Open Access

Profiling of microRNAs in AML cells following overexpression or silencing of the VEGF gene

  • Authors:
    • Li Li
    • Lixia Zhu
    • Yungui Wang
    • De Zhou
    • Jingjing Zhu
    • Wanzhuo Xie
    • Xiujin Ye
  • View Affiliations

  • Published online on: November 22, 2016     https://doi.org/10.3892/ol.2016.5412
  • Pages: 105-110
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Acute myeloid leukemia (AML) is a disease of the hematopoietic progenitor cells associated with heterogeneous clonal proliferation. Vascular endothelial growth factor (VEGF) and its receptors play important roles in the regulation of angiogenesis during physiological and pathological processes. It is thought that AML cells have an autocrine VEGF pathway that contributes to the development and progression of AML. In addition, growing evidence has suggested that numerous microRNAs are involved in AML. The present study aimed to investigate the relationship between VEGF dysregulation and microRNA profiles in AML cells and patients. VEGF‑overexpressing and VEGF‑knockdown leukemia cells were constructed and changes in the patterns of microRNA expression were analyzed using a microRNA array. Subsequently, mononuclear cells from the blood of patients with AML showing high or low expression levels of VEGF were obtained and were used to assess the patterns of microRNA expression by reverse transcription‑quantitative polymerase chain reaction. The results of the present study suggested that downregulation of VEGF markedly altered the profile of microRNAs in AML cells, while upregulation of VEGF did not. Examination of clinical samples from patients with AML showed that several microRNAs were closely associated with the expression level of VEGF, including miR‑20a, miR‑93, miR‑16‑5p, miR‑17‑5p, miR‑124‑5p and miR‑17‑3p. These results suggested that VEGF may be a pivotal protein that can both receive and initiate signals in leukemia cells.
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January-2017
Volume 13 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Li L, Zhu L, Wang Y, Zhou D, Zhu J, Xie W and Ye X: Profiling of microRNAs in AML cells following overexpression or silencing of the VEGF gene. Oncol Lett 13: 105-110, 2017
APA
Li, L., Zhu, L., Wang, Y., Zhou, D., Zhu, J., Xie, W., & Ye, X. (2017). Profiling of microRNAs in AML cells following overexpression or silencing of the VEGF gene. Oncology Letters, 13, 105-110. https://doi.org/10.3892/ol.2016.5412
MLA
Li, L., Zhu, L., Wang, Y., Zhou, D., Zhu, J., Xie, W., Ye, X."Profiling of microRNAs in AML cells following overexpression or silencing of the VEGF gene". Oncology Letters 13.1 (2017): 105-110.
Chicago
Li, L., Zhu, L., Wang, Y., Zhou, D., Zhu, J., Xie, W., Ye, X."Profiling of microRNAs in AML cells following overexpression or silencing of the VEGF gene". Oncology Letters 13, no. 1 (2017): 105-110. https://doi.org/10.3892/ol.2016.5412