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Article Open Access

Effect of cancer/testis antigen NY‑SAR‑35 on the proliferation, migration and invasion of cancer cells

  • Authors:
    • Myung‑Ha Song
    • Ye‑Rin Kim
    • Jae‑Ho Bae
    • Chang‑Hun Lee
    • Sang‑Yull Lee
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry, School of Medicine, Pusan National University, Yangsan, Gyeongsangnam‑do 626‑870, Republic of Korea, Department of Pathology, School of Medicine, Pusan National University, Yangsan, Gyeongsangnam‑do 626‑870, Republic of Korea
    Copyright: © Song et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 784-790
    |
    Published online on: December 14, 2016
       https://doi.org/10.3892/ol.2016.5498
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Abstract

NY-SAR-35 is a cancer/testis (CT) antigen that was identified by serological analysis of recombinant complementary DNA expression libraries. The gene encoding NY‑SAR‑35 is located on the X chromosome and is aberrantly expressed in a number of cancer types and germ cells, such as those in the testes, but not in normal tissue. It has been reported that treatment with a demethylating agent induced the expression of NY‑SAR‑35 in several types of cancer cells. However, the function of NY‑SAR‑35 in cancer remains undetermined. In present study, the role of NY‑SAR‑35 in human lung adenocarcinoma (SK‑LC‑14) and hepatocellular carcinoma (SNU‑449) cells was investigated following stable transfection of the NY‑SAR‑35 gene. NY‑SAR‑35 was observed to be expressed in the cytoplasm of the cells. In addition, the bromodeoxyuridine incorporation assay and immunofluorescence staining for proliferating cell nuclear antigen and Ki‑67 demonstrated that proliferation was increased in cells transfected with NY‑SAR‑35. In addition, the trypan blue exclusion assay indicated that NY‑SAR‑35 increased cancer cell viability. Furthermore, NY‑SAR‑35 increased the migration and invasion of the cells. These results indicate that NY‑SAR‑35 increases cancer cell viability, proliferation, migration and invasion.
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Copy and paste a formatted citation
Spandidos Publications style
Song MH, Kim YR, Bae JH, Lee CH and Lee SY: Effect of cancer/testis antigen NY‑SAR‑35 on the proliferation, migration and invasion of cancer cells. Oncol Lett 13: 784-790, 2017.
APA
Song, M., Kim, Y., Bae, J., Lee, C., & Lee, S. (2017). Effect of cancer/testis antigen NY‑SAR‑35 on the proliferation, migration and invasion of cancer cells. Oncology Letters, 13, 784-790. https://doi.org/10.3892/ol.2016.5498
MLA
Song, M., Kim, Y., Bae, J., Lee, C., Lee, S."Effect of cancer/testis antigen NY‑SAR‑35 on the proliferation, migration and invasion of cancer cells". Oncology Letters 13.2 (2017): 784-790.
Chicago
Song, M., Kim, Y., Bae, J., Lee, C., Lee, S."Effect of cancer/testis antigen NY‑SAR‑35 on the proliferation, migration and invasion of cancer cells". Oncology Letters 13, no. 2 (2017): 784-790. https://doi.org/10.3892/ol.2016.5498
Copy and paste a formatted citation
x
Spandidos Publications style
Song MH, Kim YR, Bae JH, Lee CH and Lee SY: Effect of cancer/testis antigen NY‑SAR‑35 on the proliferation, migration and invasion of cancer cells. Oncol Lett 13: 784-790, 2017.
APA
Song, M., Kim, Y., Bae, J., Lee, C., & Lee, S. (2017). Effect of cancer/testis antigen NY‑SAR‑35 on the proliferation, migration and invasion of cancer cells. Oncology Letters, 13, 784-790. https://doi.org/10.3892/ol.2016.5498
MLA
Song, M., Kim, Y., Bae, J., Lee, C., Lee, S."Effect of cancer/testis antigen NY‑SAR‑35 on the proliferation, migration and invasion of cancer cells". Oncology Letters 13.2 (2017): 784-790.
Chicago
Song, M., Kim, Y., Bae, J., Lee, C., Lee, S."Effect of cancer/testis antigen NY‑SAR‑35 on the proliferation, migration and invasion of cancer cells". Oncology Letters 13, no. 2 (2017): 784-790. https://doi.org/10.3892/ol.2016.5498
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