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Therapeutic targeting of liver cancer with a recombinant DNA vaccine containing the hemagglutinin-neuraminidase gene of Newcastle disease virus via apoptotic-dependent pathways

  • Authors:
    • Li‑Gang Chen
    • Yuan‑Sheng Liu
    • Tang‑Hui Zheng
    • Xu Chen
    • Ping Li
    • Chuan‑Xing Xiao
    • Jian‑Lin Ren
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Zhongshan Hospital Affiliated to Xiamen University, Xiamen, Fujian 361004, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3344-3350
    |
    Published online on: September 9, 2016
       https://doi.org/10.3892/ol.2016.5114
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Abstract

A total of ~38.6 million mortalities occur due to liver cancer annually, worldwide. Although a variety of therapeutic methods are available, the efficacy of treatment at present is extremely limited due to an increased risk of malignancy and inherently poor prognosis of liver cancer. Gene therapy is considered a promising option, and has shown notable potential for the comprehensive therapy of liver cancer, in keeping with advances that have been made in the development of cancer molecular biology. The present study aimed to investigate the synergistic effects of the abilities of the hemagglutinin neuraminidase protein of Newcastle disease virus (NDV), the pro‑apoptotic factor apoptin from chicken anaemia virus, and the interferon‑γ inducer interleukin‑18 (IL‑18) in antagonizing liver cancer. Therefore, a recombinant DNA plasmid expressing the three exogenous genes, VP3, IL‑18 and hemagglutinin neuraminidase (HN), was constructed. Flow cytometry, acridine orange/ethidium bromide staining and analysis of caspase‑3 activity were performed in H22 cell lines transfected with the recombinant DNA plasmid. In addition, 6-week-old C57BL/6 mice were used to establish a H22 hepatoma‑bearing mouse model. Mice tumor tissue was analyzed by immunohistochemistry and scanning electron microscopy. The results of the present study revealed that the recombinant DNA vaccine containing the VP3, IL‑18 and HN genes inhibited cell proliferation and induced autophagy via the mitochondrial pathway in vivo and in vitro.
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1 

Brand K, Löser P, Arnold W, Bartels T and Strauss M: Tumor cell-specific transgene expression prevents liver toxicity of the adeno-HSVtk/GCV approach. Gene Ther. 5:1363–1371. 1998. View Article : Google Scholar : PubMed/NCBI

2 

Baqué P, Pierrefite-Carle V, Gavelli A, Brossette N, Benchimol D, Bourgeon A, Staccini P, Saint-Paul MC and Rossi B: Naked DNA injection for liver metastases treatment in rats. Hepatology. 35:1144–1152. 2002. View Article : Google Scholar : PubMed/NCBI

3 

Li Y, Li B, Li CJ and Li LJ: Key points of basic theories and clinical practice in rAd-p53 (Gendicine™) gene therapy for solid malignant tumors. Expert Opin Biol Ther. 15:437–454. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Baliaka A, Zarogoulidis P, Domvri K, Hohenforst-Schmidt W, Sakkas A, Huang H, Le Pivert P, Koliakos G, Koliakou E, Kouzi-Koliakos K, et al: Intratumoral gene therapy versus intravenous gene therapy for distant metastasis control with 2-diethylaminoethyl-dextran methyl methacrylate copolymer non-viral vector-p53. Gene Ther. 21:158–167. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Niu HX, Du T, Xu ZF, Zhang XK and Wang RG: Role of wild type p53 and double suicide genes in interventional therapy of liver cancer in rabbits. Acta Cir Bras. 27:522–528. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Fargnoli AS, Katz MG, Williams RD, Margulies KB and Bridges CR: A needleless liquid jet injection delivery method for cardiac gene therapy: A comparative evaluation versus standard routes of delivery reveals enhanced therapeutic retention and cardiac specific gene expression. J Cardiovasc Transl Res. 7:756–767. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Wang J, Wang C, Feng N, Wang H, Zheng X, Yang S, Gao Y, Xia X, Yin R, Liu X, et al: Development of a reverse genetics system based on RNA polymerase II for Newcastle disease virus genotype VII. Virus Genes. 50:152–155. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Alexander DJ: Newcastle disease and other avian paramyxoviruses. Rev Sci Tech. 19:443–462. 2000.PubMed/NCBI

9 

Zeng J, Fournier P and Schirrmacher V: Induction of interferon-alpha and tumor necrosis factor-related apoptosis-inducing ligand in human blood mononuclear cells by hemagglutinin-neuraminidase but not F protein of Newcastle disease virus. Virology. 297:19–30. 2002. View Article : Google Scholar : PubMed/NCBI

10 

Sui H, Bai Y, Wang K, Li X, Song C, Fu F, Zhang Y and Li L: The anti-tumor effect of Newcastle disease virus HN protein is influenced by differential subcellular targeting. Cancer Immunol Immunother. 59:989–999. 2010. View Article : Google Scholar : PubMed/NCBI

11 

Rajmani RS, Singh PK, Kumar G Ravi, Saxena S, Singh LV, Kumar R, Sahoo AP, Gupta SK, Chaturvedi U and Tiwari AK: In-vitro characterization and evaluation of apoptotic potential of bicistronic plasmid encoding HN gene of Newcastle disease virus and human TNF-α. Anim Biotechnol. 26:112–119. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Brenu EW, van Driel ML, Staines DR, Ashton KJ, Ramos SB, Keane J, Klimas NG and Marshall-Gradisnik SM: Immunological abnormalities as potential biomarkers in chronic fatigue syndrome/myalgic encephalomyelitis. J Transl Med. 9:812011. View Article : Google Scholar : PubMed/NCBI

13 

Dong D, Gao J, Sun Y, Long Y, Li M, Zhang D, Gong J, Xu L, Li L, Qin S, et al: Adenovirus-mediated co-expression of the TRAIL and HN genes inhibits growth and induces apoptosis in Marek's disease tumor cell line MSB-1. Cancer Cell Int. 15:202015. View Article : Google Scholar : PubMed/NCBI

14 

Guan G, Jin N, Li X, Sun L, Jin C, Lou W, Shi P and Hao Y: Anti-tumor effects on human laryngeal carcinoma Hep-2 of recombinant fowlpox virus expressing chicken anemia virus Apoptin gene. Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 23:264–266. 2009.(In Chinese). PubMed/NCBI

15 

Natesan S, Kataria JM, Dhama K, Bhardwaj N and Sylvester A: Anti-neoplastic effect of chicken anemia virus VP3 protein (apoptin) in Rous sarcoma virus-induced tumours in chicken. J Gen Virol. 87:2933–2940. 2006. View Article : Google Scholar : PubMed/NCBI

16 

Du G, Ye L, Zhang G, Dong Q, Liu K and Tian J: Human IL18-IL2 fusion protein as a potential antitumor reagent by enhancing NK cell cytotoxicity and IFN-γ production. J Cancer Res Clin Oncol. 138:1727–1736. 2012. View Article : Google Scholar : PubMed/NCBI

17 

National Research Council (US) Institute for Laboratory Animal Research, . Guidance for the Description of Animal Research in Scientific Publications. National Academies Press; Washington DC: 2011

18 

Witlox MA, Lamfers ML, Wuisman PI, Curiel DT and Siegal GP: Evolving gene therapy approaches for osteosarcoma using viral vectors: Review. Bone. 40:797–812. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Pan D, Wei X, Liu M, Feng S, Tian X, Feng X and Zhang X: Adenovirus mediated transfer of p53, GM-CSF and B7-1 suppresses growth and enhances immunogenicity of glioma cells. Neurol Res. 32:502–509. 2010. View Article : Google Scholar : PubMed/NCBI

20 

Shimizu T, Shimada H, Ochiai T and Hamada H: Enhanced growth suppression in esophageal carcinoma cells using adenovirus-mediated fusion gene transfer (uracil phosphoribosyl transferase and herpes simplex virus thymidne kinase). Cancer Gene Ther. 8:512–521. 2001. View Article : Google Scholar : PubMed/NCBI

21 

Su H, Lu R, Ding R and Kan YW: Adeno-associated viral-mediated gene transfer to hepatoma: Thymidine kinase/interleukin 2 is more effective in tumor killing in non-ganciclovir (GCV)-treated than in GCV-treated animals. Mol Ther. 1:509–515. 2000. View Article : Google Scholar : PubMed/NCBI

22 

Cai BH, Chen JY, Lu MH, Chang LT, Lin HC, Chang YM and Chao CF: Functional four-base A/T gap core sequence CATTAG of P53 response elements specifically bound tetrameric P53 differently than two-base A/T gap core sequence CATG bound both dimeric and tetrameric P53. Nucleic Acids Res. 37:1984–1990. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Knoll S, Fürst K, Thomas S, Baselga S Villanueva, Stoll A, Schaefer S and Pützer BM: Dissection of cell context-dependent interactions between HBx and p53 family members in regulation of apoptosis: A role for HBV-induced HCC. Cell Cycle. 10:3554–3565. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Barneda-Zahonero B, Collazo O, Azagra A, Fernández-Duran I, Serra-Musach J, Islam AB, Vega-García N, Malatesta R, Camós M, Gómez A, et al: The transcriptional repressor HDAC7 promotes apoptosis and c-Myc downregulation in particular types of leukemia and lymphoma. Cell Death Dis. 6:e16352015. View Article : Google Scholar : PubMed/NCBI

25 

Cao Y, Yang G, Hunter ZR, Liu X, Xu L, Chen J, Tsakmaklis N, Hatjiharissi E, Kanan S, Davids MS, et al: The BCL2 antagonist ABT-199 triggers apoptosis, and augments ibrutinib and idelalisib mediated cytotoxicity in CXCR4 Wild-type and CXCR4 WHIM mutated Waldenstrom macroglobulinaemia cells. Br J Haematol. 170:134–138. 2015. View Article : Google Scholar : PubMed/NCBI

26 

Moon J, Koh SS, Malilas W, Cho IR, Kaewpiboon C, Kaowinn S, Lee K, Jhun BH, Choi YW and Chung YH: Acetylshikonin induces apoptosis of hepatitis B virus X protein-expressing human hepatocellular carcinoma cells via endoplasmic reticulum stress. Eur J Pharmacol. 735:132–140. 2014. View Article : Google Scholar : PubMed/NCBI

27 

Yang JJ, Zhang Y, Deng XZ, Xu K, Wang ZC, Wang J, Feng L and Ding WL: Influence of F protein of hepatitis C virus subtype 1b inhibits on human hepatocellular carcinoma HepG2 cell apoptosis. Zhonghua Liu Xing Bing Xue Za Zhi. 30:388–392. 2009.PubMed/NCBI

28 

Follstaedt SC, Barber SA and Zink MC: Mechanisms of minocycline-induced suppression of simian immunodeficiency virus encephalitis: Inhibition of apoptosis signal-regulating kinase 1. J Neurovirol. 14:376–388. 2008. View Article : Google Scholar : PubMed/NCBI

29 

Son KN and Lipton HL: Inhibition of Theiler's virus-induced apoptosis in infected murine macrophages results in necroptosis. Virus Res. 195:177–182. 2015. View Article : Google Scholar : PubMed/NCBI

30 

Kim ES, Khuri FR and Herbst RS: Epidermal growth factor receptor biology (IMC-C225). Curr Opin Oncol. 13:506–513. 2001. View Article : Google Scholar : PubMed/NCBI

31 

Lee SH, Kim MJ, Kim DW, Kang CD and Kim SH: Amurensin G enhances the susceptibility to tumor necrosis factor-related apoptosis-inducing ligand-mediated cytotoxicity of cancer stem-like cells of HCT-15 cells. Cancer Sci. 104:1632–1639. 2013. View Article : Google Scholar : PubMed/NCBI

32 

Youn YS, Shin MJ, Chae SY, Jin CH, Kim TH and Lee KC: Biological and physicochemical evaluation of the conformational stability of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). Biotechnol Lett. 29:713–721. 2007. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Chen LG, Liu YS, Zheng TH, Chen X, Li P, Xiao CX and Ren JL: Therapeutic targeting of liver cancer with a recombinant DNA vaccine containing the hemagglutinin-neuraminidase gene of Newcastle disease virus via apoptotic-dependent pathways. Oncol Lett 12: 3344-3350, 2016.
APA
Chen, L., Liu, Y., Zheng, T., Chen, X., Li, P., Xiao, C., & Ren, J. (2016). Therapeutic targeting of liver cancer with a recombinant DNA vaccine containing the hemagglutinin-neuraminidase gene of Newcastle disease virus via apoptotic-dependent pathways. Oncology Letters, 12, 3344-3350. https://doi.org/10.3892/ol.2016.5114
MLA
Chen, L., Liu, Y., Zheng, T., Chen, X., Li, P., Xiao, C., Ren, J."Therapeutic targeting of liver cancer with a recombinant DNA vaccine containing the hemagglutinin-neuraminidase gene of Newcastle disease virus via apoptotic-dependent pathways". Oncology Letters 12.5 (2016): 3344-3350.
Chicago
Chen, L., Liu, Y., Zheng, T., Chen, X., Li, P., Xiao, C., Ren, J."Therapeutic targeting of liver cancer with a recombinant DNA vaccine containing the hemagglutinin-neuraminidase gene of Newcastle disease virus via apoptotic-dependent pathways". Oncology Letters 12, no. 5 (2016): 3344-3350. https://doi.org/10.3892/ol.2016.5114
Copy and paste a formatted citation
x
Spandidos Publications style
Chen LG, Liu YS, Zheng TH, Chen X, Li P, Xiao CX and Ren JL: Therapeutic targeting of liver cancer with a recombinant DNA vaccine containing the hemagglutinin-neuraminidase gene of Newcastle disease virus via apoptotic-dependent pathways. Oncol Lett 12: 3344-3350, 2016.
APA
Chen, L., Liu, Y., Zheng, T., Chen, X., Li, P., Xiao, C., & Ren, J. (2016). Therapeutic targeting of liver cancer with a recombinant DNA vaccine containing the hemagglutinin-neuraminidase gene of Newcastle disease virus via apoptotic-dependent pathways. Oncology Letters, 12, 3344-3350. https://doi.org/10.3892/ol.2016.5114
MLA
Chen, L., Liu, Y., Zheng, T., Chen, X., Li, P., Xiao, C., Ren, J."Therapeutic targeting of liver cancer with a recombinant DNA vaccine containing the hemagglutinin-neuraminidase gene of Newcastle disease virus via apoptotic-dependent pathways". Oncology Letters 12.5 (2016): 3344-3350.
Chicago
Chen, L., Liu, Y., Zheng, T., Chen, X., Li, P., Xiao, C., Ren, J."Therapeutic targeting of liver cancer with a recombinant DNA vaccine containing the hemagglutinin-neuraminidase gene of Newcastle disease virus via apoptotic-dependent pathways". Oncology Letters 12, no. 5 (2016): 3344-3350. https://doi.org/10.3892/ol.2016.5114
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