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Article

Isoimperatorin induces apoptosis of the SGC-7901 human gastric cancer cell line via the mitochondria-mediated pathway

  • Authors:
    • Kehui Tong
    • Chang Xin
    • Wenzhong Chen
  • View Affiliations / Copyright

    Affiliations: Department of Oncological Surgery, Yinzhou People's Hospital, School of Medicine, Ningbo University, Ningbo, Zhejiang 315040, P.R. China, Department of Hepatobiliary Surgery, Yinzhou People's Hospital, School of Medicine, Ningbo University, Ningbo, Zhejiang 315040, P.R. China, Department of General Surgery, Yinzhou People's Hospital, School of Medicine, Ningbo University, Ningbo, Zhejiang 315040, P.R. China
  • Pages: 518-524
    |
    Published online on: November 15, 2016
       https://doi.org/10.3892/ol.2016.5387
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Abstract

The present study was designed to investigate the antiproliferative activity of isoimperatorin against SGC‑7901 cells and to examine the possible mechanisms. The antiproliferative activity of isoimperatorin against SGC‑7901 cells was evaluated using an MTT assay, and the mechanisms were investigated using flow cytometry and western blot assays, which were used to determine the apoptotic rate and expression levels of mitochondria‑mediated apoptosis‑associated proteins, including Survivin, myeloid leukemia cell‑1 (Mcl‑1), B cell lymphoma‑extra large (Bcl‑xl), B cell lymphoma 2 (Bcl-2), second mitochondria‑derived activator of caspase (Smac), Bcl-2-associated X factor (Bax), cleaved (c)-caspase‑3 and c-caspase-9 in SGC‑7901 cells. Additionally, a xenograft assay was used to confirm whether isoimperatorin had an inhibitory effect on SGC‑7901 cell‑induced tumors in vivo. The results of the MTT assay suggested that isoimperatorin significantly inhibited the proliferation of SGC‑7901 cells in a dose‑ and time‑dependent manner, and the half maximal inhibitory concentration was 18.75 µg/ml. The results of the flow cytometric analysis indicated that, following treatment with isoimperatorin, the apoptotic rate of SGC‑7901 cells was significantly increased, compared with that of cells in the control group. The results of the western blot analysis indicated that, following treatment with isoimperatorin, the expression levels of the pro‑apoptotic proteins, Bax, c‑caspase‑3 and c‑caspase‑9, were significantly increased and the expression levels of the anti‑apoptotic proteins, Survivin and Bcl‑2, were significantly reduced, compared with the control group. No alterations in expression were found in the other apoptosis‑associated proteins, including Mcl‑1, Bcl‑xl and Smac. The results of the xenograft assay indicated that isoimperatorin significantly inhibited the growth of SGC‑7901 cell‑induced tumor in vivo by increasing the expression levels of pro‑apoptotic proteins (Bax, c-caspase‑3 and c-caspase‑9) and reducing the expression levels of anti‑apoptotic proteins (Survivin and Bcl‑2) without adverse effects on the increasing body weight of nude mice. In conclusion, the present study revealed that isoimperatorin may be able to induce the apoptosis of SGC‑7901 cells in vitro and in vivo by regulating the expression levels of mitochondria‑mediated apoptosis-associated proteins.
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1 

Ries LAG, Melbert D, Krapcho M, Stinchcomb DG, Howlader N, Horner MJ, Mariotto A, Miller BA, Feuer EJ, Altekruse SF, et al: SEER cancer statistics review, 1975–2005. Bethesda MD: National Cancer Institute; pp. 1975–2005. 2008, https://hero.epa.gov/hero/index.cfm/reference/details/reference_id/730406

2 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Horton R: GBD 2010: Understanding disease, injury, and risk. Lancet. 380:2053–2054. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Yancik R and Ries LA: Cancer in older persons: An international issue in an aging world. Semin Oncol. 31:128–136. 2004. View Article : Google Scholar : PubMed/NCBI

5 

Hu G, Tuomilehto J, Silventoinen K, Barengo NC, Peltonen M and Jousilahti P: The effects of physical activity and body mass index on cardiovascular, cancer and all-cause mortality among 47 212 middle-aged Finnish men and women. Int J Obesity (Lond). 29:894–902. 2005. View Article : Google Scholar

6 

Moreira DM, Aronson WJ, Terris MK, Kane CJ, Amling CL, Cooperberg MR, Boffetta P and Freedland SJ: Cigarette smoking is associated with an in increased risk of biochemical disease recurrence, metastasis, castration-resistant prostate cancer, and mortality after radical prostatectomy: Results from the SEARCH database. Cancer. 120:197–204. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Speer AG, Thursfield VJ, Torn-Broers Y and Jefford M: Pancreatic cancer: Surgical management and outcomes after 6 years of follow-up. Med J Aust. 196:511–515. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Jia L, Ma S, Hou X, Wang X, Qased AB, Sun X, Liang N, Li H, Yi H, Kong D, et al: The synergistic effects of traditional Chinese herbs and radiotherapy for cancer treatment. Oncol Lett. 5:1439–1447. 2013.PubMed/NCBI

9 

Romiti A, Cox MC, Sarcina I, Di Rocco R, D'Antonio C, Barucca V and Marchetti P: Metronomic chemotherapy for cancer treatment: A decade of clinical studies. Cancer Chemother Pharmacol. 72:13–33. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Li X, Yang G, Li X, Zhang Y, Yang J, Chang J, Sun X, Zhou X, Guo Y, Xu Y, et al: Traditional Chinese medicine in cancer care: A review of controlled clinical studies published in Chinese. PLoS One. 8:e603382013. View Article : Google Scholar : PubMed/NCBI

11 

Gopalakrishnan G, Lepetre S, Maksimenko A, Mura S, Desmaële D and Couvreur P: Lipid-conjugation of endogenous neuropeptides: Improved biotherapy against human pancreatic cancer. Adv Healthc Mater. 4:1015–1022. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Vanneman M and Dranoff G: Combing immunotherapy and target therapies in cancer treatment. Nat Rev Cancer. 12:237–251. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Duarte S, Carle G, Faneca H, de Lima MC and Pierrefite-Carle V: Suicide gene therapy in cancer: Where do we stand now? Cancer Lett. 324:160–170. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Vrbova B and Vrba J: Microwave thermotherapy in cancer treatment: Evaluation of homogeneity of SAR distribution. Prog Electromagn Res. 129:181–195. 2012. View Article : Google Scholar

15 

Brown SB, Brown EA and Walker I: The present and future role of photodynamic therapy in cancer treatment. Lancet Oncol. 5:497–508. 2004. View Article : Google Scholar : PubMed/NCBI

16 

Niu HX, Du T, Xu ZF, Zhang XK and Wang RG: Role of wild type p53 and double suicide genes in interventional therapy of liver cancer in rabbits. Acta Cir Bras. 27:522–528. 2012. View Article : Google Scholar : PubMed/NCBI

17 

Nobili S, Lippi D, Witort E, Donnini M, Bausi L, Mini E and Capaccioli S: Natural compounds for cancer treatment and prevention. Pharmacol Res. 59:365–378. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Wang MY, Jia MR, Ma YY and Li XB: Pharmacological effect of four linear furocoumarins in radix Angelicae dahuricae. Nat Prod Res Dev. 22:485–489. 2010.

19 

Kwon YS, Kobayashi A, Kajiyama S, Kawazu K, Kanzaki H and Kim CM: Antimicrobial constituents of Angelica dahurica roots. Phytochemistry. 44:887–889. 1997. View Article : Google Scholar : PubMed/NCBI

20 

Li ZK, Liu MQ and Yang HJ: The study of vasoactive of pungent herbs' chemical constituents in Umbelliferae. Pharmacol Clin Chin Mat Med. 25:38–40. 2009.

21 

Okuyama T, Takata M, Nishino H, Nishino A, Takayasu J and Lwashima A: Studies on the antitumor-promoting activity of naturally occurring substances. II. Inhibition of tumor-promoter-enhanced phospholipid metabolism by umbelliferous materials. Chem Pharm Bull (Tokyo). 38:1084–1086. 1990. View Article : Google Scholar : PubMed/NCBI

22 

Zhang XX: Studies on the antitumor constituents of Notopterygium incisum. [dissertation]. Hebei Med Univ; pp. 1–117. 2009

23 

Kim YK, Kim YS and Ryu SY: Antiproliferative effect of furanocoumarins from the root of Angelica dahurica on cultured human tumor cell lines. Phytother Res. 21:288–290. 2007. View Article : Google Scholar : PubMed/NCBI

24 

National Research Council (US) Committee for the Update of the Guide for the Care and Use of Laboratory Animals, . Guide for the Care and Use of Laboratory Animals. 8th. National Academies Press (US); Washington, DC: 2011, PubMed/NCBI

25 

Zhao YL, Zhao LJ, Luo YX, Li X, Zhang YF, Liu XD, Luo YG and Zhong LL: Synergistic effect of radiation and traditional Chinese medicine Rhizoma Typhonii ethanol extract depends on p53 expression in treatment of lewis mouse lung cancer cells. Afr J Tradit Complement Altern Med. 12:109–114. 2015. View Article : Google Scholar

26 

Acquaviva R, Di Giacomo C, Sorrenti V, Galvano F, Santangelo R, Cardile V, Gangia S, D'Orazio N, Abraham NG and Vanella L: Antiproliferation effect of oleuropein in prostate cell lines. Int J Oncol. 41:31–38. 2012.PubMed/NCBI

27 

Lee J, Gupta S, Huang JS, Jayathilaka LP and Lee BS: HPLC-MTT assay: Anticancer activity of aqueous garlic extract is from allicin. Anal Biochem. 436:187–189. 2013. View Article : Google Scholar : PubMed/NCBI

28 

Li W, Li DY, Wang HD, Zheng ZJ, Hu J and Li ZZ: Juglans regis hexane extract exerts antitumor effect, apoptosis induction and cell circle arrest in prostate cancer cells in vitro. Trop J Pharm Res. 14:399–405. 2015. View Article : Google Scholar

29 

Zi FM, He JS, Li Y, Wu C, Yang L, Yang Y, Wang LJ, He DH, Zhao Y, Wu WJ, et al: Metformin displays anti-myeloma activity and synergistic effect with dexamethasone in in vitro and in vivo xenograft models. Cancer Lett. 356:443–453. 2015. View Article : Google Scholar : PubMed/NCBI

30 

Shi Y: A structure view of mitochondria-medated apoptosis. Nat Struct Biol. 8:394–401. 2001. View Article : Google Scholar : PubMed/NCBI

31 

Wang X: The expanding role of mitochondria in apoptosis. Genes Dev. 15:2922–2933. 2001.PubMed/NCBI

32 

Yang XK, Xu MY, Xu GS, Zhang YL and Xu ZX: In vitro and in vivo antitumor activity of scutebarbatine A on human lung carcinoma A549 lines. Molecules. 19:8740–8751. 2014. View Article : Google Scholar : PubMed/NCBI

33 

Kim J, Parrish AB, Kurokawa M, Matsuura K, Freel CD, Andersen JL, Johnson CE and Kornbluth S: Rsk-mediated phosphorylation and 14-3-3ε binding of Apaf-1 suppresses cytochrome c-induced apoptosis. EMBO J. 31:1279–1292. 2012. View Article : Google Scholar : PubMed/NCBI

34 

Du C, Fang M, Li Y, Li L and Wang X: Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition. Cell. 102:33–42. 2000. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Tong K, Xin C and Chen W: Isoimperatorin induces apoptosis of the SGC-7901 human gastric cancer cell line via the mitochondria-mediated pathway. Oncol Lett 13: 518-524, 2017.
APA
Tong, K., Xin, C., & Chen, W. (2017). Isoimperatorin induces apoptosis of the SGC-7901 human gastric cancer cell line via the mitochondria-mediated pathway. Oncology Letters, 13, 518-524. https://doi.org/10.3892/ol.2016.5387
MLA
Tong, K., Xin, C., Chen, W."Isoimperatorin induces apoptosis of the SGC-7901 human gastric cancer cell line via the mitochondria-mediated pathway". Oncology Letters 13.1 (2017): 518-524.
Chicago
Tong, K., Xin, C., Chen, W."Isoimperatorin induces apoptosis of the SGC-7901 human gastric cancer cell line via the mitochondria-mediated pathway". Oncology Letters 13, no. 1 (2017): 518-524. https://doi.org/10.3892/ol.2016.5387
Copy and paste a formatted citation
x
Spandidos Publications style
Tong K, Xin C and Chen W: Isoimperatorin induces apoptosis of the SGC-7901 human gastric cancer cell line via the mitochondria-mediated pathway. Oncol Lett 13: 518-524, 2017.
APA
Tong, K., Xin, C., & Chen, W. (2017). Isoimperatorin induces apoptosis of the SGC-7901 human gastric cancer cell line via the mitochondria-mediated pathway. Oncology Letters, 13, 518-524. https://doi.org/10.3892/ol.2016.5387
MLA
Tong, K., Xin, C., Chen, W."Isoimperatorin induces apoptosis of the SGC-7901 human gastric cancer cell line via the mitochondria-mediated pathway". Oncology Letters 13.1 (2017): 518-524.
Chicago
Tong, K., Xin, C., Chen, W."Isoimperatorin induces apoptosis of the SGC-7901 human gastric cancer cell line via the mitochondria-mediated pathway". Oncology Letters 13, no. 1 (2017): 518-524. https://doi.org/10.3892/ol.2016.5387
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