development, invasion and metastasis of tumors through various mechanisms. However, the role of lncRNAs in papillary thyroid carcinoma (PTC) remain unclear. In the present study, differentially expressed lncRNAs and mRNAs were detected by human lncRNA microarray in three pairs of PTC and adjacent noncancerous samples. The microarray results revealed that 675 lncRNAs and 751 mRNAs were abnormally expressed in the three PTC samples compared with adjacent noncancerous samples (fold change ≥2.0; P<0.05). To validate the microarray results, 8 differentially expressed lncRNAs were randomly selected for quantitative polymerase chain reaction (qPCR). The results of qPCR were consistent with the microarray data; the 8 lncRNAs had an aberrant expression in the PTC samples compared with the adjacent noncancerous samples. Gene ontology and pathway analysis indicated that there were 7 downregulated pathways and 29 upregulated pathways in PTC. LncRNA classification and subgroup analysis revealed 7 pairs of enhancer‑like lncRNA‑mRNA, 9 pairs of antisense lncRNA‑mRNA and 45 pairs of lncRNA‑mRNA were differentially expressed between PTC and their paired noncancerous samples. In conclusion, the present study identified a series of novel PTC‑associated lncRNAs. Further study with these lncRNAs is instrumental for the identification of novel target molecules that could lead to improved diagnosis and treatment for PTC.
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July-2016
Volume 12 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Yang M, Tian J, Guo X, Yang Y, Guan R, Qiu M, Li Y, Sun X, Zhen Y, Zhang Y, Zhang Y, et al: Long noncoding RNA are aberrantly expressed in human papillary thyroid carcinoma. Oncol Lett 12: 544-552, 2016
APA
Yang, M., Tian, J., Guo, X., Yang, Y., Guan, R., Qiu, M. ... Fang, H. (2016). Long noncoding RNA are aberrantly expressed in human papillary thyroid carcinoma. Oncology Letters, 12, 544-552. https://doi.org/10.3892/ol.2016.4653
MLA
Yang, M., Tian, J., Guo, X., Yang, Y., Guan, R., Qiu, M., Li, Y., Sun, X., Zhen, Y., Zhang, Y., Chen, C., Li, Y., Fang, H."Long noncoding RNA are aberrantly expressed in human papillary thyroid carcinoma". Oncology Letters 12.1 (2016): 544-552.
Chicago
Yang, M., Tian, J., Guo, X., Yang, Y., Guan, R., Qiu, M., Li, Y., Sun, X., Zhen, Y., Zhang, Y., Chen, C., Li, Y., Fang, H."Long noncoding RNA are aberrantly expressed in human papillary thyroid carcinoma". Oncology Letters 12, no. 1 (2016): 544-552. https://doi.org/10.3892/ol.2016.4653