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Methylation of promoter region of CDX2 gene in colorectal cancer

  • Authors:
    • Yunshuai Wang
    • Zhaohui Li
    • Wenxian Li
    • Shuaifeng Liu
    • Baowei Han
  • View Affiliations / Copyright

    Affiliations: Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang, Henan 471000, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3229-3233
    |
    Published online on: September 9, 2016
       https://doi.org/10.3892/ol.2016.5109
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Abstract

The incidence of colorectal cancer is on the increase owing to changes in daily diet. In the present study, the methylation status of caudal type homeobox transcription factor 2 (CDX2) gene in lesion tissue of colorectal cancer (CRC) was investigated. Additionally, the correlation between the promoter methylation of CDX2 gene, CRC and gene expression in patients with CRC and normal population was examined. Between April 2014 and May 2015 78 cases with CRC were enrolled in the study. Using methylation-specific polymerase chain reaction (PCR), the promoter methylation of CDX2 in normal tissues and colorectal tissues was examinned. Through the fluorescence quantitative PCR technique, the expression levels of CDX2 gene were determined in a normal population and lesion tissue of patients with CRC. At the same time, we evaluated the levels of the CDX2 gene product in the normal population and lesion tissue of patients with CRC. The results showed that the methylation rate of the promoter region of CDX2 gene in normal colorectal tissue was 43.5%, whereas that in the lesion tissue of CRC was 78.5%. The result was statistically significant (P<0.05). The quantity of mRNA and protein expression of CDX2 gene in colorectal and normal tissue was significantly different (P<0.05). In conclusion, the methylation of the CDX2 gene promoter region was associated with risk of CRC, i.e., methylation of the promoter region of CDX2 gene favors the occurrence of CRC.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Y, Li Z, Li W, Liu S and Han B: Methylation of promoter region of CDX2 gene in colorectal cancer. Oncol Lett 12: 3229-3233, 2016.
APA
Wang, Y., Li, Z., Li, W., Liu, S., & Han, B. (2016). Methylation of promoter region of CDX2 gene in colorectal cancer. Oncology Letters, 12, 3229-3233. https://doi.org/10.3892/ol.2016.5109
MLA
Wang, Y., Li, Z., Li, W., Liu, S., Han, B."Methylation of promoter region of CDX2 gene in colorectal cancer". Oncology Letters 12.5 (2016): 3229-3233.
Chicago
Wang, Y., Li, Z., Li, W., Liu, S., Han, B."Methylation of promoter region of CDX2 gene in colorectal cancer". Oncology Letters 12, no. 5 (2016): 3229-3233. https://doi.org/10.3892/ol.2016.5109
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Li Z, Li W, Liu S and Han B: Methylation of promoter region of CDX2 gene in colorectal cancer. Oncol Lett 12: 3229-3233, 2016.
APA
Wang, Y., Li, Z., Li, W., Liu, S., & Han, B. (2016). Methylation of promoter region of CDX2 gene in colorectal cancer. Oncology Letters, 12, 3229-3233. https://doi.org/10.3892/ol.2016.5109
MLA
Wang, Y., Li, Z., Li, W., Liu, S., Han, B."Methylation of promoter region of CDX2 gene in colorectal cancer". Oncology Letters 12.5 (2016): 3229-3233.
Chicago
Wang, Y., Li, Z., Li, W., Liu, S., Han, B."Methylation of promoter region of CDX2 gene in colorectal cancer". Oncology Letters 12, no. 5 (2016): 3229-3233. https://doi.org/10.3892/ol.2016.5109
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