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Article

Dysregulation of long non‑coding RNA profiles in human colorectal cancer and its association with overall survival

  • Authors:
    • Lei Yang
    • Lingling Xu
    • Qian Wang
    • Min Wang
    • Guangyu An
  • View Affiliations / Copyright

    Affiliations: Medical Research Center, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, P.R. China, Department of Oncology, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, P.R. China
  • Pages: 4068-4074
    |
    Published online on: September 15, 2016
       https://doi.org/10.3892/ol.2016.5138
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Abstract

Long non-coding RNAs (lncRNAs) emerged as key regulators of diverse roles during colorectal cancer (CRC) carcinogenesis, but their specific function still remains to be explored. The present study aimed to re‑annotate the Affymetrix Human Exon 1.0 ST Array for defining differential lncRNAs in CRC. Their prognostic relevance was also developed for screening key regulators in CRC. The CRC datasets E‑GEOD‑31737, E‑MATB‑829, Affymetrix colon cancer dataset and E‑GEOD‑24550 were re-purposed for searching differential lncRNAs and exploring their association with overall survival (OS). The identified lncRNAs were validated in CRC tissues or cell lines. As a result, 462, 286 and 166 differential lncRNAs were identified, respectively, in three predictive datasets. Among them, 48 lncRNAs were commonly observed to exhibit differential expression in the three datasets. Notably, the overexpression of family with sequence similarity 83 member H (FAM83H)‑antisense (AS) 1 (P=0.038) and VPS9 domain containing 1 (VPS9D1)‑AS1 (P=0.020) indicated shorter OS time than lower expression. The overexpression of FAM83H‑AS1 (P=0.033) and VPS9D1‑AS1 (P=0.011) was validated in cancerous tissues. Thus, FAM83H-AS1 and VPS9D1-AS1 may potentially enhance carcinogenesis or may be developed as prognostic biomarkers for CRC. In conclusion, a total of 48 CRC‑related lncRNAs were identified, the majority of which were confirmed to exhibit dysregulation. FAM83H‑AS1 and VPS9D1‑AS1 could have a potential use as prognostic biomarkers for CRC patients.
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Copy and paste a formatted citation
Spandidos Publications style
Yang L, Xu L, Wang Q, Wang M and An G: Dysregulation of long non‑coding RNA profiles in human colorectal cancer and its association with overall survival. Oncol Lett 12: 4068-4074, 2016.
APA
Yang, L., Xu, L., Wang, Q., Wang, M., & An, G. (2016). Dysregulation of long non‑coding RNA profiles in human colorectal cancer and its association with overall survival. Oncology Letters, 12, 4068-4074. https://doi.org/10.3892/ol.2016.5138
MLA
Yang, L., Xu, L., Wang, Q., Wang, M., An, G."Dysregulation of long non‑coding RNA profiles in human colorectal cancer and its association with overall survival". Oncology Letters 12.5 (2016): 4068-4074.
Chicago
Yang, L., Xu, L., Wang, Q., Wang, M., An, G."Dysregulation of long non‑coding RNA profiles in human colorectal cancer and its association with overall survival". Oncology Letters 12, no. 5 (2016): 4068-4074. https://doi.org/10.3892/ol.2016.5138
Copy and paste a formatted citation
x
Spandidos Publications style
Yang L, Xu L, Wang Q, Wang M and An G: Dysregulation of long non‑coding RNA profiles in human colorectal cancer and its association with overall survival. Oncol Lett 12: 4068-4074, 2016.
APA
Yang, L., Xu, L., Wang, Q., Wang, M., & An, G. (2016). Dysregulation of long non‑coding RNA profiles in human colorectal cancer and its association with overall survival. Oncology Letters, 12, 4068-4074. https://doi.org/10.3892/ol.2016.5138
MLA
Yang, L., Xu, L., Wang, Q., Wang, M., An, G."Dysregulation of long non‑coding RNA profiles in human colorectal cancer and its association with overall survival". Oncology Letters 12.5 (2016): 4068-4074.
Chicago
Yang, L., Xu, L., Wang, Q., Wang, M., An, G."Dysregulation of long non‑coding RNA profiles in human colorectal cancer and its association with overall survival". Oncology Letters 12, no. 5 (2016): 4068-4074. https://doi.org/10.3892/ol.2016.5138
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