miR‑433 reduces cell viability and promotes cell apoptosis by regulating MACC1 in colorectal cancer

Li,Jiaxin; Mao,Xuping; Wang,Xing; Miao,Ganggang; Li,Jiaxin

doi:10.3892/ol.2016.5445

miR‑433 reduces cell viability and promotes cell apoptosis by regulating MACC1 in colorectal cancer

Authors:
- Jiaxin Li
- Xuping Mao
- Xing Wang
- Ganggang Miao
View Affiliations

Affiliations: Department of General Surgery, Danyang Hospital Affiliated to Nantong University, Zhenjiang, Jiangsu 212300, P.R. China, Department of Hepatic Surgery, Jiangsu Provincial People's Hospital (The First Affiliated Hospital of Nanjing Medical University), Nanjing, Jiangsu 210029, P.R. China
Published online on: November 30, 2016 https://doi.org/10.3892/ol.2016.5445
Pages: 81-88
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

MicroRNAs (miRNAs) are reported to have important roles in regulating the progression of numerous human cancers, although little is known regarding the role of miRNAs in colorectal cancer. The present study aimed to investigate the role of microRNA‑433 (miR‑433) in colorectal cancer. The expression levels of miR‑433 and its target gene metastasis associated in colon cancer‑1 (MACC1) in colorectal cancer tissues were evaluated using reverse transcription‑quantitative polymerase chain reaction and western blotting. Furthermore, flow cytometry and MTT assays were used to examine the apoptosis, cell cycle distribution and viability of human colorectal cancer cells, and luciferase reporter and western blot assays were performed to verify the regulatory mechanism of miR‑433 on MACC1. In addition, caspase‑3 and caspase‑9 expression were examined using western blotting. It was demonstrated that miR‑433 expression was downregulated in colorectal cancer tissues and cell lines. Artificial upregulation of miR‑433 in colorectal cancer cell lines using miR‑433 mimics revealed that upregulation of miR‑433 was able to reduce the viability and promote the apoptosis of colorectal cancer cells by downregulating MACC1. Taken together, these results suggested that miR‑433 may have an important role in the pathogenesis of colorectal cancer.

View Figures

View References

1	Meyerhardt JA and Mayer RJ: Systemic therapy for colorectal cancer. N Engl J Med. 352:476–487. 2005. View Article : Google Scholar : PubMed/NCBI
2	Wang YX, Chen YR, Liu SS, Ye YP, Jiao HL, Wang SY, Xiao ZY, Wei WT, Qiu JF, Liang L, Liao WT and Ding YQ: MiR-384 inhibits human colorectal cancer metastasis by targeting KRAS and CDC42. Oncotarget. doi: 10.18632/oncotarget.12704, 2016 (Epub ahead of print).
3	Wei W, Yang Y, Cai J, Cui K, Li RX, Wang H, Shang X and Wei D: mir-30a-5p suppresses tumor metastasis of human colorectal cancer by targeting ITGB3. Cell Physiol Biochem. 39:1165–1176. 2016. View Article : Google Scholar : PubMed/NCBI
4	Shao Y, Zhang SQ, Quan F, Zhang PF and Wu SL: MicroRNA-145 inhibits the proliferation, migration and invasion of the human TCA8113 oral cancer line. Oncol Lett. 6:1636–1640. 2013.PubMed/NCBI
5	Yang L, Wang YL, Liu S, Zhang PP, Chen Z, Liu M and Tang H: miR-181b promotes cell proliferation and reduces apoptosis by repressing the expression of adenylyl cyclase 9 (AC9) in cervical cancer cells. FEBS Lett. 588:124–130. 2014. View Article : Google Scholar : PubMed/NCBI
6	Billeter AT, Barnett RE, Druen D, Polk HC Jr and van Berkel VH: MicroRNA as a new factor in lung and esophageal cancer. Semin Thorac Cardiovasc Surg. 24:155–165. 2012. View Article : Google Scholar : PubMed/NCBI
7	Weiner-Gorzel K, Dempsey E, Milewska M, McGoldrick A, Toh V, Walsh A, Lindsay S, Gubbins L, Cannon A, Sharpe D, et al: Overexpression of the microRNA miR-433 promotes resistance to paclitaxel through the induction of cellular senescence in ovarian cancer cells. Cancer Med. 4:745–758. 2015. View Article : Google Scholar : PubMed/NCBI
8	Yang Z, Tsuchiya H, Zhang Y, Hartnett ME and Wang L: MicroRNA-433 inhibits liver cancer cell migration by repressing the protein expression and function of cAMP response element-binding protein. J Biol Chem. 288:28893–28899. 2013. View Article : Google Scholar : PubMed/NCBI
9	Lin X, Rice KL, Buzzai M, Hexner E, Costa FF, Kilpivaara O, Mullally A, Soares MB, Ebert BL, Levine R and Licht JD: miR-433 is aberrantly expressed in myeloproliferative neoplasms and suppresses hematopoietic cell growth and differentiation. Leukemia. 27:344–352. 2013. View Article : Google Scholar : PubMed/NCBI
10	Guo LH, Li H, Wang F, Yu J and He JS: The tumor suppressor roles of miR-433 and miR-127 in gastric cancer. Int J Mol Sci. 14:14171–14184. 2013. View Article : Google Scholar : PubMed/NCBI
11	Ge SH, Wu XJ, Wang XH, Xing XF, Zhang LH, Zhu YB, Du H, Dong B, Hu Y and Ji JF: Over-expression of metastasis-associated in colon cancer-1 (MACC1) associates with better prognosis of gastric cancer patients. Chin J Cancer Res. 23:153–159. 2011. View Article : Google Scholar : PubMed/NCBI
12	Xie C, Wu J, Yun J, Lai J, Yuan Y, Gao Z, Li M, Li J and Song L: MACC1 as a prognostic biomarker for early-stage and AFP-normal hepatocellular carcinoma. PLoS One. 8:e642352013. View Article : Google Scholar : PubMed/NCBI
13	Wang Z, Li Z, Wu C, Wang Y, Xia Y, Chen L, Zhu Q and Chen Y: MACC1 overexpression predicts a poor prognosis for non-small cell lung cancer. Med Oncol. 31:7902014. View Article : Google Scholar : PubMed/NCBI
14	Zhu M, Xu Y, Mao X, Gao Y, Shao L and Yan F: Overexpression of metastasis-associated in colon cancer-1 associated with poor prognosis in patients with esophageal cancer. Pathol Oncol Res. 19:749–753. 2013. View Article : Google Scholar : PubMed/NCBI
15	Yang T, Kong B, Kuang YQ, Cheng L, Gu JW, Zhang JH, Shu HF, Yu SX, He WQ, Xing XM and Huang HD: Overexpression of MACC1 protein and its clinical implications in patients with glioma. Tumour Biol. 35:815–819. 2014. View Article : Google Scholar : PubMed/NCBI
16	Huang Y, Zhang H, Cai J, Fang L, Wu J, Ye C, Zhu X and Li M: Overexpression of MACC1 and Its significance in human breast cancer progression. Cell Biosci. 3:162013. View Article : Google Scholar : PubMed/NCBI
17	Zhen T, Dai S, Li H, Yang Y, Kang L, Shi H, Zhang F, Yang D, Cai S, He Y, et al: MACC1 promotes carcinogenesis of colorectal cancer via β-catenin signaling pathway. Oncotarget. 5:3756–3769. 2014. View Article : Google Scholar : PubMed/NCBI
18	Quirke P, Williams GT, Ectors N, Ensari A, Piard F and Nagtegaal I: The future of the TNM staging system in colorectal cancer: Time for a debate? Lancet Oncol. 8:651–657. 2007. View Article : Google Scholar : PubMed/NCBI
19	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
20	Xia Y, Zhu Y, Ma T, Pan C, Wang J, He Z, Li Z, Qia X and Chen Y: miR-204 functions as a tumor suppressor by regulating SIX1 in NSCLC. FEBS Letters. 588:3703–3712. 2014. View Article : Google Scholar : PubMed/NCBI
21	Kokoszyńska K, Kryński J, Rychlewski L and Wyrwicz LS: Unexpected domain composition of MACC1 links MET signaling and apoptosis. Acta Biochim Pol. 56:317–323. 2009.PubMed/NCBI
22	Wang Z, Ma B, Ji X, Deng Y, Zhang T, Zhang X, Gao H, Sun H, Wu H, Chen X and Zhao R: MicroRNA-378-5p suppresses cell proliferation and induces apoptosis in colorectal cancer cells by targeting BRAF. Cancer Cell Int. 15:402015. View Article : Google Scholar : PubMed/NCBI
23	Zhu M, Xu Y, Ge M, Gui Z and Yan F: Regulation of UHRF1 by microRNA-9 modulates colorectal cancer cell proliferation and apoptosis. Cancer Sci. 106:833–839. 2015. View Article : Google Scholar : PubMed/NCBI
24	Boardman LA: Overexpression of MACC1 leads to downstream activation of HGF/MET and potentiates metastasis and recurrence of colorectal cancer. Genome Med. 1:362009. View Article : Google Scholar : PubMed/NCBI
25	Shirahata A, Shinmura K, Kitamura Y, Sakuraba K, Yokomizo K, Goto T, Mizukami H, Saito M, Ishibashi K, Kigawa G, et al: MACC1 as a marker for advanced colorectal carcinoma. Anticancer Res. 30:2689–2692. 2010.PubMed/NCBI