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Article Open Access

Differential expression of mucins in Middle Eastern patients with colorectal cancer

  • Authors:
    • Khayal Al‑Khayal
    • Maha Abdulla
    • Omar Al‑Obaid
    • Ahmad Zubaidi
    • Mansoor‑Ali Vaali‑Mohammed
    • Abdulmalik Alsheikh
    • Rehan Ahmad
  • View Affiliations / Copyright

    Affiliations: Colorectal Research Centre, Department of Surgery, King Khalid University Hospital, College of Medicine, King Saud University, Riyadh 11472, Kingdom of Saudi Arabia, Department of Pathology, King Khalid University Hospital, College of Medicine, King Saud University, Riyadh 11472, Kingdom of Saudi Arabia
    Copyright: © Al‑Khayal et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 393-400
    |
    Published online on: June 1, 2016
       https://doi.org/10.3892/ol.2016.4672
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Abstract

Mucin overexpression has been implicated in the tumorigenesis and progression of colorectal carcinoma (CRC). However, data obtained on the prognostic importance of mucin expression in CRC is inconsistent. Due to lack of data on mucin expression and the increase in CRC incidence in Saudi Arabia, the aim of the present study was to analyze the mucin expression profile in patients with CRC in this ethnic group. The present study consisted of 22 patients that underwent surgery for CRC. Histopathological and immunohistochemical staining was performed on CRC tumor and adjacent normal tissues. A tissue microarray was prepared from the tumor and normal adjacent samples to investigate the mucin expression profile using immunohistochemistry. Formalin‑fixed paraffin‑embedded human colorectal cancer tissues were immunostained with mucin 1 (MUC1), mucin 2 (MUC2) and mucin 5AC (MUC5AC) antibodies. Associations between mucin expression and histopathological variables were evaluated. The present study indicated that MUC1 was highly expressed in early (stage I and II; P=0.0016) and late (stage III and IV; P<0.0001) stage CRC tissues compared to normal adjacent tissues. However, MUC2 expression was observed to be downregulated in early and late stage CRC tissues compared to normal and adjacent tissues. Furthermore, serum MUC1 levels were observed to be increased in early and late stage CRC. The present findings indicate that MUC1 expression was significantly higher in early and late stage CRC tissues and MUC2 was downregulated in CRC tissues compared with normal adjacent tissues, and serum MUC1 protein was significantly higher in CRC patients compared to control serum. In conclusion, during colorectal tumorigenesis the pattern of MUC1 and MUC2 expression is altered in Saudi Arabian patients with CRC compared with normal. A higher expression of MUC1 may be used as an independent biomarker in various stages of CRC tumors, which would aid in the early detection of CRC.
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1 

Kufe DW: Mucins in cancer: Function, prognosis and therapy. Nat Rev Cancer. 9:874–885. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Kufe DW: MUC1-C oncoprotein as a target in breast cancer: Activation of signaling pathway and therapeutic approaches. Oncogene. 32:1073–1081. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Macao B, Johansson DG, Hansson GC and Härd T: Autoproteolysis coupled to protein folding in the SEA domain of the membrane-bound MUC1 mucin. Nat Struct Mol Biol. 13:71–76. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Siddiqui J, Abe M, Hayes D, Shani E, Yunis E and Kufe D: Isolation and sequencing of a cDNA coding for the human DF3 breast carcinoma-associated antigen. Proc Natl Acad Sci USA. 85:2320–2323. 1988. View Article : Google Scholar : PubMed/NCBI

5 

Karin M and Greten FR: NF-kappaB: Linking inflammation and immunity to cancer development and progression. Nat Rev Immunol. 10:749–759. 2005. View Article : Google Scholar

6 

Ahmad R, Raina D, Trivedi V, Ren J, Rajabi H, Kharbanda S and Kufe D: MUC1 oncoprotein activates the I kappaB kinase complex and constitutive NF-kappaB signaling. Nat Cell Biol. 9:1419–1427. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Ahmad R, Raina D, Joshi MD, Kawano T, Ren J, Kharbanda S and Kufe D: MUC1-C oncoprotein functions as a direct activator of the nuclear factor-kappaB p65 transcription factor. Cancer Res. 69:7013–7021. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Vinall LE, King M, Novelli M, Green CA, Daniels G, Hilkens J, Sarner M and Swallow DM: Altered expression and allelic association of the hypervariable membrane mucin MUC1 in Helicobacter pylori gastritis. Gastroenterology. 123:41–49. 2002. View Article : Google Scholar : PubMed/NCBI

9 

Huang L, Chen D, Liu D, Yin L, Kharbanda S and Kufe D: MUC1 oncoprotein blocks glycogen synthase kinase 3 beta-mediated phosphorylation and degradation of beta-catenin. Cancer Res. 65:10413–10422. 2005. View Article : Google Scholar : PubMed/NCBI

10 

Kanoh A, Takeuchi H, Kato K, Waki M, Usami K and Irimura T: Interleukin-4 induces specific pp-GalNAc-T expression and alterations in mucin O-glycosylation in colonic epithelial cells. Biochim Biophys Acta. 1780:577–584. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Byrd JC and Bresalier RS: Mucins and mucin binding proteins in colorectal cancer. Cancer Metastasis Rev. 23:77–99. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Johansson ME, Phillipson M, Petersson J, Velcich A, Holm L and Hansson GC: The inner of the two Muc2 mucin-dependent mucus layers in colon is devoid of bacteria. Proc Natl Acad Sci USA. 105:15064–15069. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Xavier RJ and Podolsky DK: Unravelling the pathogenesis of inflammatory bowel disease. Nature. 448:427–434. 2007. View Article : Google Scholar : PubMed/NCBI

14 

Feagins LA, Souza RF and Spechler SJ: Carcinogenesis in IBD: Potential targets for the prevention of colorectal cancer. Nat Rev Gastroenterol Hepatol. 6:297–305. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Hollingsworth MA and Swanson BJ: Mucins in cancer: Protection and control of the cell surface. Nat Rev Cancer. 4:45–60. 2004. View Article : Google Scholar : PubMed/NCBI

16 

Vincent A, Perrais M, Desseyn JL, Aubert JP, Pigny P and Van Seuningen I: Epigenetic regulation (DNA methylation, histone modifications) of the 11p15 mucin genes (MUC2, MUC5AC, MUC5B, MUC6) in epithelial cancer cells. Oncogene. 26:6566–6576. 2007. View Article : Google Scholar : PubMed/NCBI

17 

Bu XD, Li N, Tian XQ, Li L, Wang JS, Yu XJ and Huang PL: Altered expression of MUC2 and MUC5AC in progression of colorectal carcinoma. World J Gastroenterol. 16:4089–4094. 2010. View Article : Google Scholar : PubMed/NCBI

18 

López-Ferrer A, Curull V, Barranco C, Garrido M, Lloreta J, Real FX and de Bolós C: Mucins as differentiation markers in bronchial epithelium. Squamous cell carcinoma and adenocarcinoma display similar expression patterns. Am J Respir Cell Mol Biol. 24:22–29. 2001. View Article : Google Scholar : PubMed/NCBI

19 

Yu CJ, Shih JY, Lee YC, Shun CT, Yuan A and Yang PC: Sialyl Lewis antigens: Association with MUC5AC protein and correlation with post-operative recurrence of non-small cell lung cancer. Lung Cancer. 47:59–67. 2005. View Article : Google Scholar : PubMed/NCBI

20 

Duncan TJ, Watson NF, Al-Attari AH, Scholefield JH and Durrant LG: The role of MUC1 and MUC3 in the biology and prognosis of colorectal cancer. World J Sur Oncol. 5:312007. View Article : Google Scholar

21 

Kocer B, Soran A, Erdogan S, Karabeyoglu M, Yildirim O, Eroglu A, Bozkurt B and Cengiz O: Expression of MUC5AC in colorectal carcinoma and relationship with prognosis. Pathol Int. 52:470–477. 2002. View Article : Google Scholar : PubMed/NCBI

22 

Mosli MH and Al-Ahwal MS: Colorectal cancer in the kingdom of Saudi Arabia: Need for screening. Asian Pac J Cancer Prev. 13:3809–3813. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Gado A, Ebeid B, Abdelmohsen A and Axon A: Colorectal cancer in Egypt is commoner in young people: Is this cause for alarm. Alexandria J Med. 50:197–201. 2014. View Article : Google Scholar

24 

Greene FL: TNM staging for malignancies of the digestive tract: 2003 changes and beyond. Semin Surg Oncol. 21:23–29. 2003. View Article : Google Scholar : PubMed/NCBI

25 

Sobin LH and Wittekind C: TNM Classification of Malignant Tumours (6th). John Wiley & Sons. Hoboken, NJ: 2002.

26 

Hamilton SR and Aaltonen L: Tumours of small intestine. In: World Health Organization Classification of Tumours. Pathology and Genetics of Tumours of the Digestive System. IARC Press. (Lyon). 69–91. 2000.

27 

Rimm DL, Camp RL, Charette LA, Costa J, Olsen DA and Reiss M: Tissue microarray: A new technology for amplification of tissue resources. Cancer J. 7:24–31. 2001.PubMed/NCBI

28 

Biemer-Hüttmann AE, Walsh MD, McGuckin MA, et al: Mucin core protein expression in colorectal cancers with high levels of microsatellite instability indicates a novel pathway of morphogenesis. Clin Cancer Res. 6:1909–1916. 2000.PubMed/NCBI

29 

Farhat MH, Barada KA, Tawil AN, Itani DM, Hatoum HA and Shamseddine AI: Effect of mucin production on survival in colorectal cancer: A case-control study. World J Gastroenterol. 14:6981–6985. 2008. View Article : Google Scholar : PubMed/NCBI

30 

Baldus SE, Mönig SP, Hanisch FG, Zirbes TK, Flucke U, Oelert S, Zilkens G, Madejczik B, Thiele J, Schneider PM, et al: Comparative evaluation of the prognostic value of MUC1, MUC2, sialyl-Lewis(a) and sialyl-Lewis(x) antigens in colorectal adenocarcinoma. Histopathology. 40:440–449. 2002. View Article : Google Scholar : PubMed/NCBI

31 

Voloshanenko O, Erdmann G, Dubash TD, Augustin I, Metzig M, Moffa G, Hundsrucker C, Kerr G, Sandmann T, Anchang B, et al: Wnt secretion is required to maintain high levels of Wnt activity in colon cancer cells. Nat Commun. 4:26102013. View Article : Google Scholar : PubMed/NCBI

32 

Ajioka Y, Allison LJ and Jass JR: Significance of MUC1 and MUC2 mucin expression in colorectal cancer. J Clin Pathol. 49:560–564. 1996. View Article : Google Scholar : PubMed/NCBI

33 

Gratchev A, Siedow A, Bumke-Vogt C, Hummel M, Foss HD, Hanski ML, Kobalz U, Mann B, Lammert H, Mansmann U, et al: Regulation of the intestinal mucin MUC2 gene expression in vivo: Evidence for the role of promoter methylation. Cancer Lett. 168:71–80. 2001. View Article : Google Scholar : PubMed/NCBI

34 

Ookawa K, Kudo T, Aizawa S, Saito H and Tsuchida S: Transcriptional activation of the MUC2 gene by p53. J Biol Chem. 277:48270–48275. 2002. View Article : Google Scholar : PubMed/NCBI

35 

Biemer-Hüttmann AE, Walsh MD, McGuckin MA, Simms LA, Young J, Leggett BA and Jass JR: Mucin core protein expression in colorectal cancers with high levels of microsatellite instability indicates a novel pathway of morphogenesis. Clin Cancer Res. 6:1909–1916. 2000.PubMed/NCBI

36 

Lugli A, Zlobec I, Baker K, Minoo P, Tornillo L, Terracciano L and Jass JR: Prognostic significance of mucins in colorectal cancer with different DNA mismatch-repair status. J Clin Pathol. 60:534–539. 2007. View Article : Google Scholar : PubMed/NCBI

37 

Rabassa ME, Croce M, Pereyra A and Segal-Eiras A: MUC1 expression and anti-MUC1 serum immune response in head and neck squamous cell carcinoma (HNSCC): A multivariate analysis. BMC Cancer. 6:2532006. View Article : Google Scholar : PubMed/NCBI

38 

Ahnen DJ, Wade SW, Jones WF, Sifri R, Silveiras Mendoza J, Greenamyer J, Guiffre S, Axilbund J, Spiegel A and You YN: The increasing incidence of young onset colorectal cancer: A call to action. Mayo Clinic Proc. 89:216–224. 2014. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Al‑Khayal K, Abdulla M, Al‑Obaid O, Zubaidi A, Vaali‑Mohammed MA, Alsheikh A and Ahmad R: Differential expression of mucins in Middle Eastern patients with colorectal cancer. Oncol Lett 12: 393-400, 2016.
APA
Al‑Khayal, K., Abdulla, M., Al‑Obaid, O., Zubaidi, A., Vaali‑Mohammed, M., Alsheikh, A., & Ahmad, R. (2016). Differential expression of mucins in Middle Eastern patients with colorectal cancer. Oncology Letters, 12, 393-400. https://doi.org/10.3892/ol.2016.4672
MLA
Al‑Khayal, K., Abdulla, M., Al‑Obaid, O., Zubaidi, A., Vaali‑Mohammed, M., Alsheikh, A., Ahmad, R."Differential expression of mucins in Middle Eastern patients with colorectal cancer". Oncology Letters 12.1 (2016): 393-400.
Chicago
Al‑Khayal, K., Abdulla, M., Al‑Obaid, O., Zubaidi, A., Vaali‑Mohammed, M., Alsheikh, A., Ahmad, R."Differential expression of mucins in Middle Eastern patients with colorectal cancer". Oncology Letters 12, no. 1 (2016): 393-400. https://doi.org/10.3892/ol.2016.4672
Copy and paste a formatted citation
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Spandidos Publications style
Al‑Khayal K, Abdulla M, Al‑Obaid O, Zubaidi A, Vaali‑Mohammed MA, Alsheikh A and Ahmad R: Differential expression of mucins in Middle Eastern patients with colorectal cancer. Oncol Lett 12: 393-400, 2016.
APA
Al‑Khayal, K., Abdulla, M., Al‑Obaid, O., Zubaidi, A., Vaali‑Mohammed, M., Alsheikh, A., & Ahmad, R. (2016). Differential expression of mucins in Middle Eastern patients with colorectal cancer. Oncology Letters, 12, 393-400. https://doi.org/10.3892/ol.2016.4672
MLA
Al‑Khayal, K., Abdulla, M., Al‑Obaid, O., Zubaidi, A., Vaali‑Mohammed, M., Alsheikh, A., Ahmad, R."Differential expression of mucins in Middle Eastern patients with colorectal cancer". Oncology Letters 12.1 (2016): 393-400.
Chicago
Al‑Khayal, K., Abdulla, M., Al‑Obaid, O., Zubaidi, A., Vaali‑Mohammed, M., Alsheikh, A., Ahmad, R."Differential expression of mucins in Middle Eastern patients with colorectal cancer". Oncology Letters 12, no. 1 (2016): 393-400. https://doi.org/10.3892/ol.2016.4672
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