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Article Open Access

Predictive value of microparticle-associated tissue factor activity for permeability glycoprotein-mediated multidrug resistance in cancer

  • Authors:
    • Antonio Angelini
    • Sebastiano Miscia
    • Maria Antonietta Centurione
    • Roberta Di Pietro
    • Lucia Centurione
  • View Affiliations / Copyright

    Affiliations: Department of Medicine and Aging Science, School of Medicine and Health Science, ‘G. d'Annunzio’ University of Chieti‑Pescara, I‑66013 Chieti, Italy, Institute of Molecular Genetics, National Research Council ‑ Pavia, Chieti Unit, I‑66013 Chieti, Italy
    Copyright: © Angelini et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3273-3277
    |
    Published online on: September 8, 2016
       https://doi.org/10.3892/ol.2016.5105
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Abstract

Multidrug resistance (MDR) protein 1, which is also known as permeability glycoprotein (Pgp), and tissue factor (TF) are recurrently overexpressed on the surface of cancer cells, likely in response to stimuli such as chemotherapy. Microparticles (MPs) released from cancer cells into the bloodstream express tumour markers on their surface that may be useful as predictive biomarkers for evaluating disease progression. The present study measured the level of TF/factor VII (FVII)‑dependent coagulation of MPs isolated from the plasma of cancer patients with various tumours, who were undergoing chemotherapy. Furthermore, Pgp expression on the surface of MPs was evaluated by immunohistochemistry. A total of 50 cancer patients, as well as 10 healthy volunteers, were enrolled in the present study. MP‑associated TF/FVII‑dependent coagulation pathways were evaluated as the effect of an anti‑FVII antibody on the time to thrombin generation, as compared with controls treated with saline. The significantly lengthened times of coagulation [obtained in 20/50 samples (36.5 ± 16%) after treatment with anti‑FVIIa when compared with controls] suggest the presence of TF activity is associated with circulating MPs. Furthermore, the 20 MP/TF‑positive samples were associated with Pgp overexpression on their surface. Conversely, in the remaining samples (n=30), treatment with the anti‑FVIIa antibody did not significantly lengthen the time to clotting (<10%), and Pgp overexpression was not detected. In addition, in the control samples from healthy individuals, Pgp expression at the plasma membrane and clotting in the presence of the anti‑FVII antibody were not observed, indicating the absence of MPs. The present study demonstrated that MPs in the blood of cancer patients promoted fibrin generation via TF/FVII‑dependent pathways, thus suggesting that the evaluation of MP‑TF activity may have a predictive value for Pgp‑mediated MDR in various cancer types. Although further studies are required, the measurement of plasma MP‑associated TF activity as a predictive biomarker may provide novel therapeutic perspectives to improve the prognosis and effectiveness of anti‑cancer drugs in patients who are at a high-risk of Pgp-mediated MDR.
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Copy and paste a formatted citation
Spandidos Publications style
Angelini A, Miscia S, Centurione MA, Di Pietro R and Centurione L: Predictive value of microparticle-associated tissue factor activity for permeability glycoprotein-mediated multidrug resistance in cancer. Oncol Lett 12: 3273-3277, 2016.
APA
Angelini, A., Miscia, S., Centurione, M.A., Di Pietro, R., & Centurione, L. (2016). Predictive value of microparticle-associated tissue factor activity for permeability glycoprotein-mediated multidrug resistance in cancer. Oncology Letters, 12, 3273-3277. https://doi.org/10.3892/ol.2016.5105
MLA
Angelini, A., Miscia, S., Centurione, M. A., Di Pietro, R., Centurione, L."Predictive value of microparticle-associated tissue factor activity for permeability glycoprotein-mediated multidrug resistance in cancer". Oncology Letters 12.5 (2016): 3273-3277.
Chicago
Angelini, A., Miscia, S., Centurione, M. A., Di Pietro, R., Centurione, L."Predictive value of microparticle-associated tissue factor activity for permeability glycoprotein-mediated multidrug resistance in cancer". Oncology Letters 12, no. 5 (2016): 3273-3277. https://doi.org/10.3892/ol.2016.5105
Copy and paste a formatted citation
x
Spandidos Publications style
Angelini A, Miscia S, Centurione MA, Di Pietro R and Centurione L: Predictive value of microparticle-associated tissue factor activity for permeability glycoprotein-mediated multidrug resistance in cancer. Oncol Lett 12: 3273-3277, 2016.
APA
Angelini, A., Miscia, S., Centurione, M.A., Di Pietro, R., & Centurione, L. (2016). Predictive value of microparticle-associated tissue factor activity for permeability glycoprotein-mediated multidrug resistance in cancer. Oncology Letters, 12, 3273-3277. https://doi.org/10.3892/ol.2016.5105
MLA
Angelini, A., Miscia, S., Centurione, M. A., Di Pietro, R., Centurione, L."Predictive value of microparticle-associated tissue factor activity for permeability glycoprotein-mediated multidrug resistance in cancer". Oncology Letters 12.5 (2016): 3273-3277.
Chicago
Angelini, A., Miscia, S., Centurione, M. A., Di Pietro, R., Centurione, L."Predictive value of microparticle-associated tissue factor activity for permeability glycoprotein-mediated multidrug resistance in cancer". Oncology Letters 12, no. 5 (2016): 3273-3277. https://doi.org/10.3892/ol.2016.5105
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