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Article

Anti-tumor and anti-invasion effects of a combination of 4-methylumbelliferone and ionizing radiation in human fibrosarcoma cells

  • Authors:
    • Ryo Saga
    • Satoru Monzen
    • Mitsuru Chiba
    • Hironori Yoshino
    • Toshiya Nakamura
    • Yoichiro Hosokawa
  • View Affiliations / Copyright

    Affiliations: Department of Radiological Life Sciences, Division of Medical Life Sciences, Hirosaki University Graduate School of Health Sciences, Hirosaki, Aomori 036‑8564, Japan, Department of Biomedical Sciences, Division of Medical Life Sciences, Hirosaki University Graduate School of Health Sciences, Hirosaki, Aomori 036‑8564, Japan
  • Pages: 410-416
    |
    Published online on: November 15, 2016
       https://doi.org/10.3892/ol.2016.5385
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Abstract

Hyaluronan (HA) is a major component of the extracellular matrix that is synthesized in excess in cancer tissues. 4‑methylumbelliferone (MU) inhibits the synthesis of HA and is closely related to the invasion and metastasis of cancer. However, the effects of MU in conjunction with cancer radiotherapy remain unknown. The present study assessed the anti‑tumor and anti‑invasion effects of the concomitant use of ionizing radiation (IR) and 100 µM MU on human fibrosarcoma HT1080 cells. Cell viability and cellular invasion potency assays were performed. There was a greater decrease in the viability of cells cultured with a combination of 2 Gy IR and MU compared with untreated control cells. In addition, cell cycle distribution analysis demonstrated that a higher proportion of these cells were in the sub‑G1 phase and higher fractions of annexin‑V positive, propidium iodide positive cells (i.e., apoptotic cells) were observed. HA concentration in the 2 Gy irradiated culture was similar to that in the non‑irradiated control culture, however, it significantly decreased following the administration of both MU alone and 2 Gy IR with MU. Furthermore, treatment with 2 Gy IR and MU resulted in a significant decrease in the invasion rate and matrix metalloproteinase (MMP)‑2 and MPP‑9 expression. Taken together, these results suggest that the administration of MU with 2 Gy IR is effective at reducing HA production, cell invasion and the metastatic potential of cancer cells.
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Copy and paste a formatted citation
Spandidos Publications style
Saga R, Monzen S, Chiba M, Yoshino H, Nakamura T and Hosokawa Y: Anti-tumor and anti-invasion effects of a combination of 4-methylumbelliferone and ionizing radiation in human fibrosarcoma cells. Oncol Lett 13: 410-416, 2017.
APA
Saga, R., Monzen, S., Chiba, M., Yoshino, H., Nakamura, T., & Hosokawa, Y. (2017). Anti-tumor and anti-invasion effects of a combination of 4-methylumbelliferone and ionizing radiation in human fibrosarcoma cells. Oncology Letters, 13, 410-416. https://doi.org/10.3892/ol.2016.5385
MLA
Saga, R., Monzen, S., Chiba, M., Yoshino, H., Nakamura, T., Hosokawa, Y."Anti-tumor and anti-invasion effects of a combination of 4-methylumbelliferone and ionizing radiation in human fibrosarcoma cells". Oncology Letters 13.1 (2017): 410-416.
Chicago
Saga, R., Monzen, S., Chiba, M., Yoshino, H., Nakamura, T., Hosokawa, Y."Anti-tumor and anti-invasion effects of a combination of 4-methylumbelliferone and ionizing radiation in human fibrosarcoma cells". Oncology Letters 13, no. 1 (2017): 410-416. https://doi.org/10.3892/ol.2016.5385
Copy and paste a formatted citation
x
Spandidos Publications style
Saga R, Monzen S, Chiba M, Yoshino H, Nakamura T and Hosokawa Y: Anti-tumor and anti-invasion effects of a combination of 4-methylumbelliferone and ionizing radiation in human fibrosarcoma cells. Oncol Lett 13: 410-416, 2017.
APA
Saga, R., Monzen, S., Chiba, M., Yoshino, H., Nakamura, T., & Hosokawa, Y. (2017). Anti-tumor and anti-invasion effects of a combination of 4-methylumbelliferone and ionizing radiation in human fibrosarcoma cells. Oncology Letters, 13, 410-416. https://doi.org/10.3892/ol.2016.5385
MLA
Saga, R., Monzen, S., Chiba, M., Yoshino, H., Nakamura, T., Hosokawa, Y."Anti-tumor and anti-invasion effects of a combination of 4-methylumbelliferone and ionizing radiation in human fibrosarcoma cells". Oncology Letters 13.1 (2017): 410-416.
Chicago
Saga, R., Monzen, S., Chiba, M., Yoshino, H., Nakamura, T., Hosokawa, Y."Anti-tumor and anti-invasion effects of a combination of 4-methylumbelliferone and ionizing radiation in human fibrosarcoma cells". Oncology Letters 13, no. 1 (2017): 410-416. https://doi.org/10.3892/ol.2016.5385
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