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Article Open Access

Plasma miR‑145, miR‑20a, miR‑21 and miR‑223 as novel biomarkers for screening early‑stage non‑small cell lung cancer

  • Authors:
    • Hui Zhang
    • Feng Mao
    • Tuyang Shen
    • Qingquan Luo
    • Zhengping Ding
    • Liqiang Qian
    • Jia Huang
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, Shanghai Chest Hospital, Shanghai Lung Tumor Clinical Medical Center, Shanghai Jiaotong University, Shanghai 200030, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 669-676
    |
    Published online on: December 6, 2016
       https://doi.org/10.3892/ol.2016.5462
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Abstract

Non‑small cell lung cancer (NSCLC) is the leading cause of cancer‑related mortality in the world. Late diagnosis is one of the most significant reasons for the high mortality rate of lung cancer. The identification of microRNAs (miRNAs) has opened a new field for molecular diagnosis of cancer. The purpose of the present study was to investigate whether plasma miRNAs may be used as biomarkers for early‑stage NSCLC. A total of 232 participants, including 149 NSCLC patients and 83 healthy controls, were recruited between July 2012 and May 2014. We measured the levels of 10 miRNAs (miR‑30d, miR‑383, miR‑20a, miR‑145, miR‑221, miR‑25, miR‑223, miR‑21, miR‑126 and miR‑210) in plasma samples of 40 individuals (20 patients and 20 matched healthy controls) at the point of identification of disease, and 129 NSCLC patients and 83 healthy controls at the validation stage using reverse transcription‑quantitative polymerase chain reaction. Receiver operating characteristics (ROC) curves were generated for each possible combination of the miRNAs. We observed that the expression of plasma miR‑145, miR‑20a, miR‑21 and miR‑223 was significantly increased in the early‑stage NSCLC samples compared with controls. miRNAs have significant diagnostic value for early‑stage NSCLC. Combined ROC analyses using these four miRNAs revealed an elevated area under the ROC curve (AUC) of 0.897, with a sensitivity and specificity of 81.8 and 90.1%, respectively. This AUC helped in distinguishing early‑stage NSCLC. Furthermore, the levels of the four plasma miRNAs were significantly decreased following surgery (P<0.05). Altered expression of miR‑145, miR‑20a, miR‑21 and miR‑223 in plasma are of tumor origin, and the four miRNAs may represent potential novel non‑invasive biomarkers for early‑stage NSCLC.
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View References

1 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2015. CA Cancer J Clin. 65:5–29. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Ettinger DS, Akerley W, Bepler G, Blum MG, Chang A, Cheney RT, Chirieac LR, D'Amico TA, Demmy TL, Ganti AK, et al: Non-small cell lung cancer. J Natl Compr Canc Netw. 8:740–801. 2010.PubMed/NCBI

3 

Crinò L, Weder W, van Meerbeeck J and Felip E: ESMO Guidelines Working Group: Early stage and locally advanced (non-metastatic) non-small-cell lung cancer: ESMO Clinical Practice Guidelines for diagnosis, treatment and follow-up. Ann Oncol. 21:(Suppl 5). v103–v115. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Swensen SJ, Jett JR, Hartman TE, Midthun DE, Mandrekar SJ, Hillman SL, Sykes AM, Aughenbaugh GL, Bungum AO and Allen KL: CT screening for lung cancer: five-year prospective experience. Radiology. 235:259–265. 2005. View Article : Google Scholar : PubMed/NCBI

6 

Boeri M, Verri C, Conte D, Roz L, Modena P, Facchinetti F, Calabrò E, Croce CM, Pastorino U and Sozzi G: MicroRNA signatures in tissues and plasma predict development and prognosis of computed tomography detected lung cancer. Proc Natl Acad Sci USA. 108:3713–3718. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Chen X, Hu Z, Wang W, Ba Y, Ma L, Zhang C, Wang C, Ren Z, Zhao Y, Wu S, et al: Identification of ten serum microRNAs from a genome-wide serum microRNA expression profile as novel non-invasive biomarkers for nonsmall cell lung cancer diagnosis. Int J Cancer. 130:1620–1628. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Lee RC, Feinbaum RL and Ambros V: The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell. 75:843–854. 1993. View Article : Google Scholar : PubMed/NCBI

9 

Mitchell PS, Parkin RK, Kroh EM, Fritz BR, Wyman SK, Pogosova-Agadjanyan EL, Peterson A, Noteboom J, O'Briant KC, Allen A, et al: Circulating microRNAs as stable blood-based markers for cancer detection. Proc Natl Acad Sci USA. 105:10513–10518. 2008. View Article : Google Scholar : PubMed/NCBI

10 

Keller A, Leidinger P, Gislefoss R, Haugen A, Langseth H, Staehler P, Lenhof HP and Meese E: Stable serum miRNA profiles as potential tool for non-invasive lung cancer diagnosis. RNA Biol. 8:506–516. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Shen J, Todd NW, Zhang H, Yu L, Lingxiao X, Mei Y, Guarnera M, Liao J, Chou A, Lu CL, et al: Plasma microRNAs as potential biomarkers for non-small-cell lung cancer. Lab Invest. 91:579–587. 2011. View Article : Google Scholar : PubMed/NCBI

12 

Chen X, Ba Y, Ma L, Cai X, Yin Y, Wang K, Guo J, Zhang Y, Chen J, Guo X, et al: Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases. Cell Res. 18:997–1006. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Boeri M, Verri C, Conte D, Roz L, Modena P, Facchinetti F, Calabrò E, Croce CM, Pastorino U and Sozzi G: MicroRNA signatures in tissues and plasma predict development and prognosis of computed tomography detected lung cancer. Proc Natl Acad Sci USA. 108:3713–3718. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Silva J, Garcia V, Zaballos Á, Provencio M, Lombardía L, Almonacid L, García JM, Domínguez G, Peña C, Diaz R, et al: Vesicle-related microRNAs in plasma of nonsmall cell lung cancer patients and correlation with survival. Eur Respir J. 37:617–623. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Tsim S, O'Dowd CA, Milroy R and Davidson S: Staging of non-small cell lung cancer (NSCLC): a review. Respir Med. 104:1767–1774. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Wong TS, Liu XB, Wong BY, Ng RW, Yuen AP and Wei WI: Mature miR-184 as potential oncogenic microRNA of squamous cell carcinoma of tongue. Clin Cancer Res. 14:2588–2592. 2008. View Article : Google Scholar : PubMed/NCBI

17 

Huang Z, Huang D, Ni S, Peng Z, Sheng W and Du X: Plasma microRNAs are promising novel biomarkers for early detection of colorectal cancer. Int J Cancer. 127:118–126. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Kroh EM, Parkin RK, Mitchell PS and Tewari M: Analysis of circulating microRNA biomarkers in plasma and serum using quantitative reverse transcription-PCR (qRT-PCR). Methods. 50:298–301. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Liu CJ, Kao SY, Tu HF, Tsai MM, Chang KW and Lin SC: Increase of microRNA miR-31 level in plasma could be a potential marker of oral cancer. Oral Dis. 16:360–364. 2010. View Article : Google Scholar : PubMed/NCBI

20 

Tsujiura M, Ichikawa D, Komatsu S, Shiozaki A, Takeshita H, Kosuga T, Konishi H, Morimura R, Deguchi K, Fujiwara H, et al: Circulating microRNAs in plasma of patients with gastric cancers. Br J Cancer. 102:1174–1179. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

22 

Russ R and Slack FJ: Cigarette-smoke-induced dysregulation of MicroRNA expression and its role in lung carcinogenesis. Pulm Med. 2012:7912342012. View Article : Google Scholar : PubMed/NCBI

23 

Wu X, Piper-Hunter MG, Crawford M, Nuovo GJ, Marsh CB, Otterson GA and Nana-Sinkam SP: MicroRNAs in the pathogenesis of lung cancer. J Thorac Oncol. 4:1028–1034. 2009. View Article : Google Scholar : PubMed/NCBI

24 

Swensen SJ, Jett JR, Sloan JA, Midthun DE, Hartman TE, Sykes AM, Aughenbaugh GL, Zink FE, Hillman SL, Noetzel GR, et al: Screening for lung cancer with low-dose spiral computed tomography. Am J Respir Crit Care Med. 165:508–513. 2002. View Article : Google Scholar : PubMed/NCBI

25 

Wieskopf B, Demangeat C, Purohit A, Stenger R, Gries P, Kreisman H and Quoix E: Cyfra 21-1 as a biologic marker of non-small cell lung cancer. Evaluation of sensitivity, specificity, and prognostic role. Chest. 108:163–169. 1995. View Article : Google Scholar : PubMed/NCBI

26 

Nisman B, Lafair J, Heching N, Lyass O, Baras M, Peretz T and Barak V: Evaluation of tissue polypeptide specific antigen, CYFRA 21-1, and carcinoembryonic antigen in nonsmall cell lung carcinoma: does the combined use of cytokeratin markers give any additional information? Cancer. 82:1850–1859. 1998. View Article : Google Scholar : PubMed/NCBI

27 

Andersen M, Grauslund M, Ravn J, Sørensen JB, Andersen CB and Santoni-Rugiu E: Diagnostic potential of miR-126, miR-143, miR-145, and miR-652 in malignant pleural mesothelioma. J Mol Diagn. 16:418–430. 2014. View Article : Google Scholar : PubMed/NCBI

28 

Zheng H, Zhang L, Zhao Y, Yang D, Song F, Wen Y, Hao Q, Hu Z, Zhang W and Chen K: Plasma miRNAs as diagnostic and prognostic biomarkers for ovarian cancer. PLoS One. 8:e778532013. View Article : Google Scholar : PubMed/NCBI

29 

Wang RJ, Zheng YH, Wang P and Zhang JZ: Serum miR-125a-5p, miR-145 and miR-146a as diagnostic biomarkers in non-small cell lung cancer. Int J Clin Exp Pathol. 8:765–771. 2015.PubMed/NCBI

30 

Foss KM, Sima C, Ugolini D, Neri M, Allen KE and Weiss GJ: miR-1254 and miR-574-5p: serum-based microRNA biomarkers for early-stage non-small cell lung cancer. J Thorac Oncol. 6:482–488. 2011. View Article : Google Scholar : PubMed/NCBI

31 

Volinia S, Calin GA, Liu CG, Ambs S, Cimmino A, Petrocca F, Visone R, Iorio M, Roldo C, Ferracin M, et al: A microRNA expression signature of human solid tumors defines cancer gene targets. Proc Natl Acad Sci USA. 103:2257–2261. 2006. View Article : Google Scholar : PubMed/NCBI

32 

Capodanno A, Boldrini L, Proietti A, Alì G, Pelliccioni S, Niccoli C, D'Incecco A, Cappuzzo F, Chella A, Lucchi M, et al: Let-7g and miR-21 expression in non-small cell lung cancer: correlation with clinicopathological and molecular features. Int J Oncol. 43:765–774. 2013.PubMed/NCBI

33 

Yang M, Shen H, Qiu C, Ni Y, Wang L, Dong W, Liao Y and Du J: High expression of miR-21 and miR-155 predicts recurrence and unfavourable survival in non-small cell lung cancer. Eur J Cancer. 49:604–615. 2013. View Article : Google Scholar : PubMed/NCBI

34 

O'Donnell KA, Wentzel EA, Zeller KI, Dang CV and Mendell JT: c-Myc-regulated microRNAs modulate E2F1 expression. Nature. 435:839–843. 2005. View Article : Google Scholar : PubMed/NCBI

35 

Chen Z, Zeng H, Guo Y, Liu P, Pan H, Deng A and Hu J: miRNA-145 inhibits non-small cell lung cancer cell proliferation by targeting c-Myc. J Exp Clin Cancer Res. 29:1512010. View Article : Google Scholar : PubMed/NCBI

36 

Zou C, Xu Q, Mao F, Li D, Bian C, Liu LZ, Jiang Y, Chen X, Qi Y, Zhang X, et al: MiR-145 inhibits tumor angiogenesis and growth by N-RAS and VEGF. Cell Cycle. 11:2137–2145. 2012. View Article : Google Scholar : PubMed/NCBI

37 

Mar-Aguilar F, Mendoza-Ramirez JA, Malagón-Santiago I, Espino-Silva PK, Santuario-Facio SK, Ruiz-Flores P, Rodríguez-Padilla C and Reséndez-Pérez D: Serum circulating microRNA profiling for identification of potential breast cancer biomarkers. Dis Markers. 34:163–169. 2013. View Article : Google Scholar : PubMed/NCBI

38 

Ng EK, Li R, Shin VY, Jin HC, Leung CP, Ma ES, Pang R, Chua D, Chu KM, Law WL, et al: Circulating microRNAs as specific biomarkers for breast cancer detection. PLoS One. 8:e531412013. View Article : Google Scholar : PubMed/NCBI

39 

Haneklaus M, Gerlic M, O'Neill LA and Masters SL: miR-223: infection, inflammation and cancer. J Intern Med. 274:215–226. 2013. View Article : Google Scholar : PubMed/NCBI

40 

Nian W, Ao X, Wu Y, Huang Y, Shao J, Wang Y, Chen Z, Chen F and Wang D: miR-223 functions as a potent tumor suppressor of the Lewis lung carcinoma cell line by targeting insulin-like growth factor-1 receptor and cyclin-dependent kinase 2. Oncol Lett. 6:359–366. 2013.PubMed/NCBI

41 

Lawrie CH, Gal S, Dunlop HM, Pushkaran B, Liggins AP, Pulford K, Banham AH, Pezzella F, Boultwood J, Wainscoat JS, et al: Detection of elevated levels of tumour-associated microRNAs in serum of patients with diffuse large B-cell lymphoma. Br J Haematol. 141:672–675. 2008. View Article : Google Scholar : PubMed/NCBI

42 

Heneghan HM, Miller N, Lowery AJ, Sweeney KJ, Newell J and Kerin MJ: Circulating microRNAs as novel minimally invasive biomarkers for breast cancer. Ann Surg. 251:499–505. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhang H, Mao F, Shen T, Luo Q, Ding Z, Qian L and Huang J: Plasma miR‑145, miR‑20a, miR‑21 and miR‑223 as novel biomarkers for screening early‑stage non‑small cell lung cancer. Oncol Lett 13: 669-676, 2017.
APA
Zhang, H., Mao, F., Shen, T., Luo, Q., Ding, Z., Qian, L., & Huang, J. (2017). Plasma miR‑145, miR‑20a, miR‑21 and miR‑223 as novel biomarkers for screening early‑stage non‑small cell lung cancer. Oncology Letters, 13, 669-676. https://doi.org/10.3892/ol.2016.5462
MLA
Zhang, H., Mao, F., Shen, T., Luo, Q., Ding, Z., Qian, L., Huang, J."Plasma miR‑145, miR‑20a, miR‑21 and miR‑223 as novel biomarkers for screening early‑stage non‑small cell lung cancer". Oncology Letters 13.2 (2017): 669-676.
Chicago
Zhang, H., Mao, F., Shen, T., Luo, Q., Ding, Z., Qian, L., Huang, J."Plasma miR‑145, miR‑20a, miR‑21 and miR‑223 as novel biomarkers for screening early‑stage non‑small cell lung cancer". Oncology Letters 13, no. 2 (2017): 669-676. https://doi.org/10.3892/ol.2016.5462
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang H, Mao F, Shen T, Luo Q, Ding Z, Qian L and Huang J: Plasma miR‑145, miR‑20a, miR‑21 and miR‑223 as novel biomarkers for screening early‑stage non‑small cell lung cancer. Oncol Lett 13: 669-676, 2017.
APA
Zhang, H., Mao, F., Shen, T., Luo, Q., Ding, Z., Qian, L., & Huang, J. (2017). Plasma miR‑145, miR‑20a, miR‑21 and miR‑223 as novel biomarkers for screening early‑stage non‑small cell lung cancer. Oncology Letters, 13, 669-676. https://doi.org/10.3892/ol.2016.5462
MLA
Zhang, H., Mao, F., Shen, T., Luo, Q., Ding, Z., Qian, L., Huang, J."Plasma miR‑145, miR‑20a, miR‑21 and miR‑223 as novel biomarkers for screening early‑stage non‑small cell lung cancer". Oncology Letters 13.2 (2017): 669-676.
Chicago
Zhang, H., Mao, F., Shen, T., Luo, Q., Ding, Z., Qian, L., Huang, J."Plasma miR‑145, miR‑20a, miR‑21 and miR‑223 as novel biomarkers for screening early‑stage non‑small cell lung cancer". Oncology Letters 13, no. 2 (2017): 669-676. https://doi.org/10.3892/ol.2016.5462
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