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Article

Proliferation and metastatic potential of endometrial cancer cells in response to metformin treatment in a high versus normal glucose environment

  • Authors:
    • Amanda de Barros Machado
    • Vania dos Reis
    • Sebastian Weber
    • Julia Jauckus
    • Ilma Simoni Brum
    • Helena von Eye Corleta
    • Thomas Strowitzki
    • Edison Capp
    • Ariane Germeyer
  • View Affiliations / Copyright

    Affiliations: Laboratory of Molecular Endocrine Biology and Tumor Biology, Department of Physiology, Institute of Basic Sciences of Health, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul 90050-170, Brazil, Department of Gynecological Endocrinology and Reproductive Medicine, University Hospital Heidelberg, D‑69120 Heidelberg, Germany
  • Pages: 3626-3632
    |
    Published online on: August 23, 2016
       https://doi.org/10.3892/ol.2016.5041
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Abstract

In order to improve our understanding of the potential preventive and therapeutic role of metformin, the present study aimed to investigate the capability of low‑dose metformin in the efficient inhibition of cancer development and the reduction of the metastasis of endometrial adenocarcinoma type I and primary endometrial epithelial cells (eEPs), with the drug acting as a treatment in a hyperinsulinemic environment exposed to high and normal glucose conditions. The Ishikawa endometrial adenocarcinoma cell line and primary eEPs were exposed to an environment with high (17 mM) or normal glucose (5 mM) and treated with insulin, low‑dose metformin (0.1 mM) or a combined treatment. Metastatic potential was assessed by migration and invasion assays, and relative cell proliferation was determined. Metformin at a low dose potently inhibited the insulin action, decreasing the ability of the endometrial cancer (EC) cell line to migrate and invade in a high and normal glucose environment, and decreasing the migration ability of the primary eEPs. In the EC cell line, the insulin treatment increased the proliferation, without any subsequent reduction of proliferation by the addition of 0.1 mM metformin; however, relative cell proliferation sensitivity to metformin was observed in the range between 1 and 5 mM regardless of the glucose concentration present. Overall, metformin at 0.1 mM is not efficient enough to decrease the proliferation in an EC cell line. However, at this concentration, metformin can inhibit the insulin action in endometrial epithelial cancer cells, demonstrating an anti‑metastatic effect in high and normal glucose environments.
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Copy and paste a formatted citation
Spandidos Publications style
de Barros Machado A, dos Reis V, Weber S, Jauckus J, Brum IS, von Eye Corleta H, Strowitzki T, Capp E and Germeyer A: Proliferation and metastatic potential of endometrial cancer cells in response to metformin treatment in a high versus normal glucose environment. Oncol Lett 12: 3626-3632, 2016.
APA
de Barros Machado, A., dos Reis, V., Weber, S., Jauckus, J., Brum, I.S., von Eye Corleta, H. ... Germeyer, A. (2016). Proliferation and metastatic potential of endometrial cancer cells in response to metformin treatment in a high versus normal glucose environment. Oncology Letters, 12, 3626-3632. https://doi.org/10.3892/ol.2016.5041
MLA
de Barros Machado, A., dos Reis, V., Weber, S., Jauckus, J., Brum, I. S., von Eye Corleta, H., Strowitzki, T., Capp, E., Germeyer, A."Proliferation and metastatic potential of endometrial cancer cells in response to metformin treatment in a high versus normal glucose environment". Oncology Letters 12.5 (2016): 3626-3632.
Chicago
de Barros Machado, A., dos Reis, V., Weber, S., Jauckus, J., Brum, I. S., von Eye Corleta, H., Strowitzki, T., Capp, E., Germeyer, A."Proliferation and metastatic potential of endometrial cancer cells in response to metformin treatment in a high versus normal glucose environment". Oncology Letters 12, no. 5 (2016): 3626-3632. https://doi.org/10.3892/ol.2016.5041
Copy and paste a formatted citation
x
Spandidos Publications style
de Barros Machado A, dos Reis V, Weber S, Jauckus J, Brum IS, von Eye Corleta H, Strowitzki T, Capp E and Germeyer A: Proliferation and metastatic potential of endometrial cancer cells in response to metformin treatment in a high versus normal glucose environment. Oncol Lett 12: 3626-3632, 2016.
APA
de Barros Machado, A., dos Reis, V., Weber, S., Jauckus, J., Brum, I.S., von Eye Corleta, H. ... Germeyer, A. (2016). Proliferation and metastatic potential of endometrial cancer cells in response to metformin treatment in a high versus normal glucose environment. Oncology Letters, 12, 3626-3632. https://doi.org/10.3892/ol.2016.5041
MLA
de Barros Machado, A., dos Reis, V., Weber, S., Jauckus, J., Brum, I. S., von Eye Corleta, H., Strowitzki, T., Capp, E., Germeyer, A."Proliferation and metastatic potential of endometrial cancer cells in response to metformin treatment in a high versus normal glucose environment". Oncology Letters 12.5 (2016): 3626-3632.
Chicago
de Barros Machado, A., dos Reis, V., Weber, S., Jauckus, J., Brum, I. S., von Eye Corleta, H., Strowitzki, T., Capp, E., Germeyer, A."Proliferation and metastatic potential of endometrial cancer cells in response to metformin treatment in a high versus normal glucose environment". Oncology Letters 12, no. 5 (2016): 3626-3632. https://doi.org/10.3892/ol.2016.5041
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