Comparative proteomic analysis of paclitaxel resistance‑related proteins in human breast cancer cell lines

  • Authors:
    • Hiroya Fujioka
    • Akiko Sakai
    • Satoru Tanaka
    • Kosei Kimura
    • Akiko Miyamoto
    • Mitsuhiko Iwamoto
    • Kazuhisa Uchiyama
  • View Affiliations

  • Published online on: November 30, 2016     https://doi.org/10.3892/ol.2016.5455
  • Pages: 289-295
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Abstract

Paclitaxel is widely used to treat various cancers; however, resistance to this drug is a major obstacle to breast cancer chemotherapy. To identify the proteins involved in paclitaxel resistance, the present study compared the proteomes of MCF‑7 human breast cancer cells and its paclitaxel‑resistant subclone MCF‑7/PTX. Using two‑dimensional gel electrophoresis and matrix‑assisted laser desorption/ionization time of flight mass spectrometry, 11 upregulated and 12 downregulated proteins were identified in MCF‑7/PTX cells compared with the parental cell line. These 23 proteins were functionally classified as stress‑induced chaperones, metabolic enzymes and cytoskeletal proteins. The anti‑apoptotic proteins, stress‑70 protein, 78‑kD glucose‑regulated protein, peptidyl‑prolyl cis‑trans isomerase A (PPIA) and heterogeneous nuclear ribonucleoprotein H3, were also upregulated in MCF‑7/PTX cells. Notably, knockdown of the stress‑response chaperone PPIA using small interfering RNA in MCF‑7/PTX cells restored their sensitivity to paclitaxel. These findings indicated that PPIA may have an important role in paclitaxel resistance in MCF‑7/PTX cells.
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January-2017
Volume 13 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Fujioka H, Sakai A, Tanaka S, Kimura K, Miyamoto A, Iwamoto M and Uchiyama K: Comparative proteomic analysis of paclitaxel resistance‑related proteins in human breast cancer cell lines. Oncol Lett 13: 289-295, 2017
APA
Fujioka, H., Sakai, A., Tanaka, S., Kimura, K., Miyamoto, A., Iwamoto, M., & Uchiyama, K. (2017). Comparative proteomic analysis of paclitaxel resistance‑related proteins in human breast cancer cell lines. Oncology Letters, 13, 289-295. https://doi.org/10.3892/ol.2016.5455
MLA
Fujioka, H., Sakai, A., Tanaka, S., Kimura, K., Miyamoto, A., Iwamoto, M., Uchiyama, K."Comparative proteomic analysis of paclitaxel resistance‑related proteins in human breast cancer cell lines". Oncology Letters 13.1 (2017): 289-295.
Chicago
Fujioka, H., Sakai, A., Tanaka, S., Kimura, K., Miyamoto, A., Iwamoto, M., Uchiyama, K."Comparative proteomic analysis of paclitaxel resistance‑related proteins in human breast cancer cell lines". Oncology Letters 13, no. 1 (2017): 289-295. https://doi.org/10.3892/ol.2016.5455