Hypermethylation reduces the expression of PNPLA7 in hepatocellular carcinoma

Zhang,Xiaojiao; Zhang,Jun; Wang,Rui; Guo,Shicheng; Zhang,Huilu; Ma,Yanyun; Liu,Qingmei; Chu,Haiyan; Xu,Xianghong; Zhang,Yitong; Yang,Dongqin; Wang,Jiucun; Liu,Jie

doi:10.3892/ol.2016.4660

Hypermethylation reduces the expression of PNPLA7 in hepatocellular carcinoma

Authors:
- Xiaojiao Zhang
- Jun Zhang
- Rui Wang
- Shicheng Guo
- Huilu Zhang
- Yanyun Ma
- Qingmei Liu
- Haiyan Chu
- Xianghong Xu
- Yitong Zhang
- Dongqin Yang
- Jiucun Wang
- Jie Liu
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Affiliations: Department of Digestive Diseases, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China, Ministry of Education (MOE) Key Laboratory of Contemporary Anthropology, State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200433, P.R. China
Published online on: June 1, 2016 https://doi.org/10.3892/ol.2016.4660
Pages: 670-674

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Abstract

Liver cancer has a high morbidity and mortality rate, and is one of the most common types of cancer in men. PNPLA7 is a member of the patatin‑like phospholipase domain‑containing protein family which is involved in triglyceride hydrolysis, energy metabolism and lipid droplet metabolism. The liver is the most important energy metabolism organ; whether PNPLA7 is deregulated in liver cancer has not been previously reported. In the present study, reverse transcription‑quantitative polymerase chain reaction and subsequent methylation analysis provided evidence that PNPLA7 is down‑regulated in hepatocellular carcinoma (HCC) cell lines and tissue samples, via the mechanism of transcriptional silencing by promoter hypermethylation. These results may provide novel insights for HCC diagnosis.

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