Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
January-2017 Volume 13 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January-2017 Volume 13 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Increased expression of S100A6 promotes cell proliferation in gastric cancer cells

  • Authors:
    • Xiao‑Hong Wang
    • Hong Du
    • Lin Li
    • Duan‑Fang Shao
    • Xi‑Yao Zhong
    • Ying Hu
    • Yi‑Qiang Liu
    • Xiao‑Fang Xing
    • Xiao‑Jing Cheng
    • Ting Guo
    • Shen Li
    • Zi‑Yu Li
    • Zhao‑De Bu
    • Xian‑Zi Wen
    • Lian‑Hai Zhang
    • Jia‑Fu Ji
  • View Affiliations / Copyright

    Affiliations: Department of Tissue Bank, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital and Institute, Beijing 100142, P.R. China, Gastrointestinal Cancer Translational Research Laboratory, Peking University Cancer Hospital and Institute, Beijing 100142, P.R. China, Department of Pathology, Peking University Cancer Hospital and Institute, Beijing 100142, P.R. China, Department of Surgery, Peking University Cancer Hospital and Institute, Beijing 100142, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 222-230
    |
    Published online on: November 22, 2016
       https://doi.org/10.3892/ol.2016.5419
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

S100A6 is involved in regulating the progression of cancer. S100A6 can regulate the dynamics of cytoskeletal constituents, cell growth and differentiation by interacting with binding or target proteins. The present study investigated whether S100A6 affects cell proliferation in gastric cancer cells by stimulating several downstream factors. Firstly, the expression and localization of S100A6 were investigated using immunohistochemical staining, an immunoelectron microscopy and laser confocal scanning. A ChIP‑Chip assay was performed to determine the downstream factors of S100A6 using promoter Chip analysis, including approximately the ‑800 to +200 regions around the transcription starting point. Polymerase chain reaction analysis was performed to confirm this. It was found that the intensity of S100A6 staining was markedly higher in the cytoplasm and nucleus, and its expression level correlated with that of the Ki67 protein. The overexpression of S100A6 also promoted cell proliferation in AGS and BGC823 cell lines, detected using a Cell Counting‑Kit 8 assay. In cells overexpressing S100A6, the expression levels of interleukin (IL)‑8, cyclin‑dependent kinase (CDK)5, CDK4, minichromosome maintenance complex component 7 (MCM7) and B‑cell lymphoma 2 (Bcl2) were noticeably increased. In conclusion, the increased expression of S100A6 promoted cell proliferation by regulating the expression levels of IL‑8, CDK5, CDK4, MCM7 and Bcl2 in gastric cancer cells.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Cross SS, Hamdy FC, Deloulme JC and Rehman I: Expression of S100 proteins in normal human tissues and common cancers using tissue microarrays: S100A6, S100A8, S100A9 and S100A11 are all overexpressed in common cancers. Histopathology. 46:256–269. 2005. View Article : Google Scholar : PubMed/NCBI

2 

Wang XH, Zhang LH, Zhong XY, Xing XF, Liu YQ, Niu ZJ, Peng Y, Du H, Zhang GG, Hu Y, et al: S100A6 overexpression is associated with poor prognosis and is epigenetically up-regulated in gastric cancer. Am J Pathol. 177:586–597. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Filipek A and Kuźnicki J: Calcyclin-from basic research to clinical implications. Acta Biochim Pol. 40:321–327. 1993.PubMed/NCBI

4 

Shekouh AR, Thompson CC, Prime W, Campbell F, Hamlett J, Herrington CS, Lemoine NR, Crnogorac-Jurcevic T, Buechler MW, Friess H, et al: Application of laser capture microdissection combined with two-dimensional electrophoresis for the discovery of differentially regulated proteins in pancreatic ductal adenocarcinoma. Proteomics. 3:1988–2001. 2003. View Article : Google Scholar : PubMed/NCBI

5 

Brown LM, Helmke SM, Hunsucker SW, Netea-Maier RT, Chiang SA, Heinz DE, Shroyer KR, Duncan MW and Haugen BR: Quantitative and qualitative differences in protein expression between papillary thyroid carcinoma and normal thyroid tissue. Mol Carcinog. 45:613–626. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Stulik J, Osterreicher J, Koupilová K, Knízek J, Bures J, Jandík P, Langr F, Dedic K, Schäfer BW and Heizmann CW: Differential expression of the Ca2+ binding S100A6 protein in normal, preneoplastic and neoplastic colon mucosa. Eur J Cancer. 36:1050–1059. 2000. View Article : Google Scholar : PubMed/NCBI

7 

Komatsu K, Kobune-Fujiwara Y, Andoh A, Ishiguro S, Hunai H, Suzuki N, Kameyama M, Murata K, Miyoshi J, Akedo H, et al: Increased expression of S100A6 at the invading fronts of the primary lesion and liver metastasis in patients with colorectal adenocarcinoma. Br J Cancer. 83:769–774. 2000. View Article : Google Scholar : PubMed/NCBI

8 

Weterman MA, Stoopen GM, van Muijen GN, Kuznicki J, Ruiter DJ and Bloemers HP: Expression of calcyclin in human melanoma cell lines correlates with metastatic behavior in nude mice. Cancer Res. 52:1291–1296. 1992.PubMed/NCBI

9 

Pedrocchi M, Schafer BW, Mueller H, Eppenberger U and Heizmann CW: Expression of Ca (2+)-binding proteins of the S100 family in malignant human breast-cancer cell lines and biopsy samples. Int J Cancer. 57:684–690. 1994. View Article : Google Scholar : PubMed/NCBI

10 

Rehman I, Cross SS, Catto JW, Leiblich A, Mukherjee A, Azzouzi AR, Leung HY and Hamdy FC: Promoter hyper-methylation of calcium binding proteins S100A6 and S100A2 in human prostate cancer. Prostate. 65:322–330. 2005. View Article : Google Scholar : PubMed/NCBI

11 

Rehman I, Cross SS, Azzouzi AR, Catto JW, Deloulme JC, Larre S, Champigneuille J, Fromont G, Cussenot O and Hamdy FC: S100A6 (Calcyclin) is a prostate basal cell marker absent in prostate cancer and its precursors. Br J Cancer. 91:739–744. 2004.PubMed/NCBI

12 

Kim J, Kim J, Yoon S, Joo J, Lee Y, Lee K, Chung J and Choe I: S100A6 protein as a marker for differential diagnosis of cholangiocarcinoma from hepatocellular carcinoma. Hepatol Res. 23:2742002. View Article : Google Scholar : PubMed/NCBI

13 

Lyu X, Li H, Ma X, Li X, Gao Y, Ni D, Shen D, Gu L, Wang B, Zhang Y and Zhang X: High-level S100A6 promotes metastasis and predicts the outcome of T1-T2 stage in clear cell renal cell carcinoma. Cell Biochem Biophys. 71:279–290. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Emberley ED, Murphy LC and Watson PH: S100 proteins and their influence on pro-survival pathways in cancer. Biochem Cell Biol. 82:508–515. 2004. View Article : Google Scholar : PubMed/NCBI

15 

Golitsina NL, Kordowska J, Wang CL and Lehrer SS: Ca2+-dependent binding of calcyclin to muscle tropomyosin. Biochem Biophys Res Commun. 220:360–365. 1996. View Article : Google Scholar : PubMed/NCBI

16 

Mani RS, McCubbin WD and Kay CM: Calcium-dependent regulation of caldesmon by an 11-kDa smooth muscle calcium-binding protein, caltropin. Biochemistry. 31:11896–11901. 1992. View Article : Google Scholar : PubMed/NCBI

17 

Bao L, Odell AF, Stephen SL, Wheatcroft SB, Walker JH and Ponnambalam S: The S100A6 calcium-binding protein regulates endothelial cell-cycle progression and senescence. FEBS J. 279:4576–4588. 2012. View Article : Google Scholar : PubMed/NCBI

18 

Joo JH, Kim JW, Lee Y, Yoon SY, Kim JH, Paik SG and Choe IS: Involvement of NF-kappaB in the regulation of S100A6 gene expression in human hepatoblastoma cell line HepG2. Biochem Biophys Res Commun. 307:274–280. 2003. View Article : Google Scholar : PubMed/NCBI

19 

Zheng M, Barrera LO, Ren B and Wu YN: ChIP-chip: Data, model, and analysis. Biometrics. 63:787–796. 2007. View Article : Google Scholar : PubMed/NCBI

20 

Vimalachandran D, Greenhalf W, Thompson C, Lüttges J, Prime W, Campbell F, Dodson A, Watson R, Crnogorac-Jurcevic T, Lemoine N, et al: High nuclear S100A6 (Calcyclin) is significantly associated with poor survival in pancreatic cancer patients. Cancer Res. 65:3218–3225. 2005.PubMed/NCBI

21 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

22 

Wu H, Weng D, Weng D, Xing H, Song X, Zhu T, Xia X, Weng Y, Xu G, Meng L, et al: Reversal of the malignant phenotype of ovarian cancer A2780 cells through transfection with wild-type PTEN gene. Cancer Lett. 271:205–214. 2008. View Article : Google Scholar : PubMed/NCBI

23 

Leśniak W, Słomnicki ŁP and Filipek A: S100A6 - new facts and features. Biochem Biophys Res Commun. 390:1087–1092. 2009. View Article : Google Scholar : PubMed/NCBI

24 

Itoh Y, Joh T, Tanida S, Sasaki M, Kataoka H, Itoh K, Oshima T, Ogasawara N, Togawa S, Wada T, et al: IL-8 promotes cell proliferation and migration through metalloproteinase-cleavage proHB-EGF in human colon carcinoma cells. Cytokine. 29:275–282. 2005.PubMed/NCBI

25 

Lord JD, McIntosh BC, Greenberg PD and Nelson BH: The IL-2 receptor promotes lymphocyte proliferation and induction of the c-myc, bcl-2, and bcl-x genes through the trans-activation domain of Stat5. J Immunol. 164:2533–2541. 2000. View Article : Google Scholar : PubMed/NCBI

26 

Henriksson JT, Coursey TG, Corry DB, De Paiva CS and Pflugfelder SC: IL-13 stimulates proliferation and expression of mucin and immunomodulatory genes in cultured conjunctival goblet cells. Invest Ophthalmol Vis Sci. 56:4186–4197. 2015. View Article : Google Scholar : PubMed/NCBI

27 

Liu D, Liu C, Wang X, Ingvarsson S and Chen H: MicroRNA-451 suppresses tumor cell growth by down-regulating IL6R gene expression. Cancer Epidemiol. 38:85–92. 2014. View Article : Google Scholar : PubMed/NCBI

28 

Sherr CJ: The Pezcoller lecture: Cancer cell cycles revisited. Cancer Res. 60:3689–3695. 2000.PubMed/NCBI

29 

Brinkkoetter PT, Olivier P, Wu JS, Henderson S, Krofft RD, Pippin JW, Hockenbery D, Roberts JM and Shankland SJ: Cyclin I activates Cdk5 and regulates expression of Bcl-2 and Bcl-XL in postmitotic mouse cells. J Clin Invest. 119:3089–3101. 2009. View Article : Google Scholar : PubMed/NCBI

30 

Padmanabhan V, Callas P, Philips G, Trainer TD and Beatty BG: DNA replication regulation protein Mcm7 as a marker of proliferation in prostate cancer. J Clin Pathol. 57:1057–1062. 2004. View Article : Google Scholar : PubMed/NCBI

31 

Bouchard C, Staller P and Eilers M: Control of cell proliferation by Myc. Trends Cell Biol. 8:202–206. 1998. View Article : Google Scholar : PubMed/NCBI

32 

Słomnicki ŁP, Nawrot B and Leśniak W: S100A6 binds p53 and affects its activity. Int J Biochem Cell Biol. 41:784–790. 2009. View Article : Google Scholar : PubMed/NCBI

33 

Innocente SA and Lee JM: p53 is a NF-Y- and p21-independent, Sp1-dependent repressor of cyclin B1 transcription. FEBS Lett. 579:1001–1007. 2005. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wang XH, Du H, Li L, Shao DF, Zhong XY, Hu Y, Liu YQ, Xing XF, Cheng XJ, Guo T, Guo T, et al: Increased expression of S100A6 promotes cell proliferation in gastric cancer cells. Oncol Lett 13: 222-230, 2017.
APA
Wang, X., Du, H., Li, L., Shao, D., Zhong, X., Hu, Y. ... Ji, J. (2017). Increased expression of S100A6 promotes cell proliferation in gastric cancer cells. Oncology Letters, 13, 222-230. https://doi.org/10.3892/ol.2016.5419
MLA
Wang, X., Du, H., Li, L., Shao, D., Zhong, X., Hu, Y., Liu, Y., Xing, X., Cheng, X., Guo, T., Li, S., Li, Z., Bu, Z., Wen, X., Zhang, L., Ji, J."Increased expression of S100A6 promotes cell proliferation in gastric cancer cells". Oncology Letters 13.1 (2017): 222-230.
Chicago
Wang, X., Du, H., Li, L., Shao, D., Zhong, X., Hu, Y., Liu, Y., Xing, X., Cheng, X., Guo, T., Li, S., Li, Z., Bu, Z., Wen, X., Zhang, L., Ji, J."Increased expression of S100A6 promotes cell proliferation in gastric cancer cells". Oncology Letters 13, no. 1 (2017): 222-230. https://doi.org/10.3892/ol.2016.5419
Copy and paste a formatted citation
x
Spandidos Publications style
Wang XH, Du H, Li L, Shao DF, Zhong XY, Hu Y, Liu YQ, Xing XF, Cheng XJ, Guo T, Guo T, et al: Increased expression of S100A6 promotes cell proliferation in gastric cancer cells. Oncol Lett 13: 222-230, 2017.
APA
Wang, X., Du, H., Li, L., Shao, D., Zhong, X., Hu, Y. ... Ji, J. (2017). Increased expression of S100A6 promotes cell proliferation in gastric cancer cells. Oncology Letters, 13, 222-230. https://doi.org/10.3892/ol.2016.5419
MLA
Wang, X., Du, H., Li, L., Shao, D., Zhong, X., Hu, Y., Liu, Y., Xing, X., Cheng, X., Guo, T., Li, S., Li, Z., Bu, Z., Wen, X., Zhang, L., Ji, J."Increased expression of S100A6 promotes cell proliferation in gastric cancer cells". Oncology Letters 13.1 (2017): 222-230.
Chicago
Wang, X., Du, H., Li, L., Shao, D., Zhong, X., Hu, Y., Liu, Y., Xing, X., Cheng, X., Guo, T., Li, S., Li, Z., Bu, Z., Wen, X., Zhang, L., Ji, J."Increased expression of S100A6 promotes cell proliferation in gastric cancer cells". Oncology Letters 13, no. 1 (2017): 222-230. https://doi.org/10.3892/ol.2016.5419
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team