Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
May-2017 Volume 13 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
May-2017 Volume 13 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Introduction of plasmids into gastric cancer cells by endoscopic ultrasound

  • Authors:
    • Minoru Tomizawa
    • Fuminobu Shinozaki
    • Yasufumi Motoyoshi
    • Takao Sugiyama
    • Shigenori Yamamoto
    • Naoki Ishige
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Chiba 284‑0003, Japan, Department of Radiology, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Chiba 284‑0003, Japan, Department of Neurology, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Chiba 284‑0003, Japan, Department of Rheumatology, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Chiba 284‑0003, Japan, Department of Pediatrics, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Chiba 284‑0003, Japan, Department of Neurosurgery, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Chiba 284‑0003, Japan
  • Pages: 3127-3130
    |
    Published online on: March 10, 2017
       https://doi.org/10.3892/ol.2017.5836
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Short hairpin RNA of frizzled-2 (shRNA-Fz2) suppresses the cell proliferation of gastric cancer cells. Endoscopic ultrasound (EUS) is considered a suitable method for the introduction of therapeutic plasmids into cells, since the device enables the access and real‑time monitoring of gastric cancer tissues. In the present study, plasmids were introduced into cells by sonoporation, as evidenced by the production of H2O2. The production of H2O2 was measured by absorbance of a potassium‑starch solution irradiated with EUS. Luciferase activity was analyzed in the cells irradiated with EUS after the addition of a pMetLuc2-control in the media, and cell proliferation was analyzed using a 3‑(4,5‑dimethylthiazol‑2‑yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H‑tetrazolium inner salt assay after irradiation with EUS following the addition of shRNA‑Fz2. Absorbance levels corresponding to free radical levels were found to be higher in the cells irradiated with EUS. Luciferase activities were found to be significantly higher in the transfected cells (plasmid with Lipofectamine LTX) than in untreated cells and were furthermore found to be higher in MKN45 cells irradiated for 0.5 min than in cells not subjected to irradiation. Luciferase activity was also found to be higher in MKN74 cells irradiated for 2 min than in cells that were not irradiated. Although the cell proliferation of the MKN45 cells tended to be suppressed by irradiation with EUS, this was non‑significant suppression, while the cell proliferation of MKN74 cells was found to be suppressed by irradiation with 12 MHz for 2 min (P<0.05). In conclusion, plasmids were introduced into cultured gastric cancer cells by irradiation with EUS due to sonoporation, as evidenced by the production of H2O2; however, the efficiency of the plasmid introduction was low compared with a traditional transfection approach.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Tanaka SS, Kojima Y, Yamaguchi YL, Nishinakamura R and Tam PP: Impact of WNT signaling on tissue lineage differentiation in the early mouse embryo. Dev Growth Differ. 53:843–856. 2011. View Article : Google Scholar : PubMed/NCBI

2 

MacDonald BT, Tamai K and He X: Wnt/beta-catenin signaling: Components, mechanisms and diseases. Dev Cell. 17:9–26. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Tomizawa M, Shinozaki F, Motoyoshi Y, Sugiyama T, Yamamoto S and Ishige N: Gastric cancer cell proliferation is suppressed by frizzled-2 short hairpin RNA. Int J Oncol. 46:1018–1024. 2015.PubMed/NCBI

4 

Feril LB Jr and Tachibana K: Use of ultrasound in drug delivery systems: Emphasis on experimental methodology and mechanisms. Int J Hyperthermia. 28:282–289. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Fechheimer M, Boylan JF, Parker S, Sisken JE, Patel GL and Zimmer SG: Transfection of mammalian cells with plasmid DNA by scrape loading and sonication loading. Proc Natl Acad Sci USA. 84:8463–8467. 1987. View Article : Google Scholar : PubMed/NCBI

6 

Kim HJ, Greenleaf JF, Kinnick RR, Bronk JT and Bolander ME: Ultrasound-mediated transfection of mammalian cells. Hum Gene Ther. 7:1339–1346. 1996. View Article : Google Scholar : PubMed/NCBI

7 

Tomizawa M, Ebara M, Saisho H, Sakiyama S and Tagawa M: Irradiation with ultrasound of low output intensity increased chemosensitivity of subcutaneous solid tumors to an anti-cancer agent. Cancer Lett. 173:31–35. 2001. View Article : Google Scholar : PubMed/NCBI

8 

O'Brien WD Jr: Ultrasound-biophysics mechanisms. Prog Biophys Mol Biol. 93:212–255. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Newman CM and Bettinger T: Gene therapy progress and prospects: Ultrasound for gene transfer. Gene Ther. 14:465–475. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Wang P, Li Y, Wang X, Guo L, Su X and Liu Q: Membrane damage effect of continuous wave ultrasound on K562 human leukemia cells. J Ultrasound Med. 31:1977–1986. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Okada K, Kudo N, Hassan MA, Kondo T and Yamamoto K: Threshold curves obtained under various gaseous conditions for free radical generation by burst ultrasound-Effects of dissolved gas, microbubbles and gas transport from the air. Ultrason Sonochem. 16:512–518. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Ebrahiminia A, Mokhtari-Dizaji M and Toliyat T: Correlation between iodide dosimetry and terephthalic acid dosimetry to evaluate the reactive radical production due to the acoustic cavitation activity. Ultrason Sonochem. 20:366–372. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Qiu Y, Zhang C, Tu J and Zhang D: Microbubble-induced sonoporation involved in ultrasound-mediated DNA transfection in vitro at low acoustic pressures. J Biomech. 45:1339–1345. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Kudo N, Okada K and Yamamoto K: Sonoporation by single-shot pulsed ultrasound with microbubbles adjacent to cells. Biophys J. 96:4866–4876. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Miller DL and Quddus J: Sonoporation of monolayer cells by diagnostic ultrasound activation of contrast-agent gas bodies. Ultrasound Med Biol. 26:661–667. 2000. View Article : Google Scholar : PubMed/NCBI

16 

Tomizawa M, Shinozaki F, Sugiyama T, Yamamoto S, Sueishi M and Yoshida T: Plasmid DNA introduced into cultured cells with diagnostic ultrasound. Oncol Rep. 27:1360–1364. 2012.PubMed/NCBI

17 

Tomizawa M, Shinozaki F, Sugiyama T, Yamamoto S, Sueishi M and Yoshida T: Short interference RNA introduced into cultured cells with diagnostic ultrasound. Oncol Rep. 27:65–68. 2012.PubMed/NCBI

18 

Tomizawa M, Shinozaki F, Hasegawa R, Fugo K, Shirai Y, Ichiki N, Sugiyama T, Yamamoto S, Sueishi M and Yoshida T: Screening ultrasonography is useful for the diagnosis of gastric and colorectal cancer. Hepatogastroenterology. 60:517–521. 2013.PubMed/NCBI

19 

El Abiad R and Gerke H: Gastric cancer: Endoscopic diagnosis and staging. Surg Oncol Clin N Am. 21:1–19. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Kondo T and Yoshii G: Effect of intensity of 1.2 MHz ultrasound on change in DNA synthesis of irradiated mouse L cells. Ultrasound Med Biol. 11:113–119. 1985. View Article : Google Scholar : PubMed/NCBI

21 

Tomizawa M, Shinozaki F, Motoyoshi Y, Sugiyama T, Yamamoto S and Sueishi M: Sonoporation: Gene transfer using ultrasound. World J Methodol. 3:39–44. 2013. View Article : Google Scholar : PubMed/NCBI

22 

WFUMB Symposium on safety and standardisation in medical ultrasound. Issues and recommendations regarding thermal mechanisms for biological effects of ultrasound. Hornbaek, denmark, 30 August-1. Ultrasound Med Biol. 18:731–810. 1992.PubMed/NCBI

23 

Hassan MA, Buldakov MA, Ogawa R, Zhao QL, Furusawa Y, Kudo N, Kondo T and Riesz P: Modulation control over ultrasound-mediated gene delivery: Evaluating the importance of standing waves. J Control Release. 141:70–76. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Kinoshita M and Hynynen K: Key factors that affect sonoporation efficiency in in vitro settings: The importance of standing wave in sonoporation. Biochem Biophys Res Commun. 359:860–865. 2007. View Article : Google Scholar : PubMed/NCBI

25 

Wang TS, Ding QQ, Guo RH, Shen H, Sun J, Lu KH, You SH, Ge HM, Shu YQ and Liu P: Expression of livin in gastric cancer and induction of apoptosis in SGC-7901 cells by shRNA-mediated silencing of livin gene. Biomed Pharmacother. 64:333–338. 2010. View Article : Google Scholar : PubMed/NCBI

26 

Yamaguchi K, Feril LB Jr, Tachibana K, Takahashi A, Matsuo M, Endo H, Harada Y and Nakayama J: Ultrasound-mediated interferon β gene transfection inhibits growth of malignant melanoma. Biochem Biophys Res Commun. 411:137–142. 2011. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Tomizawa M, Shinozaki F, Motoyoshi Y, Sugiyama T, Yamamoto S and Ishige N: Introduction of plasmids into gastric cancer cells by endoscopic ultrasound. Oncol Lett 13: 3127-3130, 2017.
APA
Tomizawa, M., Shinozaki, F., Motoyoshi, Y., Sugiyama, T., Yamamoto, S., & Ishige, N. (2017). Introduction of plasmids into gastric cancer cells by endoscopic ultrasound. Oncology Letters, 13, 3127-3130. https://doi.org/10.3892/ol.2017.5836
MLA
Tomizawa, M., Shinozaki, F., Motoyoshi, Y., Sugiyama, T., Yamamoto, S., Ishige, N."Introduction of plasmids into gastric cancer cells by endoscopic ultrasound". Oncology Letters 13.5 (2017): 3127-3130.
Chicago
Tomizawa, M., Shinozaki, F., Motoyoshi, Y., Sugiyama, T., Yamamoto, S., Ishige, N."Introduction of plasmids into gastric cancer cells by endoscopic ultrasound". Oncology Letters 13, no. 5 (2017): 3127-3130. https://doi.org/10.3892/ol.2017.5836
Copy and paste a formatted citation
x
Spandidos Publications style
Tomizawa M, Shinozaki F, Motoyoshi Y, Sugiyama T, Yamamoto S and Ishige N: Introduction of plasmids into gastric cancer cells by endoscopic ultrasound. Oncol Lett 13: 3127-3130, 2017.
APA
Tomizawa, M., Shinozaki, F., Motoyoshi, Y., Sugiyama, T., Yamamoto, S., & Ishige, N. (2017). Introduction of plasmids into gastric cancer cells by endoscopic ultrasound. Oncology Letters, 13, 3127-3130. https://doi.org/10.3892/ol.2017.5836
MLA
Tomizawa, M., Shinozaki, F., Motoyoshi, Y., Sugiyama, T., Yamamoto, S., Ishige, N."Introduction of plasmids into gastric cancer cells by endoscopic ultrasound". Oncology Letters 13.5 (2017): 3127-3130.
Chicago
Tomizawa, M., Shinozaki, F., Motoyoshi, Y., Sugiyama, T., Yamamoto, S., Ishige, N."Introduction of plasmids into gastric cancer cells by endoscopic ultrasound". Oncology Letters 13, no. 5 (2017): 3127-3130. https://doi.org/10.3892/ol.2017.5836
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team