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Article

Effects of microRNA-21 on the biological functions of T-cell acute lymphoblastic lymphoma/leukemia

  • Authors:
    • Cunzhen Shi
    • Xudong Zhang
    • Xin Li
    • Lei Zhang
    • Ling Li
    • Zhenchang Sun
    • Xiaorui Fu
    • Jingjing Wu
    • Yu Chang
    • Wencai Li
    • Qingjiang Chen
    • Mingzhi Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Institute of Clinical Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, Department of Pathology, Institute of Clinical Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China
  • Pages: 4173-4180
    |
    Published online on: September 21, 2016
       https://doi.org/10.3892/ol.2016.5163
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Abstract

Previous studies have demonstrated that microRNA-21 (miR-21) is an oncogene and is significantly upregulated in tumor tissue. However, its association with T‑cell acute lymphoblastic lymphoma/leukemia (T‑ALL) remains poorly understood. The aim of the present study was to investigate the effects of miR‑21 on T‑ALL cells by constructing Jurkat cells infected with recombinant adenovirus adv‑miR‑21 or adv‑anti‑miR‑21. In addition, the target gene of miR‑21 was identified by reverse transcription‑quantitative polymerase chain reaction and western blotting. The results demonstrated that miR‑21 expression in Jurkat cells was markedly upregulated. Furthermore, upregulating miR‑21 expression in Jurkat cells promoted cell proliferation and invasion and decreased the apoptosis rate. By contrast, knockdown of miR‑21 in Jurkat cells suppressed proliferation and invasion and increased the apoptosis rate. Furthermore, the results indicated that signal transducer and activator of transcription (STAT) 3 was targeted by miR‑21, and that miR‑21 inhibited STAT3 expression at the protein level rather than at the messenger RNA level. In conclusion, targeting the inhibition of miR‑21 may be a novel therapeutic strategy for patients with T-ALL.
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Copy and paste a formatted citation
Spandidos Publications style
Shi C, Zhang X, Li X, Zhang L, Li L, Sun Z, Fu X, Wu J, Chang Y, Li W, Li W, et al: Effects of microRNA-21 on the biological functions of T-cell acute lymphoblastic lymphoma/leukemia. Oncol Lett 12: 4173-4180, 2016.
APA
Shi, C., Zhang, X., Li, X., Zhang, L., Li, L., Sun, Z. ... Zhang, M. (2016). Effects of microRNA-21 on the biological functions of T-cell acute lymphoblastic lymphoma/leukemia. Oncology Letters, 12, 4173-4180. https://doi.org/10.3892/ol.2016.5163
MLA
Shi, C., Zhang, X., Li, X., Zhang, L., Li, L., Sun, Z., Fu, X., Wu, J., Chang, Y., Li, W., Chen, Q., Zhang, M."Effects of microRNA-21 on the biological functions of T-cell acute lymphoblastic lymphoma/leukemia". Oncology Letters 12.5 (2016): 4173-4180.
Chicago
Shi, C., Zhang, X., Li, X., Zhang, L., Li, L., Sun, Z., Fu, X., Wu, J., Chang, Y., Li, W., Chen, Q., Zhang, M."Effects of microRNA-21 on the biological functions of T-cell acute lymphoblastic lymphoma/leukemia". Oncology Letters 12, no. 5 (2016): 4173-4180. https://doi.org/10.3892/ol.2016.5163
Copy and paste a formatted citation
x
Spandidos Publications style
Shi C, Zhang X, Li X, Zhang L, Li L, Sun Z, Fu X, Wu J, Chang Y, Li W, Li W, et al: Effects of microRNA-21 on the biological functions of T-cell acute lymphoblastic lymphoma/leukemia. Oncol Lett 12: 4173-4180, 2016.
APA
Shi, C., Zhang, X., Li, X., Zhang, L., Li, L., Sun, Z. ... Zhang, M. (2016). Effects of microRNA-21 on the biological functions of T-cell acute lymphoblastic lymphoma/leukemia. Oncology Letters, 12, 4173-4180. https://doi.org/10.3892/ol.2016.5163
MLA
Shi, C., Zhang, X., Li, X., Zhang, L., Li, L., Sun, Z., Fu, X., Wu, J., Chang, Y., Li, W., Chen, Q., Zhang, M."Effects of microRNA-21 on the biological functions of T-cell acute lymphoblastic lymphoma/leukemia". Oncology Letters 12.5 (2016): 4173-4180.
Chicago
Shi, C., Zhang, X., Li, X., Zhang, L., Li, L., Sun, Z., Fu, X., Wu, J., Chang, Y., Li, W., Chen, Q., Zhang, M."Effects of microRNA-21 on the biological functions of T-cell acute lymphoblastic lymphoma/leukemia". Oncology Letters 12, no. 5 (2016): 4173-4180. https://doi.org/10.3892/ol.2016.5163
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